Aligments for a candidate for prs in Azospirillum brasilense Sp245

GapMind for Amino acid biosynthesis

Aligments for a candidate for prs in Azospirillum brasilense Sp245

Align Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 (characterized)
to candidate AZOBR_RS31185 AZOBR_RS31185 ribose-phosphate pyrophosphokinase

Query= SwissProt::P0A1V6
         (315 letters)



>FitnessBrowser__azobra:AZOBR_RS31185
          Length = 311

 Score =  323 bits (828), Expect = 3e-93
 Identities = 167/312 (53%), Positives = 228/312 (73%), Gaps = 4/312 (1%)

Query: 4   MKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQINENVRGGDIFIIQSTCAPT 63
           MK+ AGN+   LA+ I+  L   L  A++ RFSD EV V+I ENVRG D+F++QST  P 
Sbjct: 1   MKVLAGNSNRPLAEAISTCLGVPLTKASIRRFSDMEVFVEILENVRGEDVFVVQSTSFPA 60

Query: 64  NDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVRSARVPITAKVVADFLSSVGVD 123
           NDNLMEL+V +DALRR SA RITAV+PY+GYARQDR+    R PI+AK+VA+ ++  G +
Sbjct: 61  NDNLMELLVTIDALRRGSARRITAVLPYYGYARQDRKT-GPRTPISAKLVANLITQAGAN 119

Query: 124 RVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQL--NLDNPIVVSPDIGGVVRARAIA 181
           RVLT+DLHA QIQGFFD+P DN+  +P+  +D+     ++    +VSPD+GGVVRAR++A
Sbjct: 120 RVLTMDLHAGQIQGFFDIPTDNLMAAPVFEKDIRATFEDIGEVTMVSPDVGGVVRARSLA 179

Query: 182 KLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVLVDDMIDTGGTLCKAAEALKERGA 241
           K L D D+AIIDKRR RA VS+VM+IIGDV  R C+L+DD++D+GGTLC AA AL + GA
Sbjct: 180 KRL-DADLAIIDKRRERAGVSEVMNIIGDVKNRRCILLDDIVDSGGTLCNAAVALMDAGA 238

Query: 242 KRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIPLTDEIKALPNVRTLTLSGMLAEAIR 301
           K V AY TH + SG A   +  S ++++V  D+I  T+ ++   N+R LT++ +LA+AI 
Sbjct: 239 KSVHAYCTHGVLSGGAVARVAVSPLEQLVTTDSIQATEAVRVSRNIRQLTIAPLLADAIT 298

Query: 302 RISNEESISAMF 313
           RIS E S+S++F
Sbjct: 299 RISEERSVSSLF 310


Lambda     K      H
   0.322    0.137    0.386 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 290
Number of extensions: 14
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 315
Length of database: 311
Length adjustment: 27
Effective length of query: 288
Effective length of database: 284
Effective search space:    81792
Effective search space used:    81792
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 48 (23.1 bits)

Align candidate AZOBR_RS31185 AZOBR_RS31185 (ribose-phosphate pyrophosphokinase)
to HMM TIGR01251 (prs: ribose-phosphate diphosphokinase (EC 2.7.6.1))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01251.hmm
# target sequence database:        /tmp/gapView.8863.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01251  [M=309]
Accession:   TIGR01251
Description: ribP_PPkin: ribose-phosphate diphosphokinase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   1.2e-116  374.9   0.3   1.3e-116  374.8   0.3    1.0  1  lcl|FitnessBrowser__azobra:AZOBR_RS31185  AZOBR_RS31185 ribose-phosphate p


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__azobra:AZOBR_RS31185  AZOBR_RS31185 ribose-phosphate pyrophosphokinase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  374.8   0.3  1.3e-116  1.3e-116       1     309 []       1     311 []       1     311 [] 0.98

  Alignments for each domain:
  == domain 1  score: 374.8 bits;  conditional E-value: 1.3e-116
                                 TIGR01251   1 lkilsgssskelaekvaknlglelgdvevkkFadgElyvrieesvrgkdvfiivqstsapvndalmell 69 
                                               +k+l+g+s++ lae+++  lg++l+++++++F+d E+ v+i e+vrg+dvf++ qsts p nd+lmell
  lcl|FitnessBrowser__azobra:AZOBR_RS31185   1 MKVLAGNSNRPLAEAISTCLGVPLTKASIRRFSDMEVFVEILENVRGEDVFVV-QSTSFPANDNLMELL 68 
                                               799**************************************************.*************** PP

                                 TIGR01251  70 llidalkrasaksvtaviPyygYaRqdkkaksrepisaklvaklleeaGadrvltvdlHseqiqgfFdv 138
                                               ++idal+r sa+++tav+PyygYaRqd+k+ +r+pisaklva+l+++aGa+rvlt+dlH+ qiqgfFd+
  lcl|FitnessBrowser__azobra:AZOBR_RS31185  69 VTIDALRRGSARRITAVLPYYGYARQDRKTGPRTPISAKLVANLITQAGANRVLTMDLHAGQIQGFFDI 137
                                               ********************************************************************* PP

                                 TIGR01251 139 pvenlsaspklieelkkke..lknlvvvsPDkGaverakkvakklglelaiieKeRdskenevevtnll 205
                                               p +nl+a+p++ ++++++     ++++vsPD G+v ra+++ak+l+++laii+K+R+ ++ + ev+n++
  lcl|FitnessBrowser__azobra:AZOBR_RS31185 138 PTDNLMAAPVFEKDIRATFedIGEVTMVSPDVGGVVRARSLAKRLDADLAIIDKRRE-RAGVSEVMNII 205
                                               *****************99888899********************************.666******** PP

                                 TIGR01251 206 gdvegkdvvivDDiisTggTlvkaaelLkekGAkkvivaathgvfsgdAlerlaeagveevivtntilv 274
                                               gdv+++ ++++DDi+++ggTl++aa +L ++GAk+v +++thgv+sg A+ r+a + +e+++ t++i+ 
  lcl|FitnessBrowser__azobra:AZOBR_RS31185 206 GDVKNRRCILLDDIVDSGGTLCNAAVALMDAGAKSVHAYCTHGVLSGGAVARVAVSPLEQLVTTDSIQA 274
                                               ********************************************************************* PP

                                 TIGR01251 275 .ee.kklpkvseisvapliaeaiarihenesvsslfd 309
                                                e  +   +++++++apl+a+ai+ri+e++svsslf+
  lcl|FitnessBrowser__azobra:AZOBR_RS31185 275 tEAvRVSRNIRQLTIAPLLADAITRISEERSVSSLFS 311
                                               55588899***************************95 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (309 nodes)
Target sequences:                          1  (311 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 8.39
//
[ok]

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis