Alignments for a candidate for leuC in Azospirillum brasilense Sp245

GapMind for Amino acid biosynthesis

Alignments for a candidate for leuC in Azospirillum brasilense Sp245

Align 3-isopropylmalate dehydratase large subunit 1; Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1; EC 4.2.1.33 (characterized)
to candidate AZOBR_RS03545 AZOBR_RS03545 isopropylmalate isomerase

Query= SwissProt::P15717
         (466 letters)



>FitnessBrowser__azobra:AZOBR_RS03545
          Length = 467

 Score =  642 bits (1657), Expect = 0.0
 Identities = 318/463 (68%), Positives = 372/463 (80%)

Query: 3   KTLYEKLFDAHVVFEAPNETPLLYIDRHLVHEVTSPQAFDGLRAHHRPVRQPGKTFATMD 62
           +TL++K++D+HVV    + T +LYIDRHLVHEVTSPQAF+GLR   RPVR+P  T A  D
Sbjct: 5   RTLFDKIWDSHVVHRQDDGTCILYIDRHLVHEVTSPQAFEGLRLAGRPVRRPEATLAVPD 64

Query: 63  HNVSTQTKDINASGEMARIQMQELIKNCNEFGVELYDLNHPYQGIVHVMGPEQGVTLPGM 122
           HNV T  +      E +RIQ++ L KN  +FGV  + ++   QGIVH++GPEQG TLPG 
Sbjct: 65  HNVPTTDRSKGIVEEESRIQVETLDKNARDFGVRYFPMDDVRQGIVHIVGPEQGFTLPGA 124

Query: 123 TIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQGRAKTMKIEVTGNAAPGITAKD 182
           TIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTL Q  AK M + V G    G+TAKD
Sbjct: 125 TIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLLQKPAKNMLVRVDGELPAGVTAKD 184

Query: 183 IVLAIIGKTGSAGGTGHVVEFCGDAIRALSMEGRMTLCNMAIEMGAKAGLVAPDETTFNY 242
           IVLAIIG+ G+AGGTGHV+EF GDAIR LSMEGRMT+CNMAIE GA+AGL+APDE TF Y
Sbjct: 185 IVLAIIGRIGTAGGTGHVIEFAGDAIRGLSMEGRMTVCNMAIEGGARAGLIAPDEKTFEY 244

Query: 243 VKGRLHAPKGRDFDEAVEYWKTLKTDDGATFDTVVALRAEEIAPQVTWGTNPGQVISVTD 302
           VKGR  APK   +++AVEYWKTL +D+GA +DT V L A +I PQVTWGT+P  V+ +T 
Sbjct: 245 VKGRALAPKAGAWEQAVEYWKTLPSDEGAVYDTTVVLNAADIVPQVTWGTSPEDVLPITA 304

Query: 303 IIPDPASFSDPVERASAEKALAYMGLQPGVPLTDVAIDKVFIGSCTNSRIEDLRAAAEVA 362
            +P+PA  +D  +RA+ E++LAYMGL+PG  LTDV +D VFIGSCTN RIEDLRAAA +A
Sbjct: 305 TVPNPADVADAGKRAAVERSLAYMGLEPGQKLTDVKVDTVFIGSCTNGRIEDLRAAAVIA 364

Query: 363 KGRKVAPGVQALVVPGSGPVKAQAEAEGLDKIFIEAGFEWRLPGCSMCLAMNNDRLNPGE 422
           +GRKVA GV+ALVVPGSG VK QAE EGLDKIF EAGFEWR PGCSMCLAMN DRL+PGE
Sbjct: 365 QGRKVAEGVRALVVPGSGLVKDQAEEEGLDKIFTEAGFEWREPGCSMCLAMNADRLSPGE 424

Query: 423 RCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVTGHFADIRSI 465
           R ASTSNRNFEGRQGRGGRTHL+SPAMAAAAAVTGH  D+RS+
Sbjct: 425 RSASTSNRNFEGRQGRGGRTHLLSPAMAAAAAVTGHLTDVRSL 467


Lambda     K      H
   0.318    0.134    0.396 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 817
Number of extensions: 30
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 466
Length of database: 467
Length adjustment: 33
Effective length of query: 433
Effective length of database: 434
Effective search space:   187922
Effective search space used:   187922
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)

Align candidate AZOBR_RS03545 AZOBR_RS03545 (isopropylmalate isomerase)
to HMM TIGR00170 (leuC: 3-isopropylmalate dehydratase, large subunit (EC 4.2.1.33))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00170.hmm
# target sequence database:        /tmp/gapView.2545.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00170  [M=466]
Accession:   TIGR00170
Description: leuC: 3-isopropylmalate dehydratase, large subunit
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   2.7e-251  820.0   0.1   3.1e-251  819.8   0.1    1.0  1  lcl|FitnessBrowser__azobra:AZOBR_RS03545  AZOBR_RS03545 isopropylmalate is


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__azobra:AZOBR_RS03545  AZOBR_RS03545 isopropylmalate isomerase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  819.8   0.1  3.1e-251  3.1e-251       2     465 ..       4     466 ..       3     467 .] 1.00

  Alignments for each domain:
  == domain 1  score: 819.8 bits;  conditional E-value: 3.1e-251
                                 TIGR00170   2 aktlyeklfdahvvkeaenetdllyidrhlvhevtspqafeglraagrkvrrvdktlatldhnistesr 70 
                                               ++tl++k++d+hvv++++++t +lyidrhlvhevtspqafeglr agr+vrr++ tla  dhn++t+ r
  lcl|FitnessBrowser__azobra:AZOBR_RS03545   4 PRTLFDKIWDSHVVHRQDDGTCILYIDRHLVHEVTSPQAFEGLRLAGRPVRRPEATLAVPDHNVPTTDR 72 
                                               89******************************************************************* PP

                                 TIGR00170  71 dveikeekaklqvkeleknvkefgvklfdlssaeqgivhvvgpeegltlpgktivcgdshtathgafga 139
                                                  i+ee++++qv++l+kn+++fgv++f +++++qgivh+vgpe+g+tlpg tivcgdshtathgafga
  lcl|FitnessBrowser__azobra:AZOBR_RS03545  73 SKGIVEEESRIQVETLDKNARDFGVRYFPMDDVRQGIVHIVGPEQGFTLPGATIVCGDSHTATHGAFGA 141
                                               ********************************************************************* PP

                                 TIGR00170 140 lafgigtsevehvlatqtlkqaraktlkievegklakgitakdiilaiigkigvaggtgyvvefageai 208
                                               lafgigtsevehvlatqtl q+ ak++ ++v+g+l  g+takdi+laiig+ig+aggtg+v+efag+ai
  lcl|FitnessBrowser__azobra:AZOBR_RS03545 142 LAFGIGTSEVEHVLATQTLLQKPAKNMLVRVDGELPAGVTAKDIVLAIIGRIGTAGGTGHVIEFAGDAI 210
                                               ********************************************************************* PP

                                 TIGR00170 209 rdlsmeermtvcnmaieagakagliapdettfeyvkdrkyapkgkefekavaywktlktdegakfdkvv 277
                                               r lsme+rmtvcnmaie ga+agliapde tfeyvk+r +apk   +e+av+ywktl +dega++d++v
  lcl|FitnessBrowser__azobra:AZOBR_RS03545 211 RGLSMEGRMTVCNMAIEGGARAGLIAPDEKTFEYVKGRALAPKAGAWEQAVEYWKTLPSDEGAVYDTTV 279
                                               ********************************************************************* PP

                                 TIGR00170 278 tleakdispqvtwgtnpgqvlsvneevpdpksladpvekasaekalaylglepgtklkdikvdkvfigs 346
                                               +l+a di+pqvtwgt+p++vl+++ +vp+p+++ad  ++a +e++lay+glepg+kl+d+kvd vfigs
  lcl|FitnessBrowser__azobra:AZOBR_RS03545 280 VLNAADIVPQVTWGTSPEDVLPITATVPNPADVADAGKRAAVERSLAYMGLEPGQKLTDVKVDTVFIGS 348
                                               ********************************************************************* PP

                                 TIGR00170 347 ctnsriedlraaaevvkgkkvadnvklalvvpgsglvkkqaekegldkifleagfewreagcslclgmn 415
                                               ctn+riedlraaa +++g+kva++v+ alvvpgsglvk qae+egldkif eagfewre+gcs+cl+mn
  lcl|FitnessBrowser__azobra:AZOBR_RS03545 349 CTNGRIEDLRAAAVIAQGRKVAEGVR-ALVVPGSGLVKDQAEEEGLDKIFTEAGFEWREPGCSMCLAMN 416
                                               **************************.****************************************** PP

                                 TIGR00170 416 ndvldeyercastsnrnfegrqgkgarthlvspamaaaaavagkfvdire 465
                                                d+l+++er astsnrnfegrqg+g+rthl+spamaaaaav+g++ d+r 
  lcl|FitnessBrowser__azobra:AZOBR_RS03545 417 ADRLSPGERSASTSNRNFEGRQGRGGRTHLLSPAMAAAAAVTGHLTDVRS 466
                                               ************************************************97 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (466 nodes)
Target sequences:                          1  (467 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.02s 00:00:00.03 Elapsed: 00:00:00.02
# Mc/sec: 8.37
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis