Align phosphoribosyl-ATP diphosphatase (EC 3.6.1.31) (characterized)
to candidate AZOBR_RS14500 AZOBR_RS14500 nucleoside triphosphate pyrophosphohydrolase
Query= metacyc::MONOMER-21148 (267 letters) >FitnessBrowser__azobra:AZOBR_RS14500 Length = 269 Score = 179 bits (454), Expect = 5e-50 Identities = 102/264 (38%), Positives = 150/264 (56%), Gaps = 9/264 (3%) Query: 7 SLARLTDVIDRLLAPEG-CPWDKEQTPESLCDYLVEECFELVEAIRSGNADEVREEMGDV 65 ++ RL DV+ RL P G CPWD EQT ++ + +EE +E+ +AI G+ +REE+GD+ Sbjct: 4 NIDRLIDVMARLRDPNGGCPWDLEQTYATIAPHTIEEAYEVADAIEKGDMSALREELGDL 63 Query: 66 MFLLAFLGRLYADKGAFTLDDAMANNAAKMIRRHPHVFSDTTYADRDEFLRNWESIK--- 122 +F + + ++ ++ F D+ A KMIRRHPHVF D WE K Sbjct: 64 LFQVVYYSQMAREETLFDFDEVAGVIADKMIRRHPHVFGAEEVKGADMQTSRWEDHKAAE 123 Query: 123 RAEKADAEGEPQGVYDSLPASLPPLLKAYRIHSKAARVGFTWPEDEDVERQVEAEWLEL- 181 RA KA EG + + A LP L +A ++ ++AARVGF W + D+ ++E E EL Sbjct: 124 RAAKAAEEGRAPSALEGVIAGLPALTRALKLQNRAARVGFDWTDARDILDKIEEEVRELR 183 Query: 182 --LDVLAGDDKAAQENELGDLIFSLVELGRRKGIKANTALDMTNLKFLRRFRRMEALARE 239 +D + D+ A +ELGDL+F+LV L RR ++ +AL TN KF RRF R+EAL E Sbjct: 184 HEMDSGSAPDRVA--DELGDLLFALVNLARRLKVEPESALRGTNAKFERRFHRIEALLSE 241 Query: 240 RGLDFPALSLDDKDELWNEAKAAE 263 +G +LD+ + W +AK E Sbjct: 242 QGRTPKEATLDEMEAFWQQAKREE 265 Lambda K H 0.318 0.134 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 222 Number of extensions: 15 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 267 Length of database: 269 Length adjustment: 25 Effective length of query: 242 Effective length of database: 244 Effective search space: 59048 Effective search space used: 59048 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory