Alignments for a candidate for lysT in Azospirillum brasilense Sp245

GapMind for Amino acid biosynthesis

Alignments for a candidate for lysT in Azospirillum brasilense Sp245

Align Probable methanogen homoaconitase large subunit; HACN; EC 4.2.1.114; Homoaconitate hydratase (uncharacterized)
to candidate AZOBR_RS03545 AZOBR_RS03545 isopropylmalate isomerase

Query= curated2:O27668
         (428 letters)



>FitnessBrowser__azobra:AZOBR_RS03545
          Length = 467

 Score =  226 bits (575), Expect = 1e-63
 Identities = 149/437 (34%), Positives = 214/437 (48%), Gaps = 57/437 (13%)

Query: 30  VDLAMTHDGTSPPTIRTFRDIASRGGPARVWDPERIVMVFDHNVPANTIGAAEFQRVTR- 88
           +D  + H+ TSP   + F  +   G P R   PE  + V DHNVP         +  +R 
Sbjct: 29  IDRHLVHEVTSP---QAFEGLRLAGRPVR--RPEATLAVPDHNVPTTDRSKGIVEEESRI 83

Query: 89  ------EFAREQGIVNIFQNAA--GICHQVLPERGFVRPGMVIVGADSHTCTYGAFGAFA 140
                 + AR+ G+     +    GI H V PE+GF  PG  IV  DSHT T+GAFGA A
Sbjct: 84  QVETLDKNARDFGVRYFPMDDVRQGIVHIVGPEQGFTLPGATIVCGDSHTATHGAFGALA 143

Query: 141 TGMGATDMAMVFATGKTWFMVPEAMRIEVTGEPEGHVYAKDVILHIIGEIGVDGATYRSV 200
            G+G +++  V AT        + M + V GE    V AKD++L IIG IG  G T   +
Sbjct: 144 FGIGTSEVEHVLATQTLLQKPAKNMLVRVDGELPAGVTAKDIVLAIIGRIGTAGGTGHVI 203

Query: 201 EFTGDTIESMDVSGRMTICNMAVEMGAKNGIMEPNRQTLDYVRAR-----------TGRE 249
           EF GD I  + + GRMT+CNMA+E GA+ G++ P+ +T +YV+ R               
Sbjct: 204 EFAGDAIRGLSMEGRMTVCNMAIEGGARAGLIAPDEKTFEYVKGRALAPKAGAWEQAVEY 263

Query: 250 FRVYSSDEDSQYLEDHHFDVSDLEPQVACPDDVDNVYPV--------------------- 288
           ++   SDE + Y      + +D+ PQV      ++V P+                     
Sbjct: 264 WKTLPSDEGAVYDTTVVLNAADIVPQVTWGTSPEDVLPITATVPNPADVADAGKRAAVER 323

Query: 289 ----------HRVEGTHIDEAFLGSCTNGRYEDLKIAAEVIGDRRVHEDVRFIVSPASRE 338
                      ++    +D  F+GSCTNGR EDL+ AA +   R+V E VR +V P S  
Sbjct: 324 SLAYMGLEPGQKLTDVKVDTVFIGSCTNGRIEDLRAAAVIAQGRKVAEGVRALVVPGSGL 383

Query: 339 IYLKALEDGIIETFIRAGAIVCNPGCGPCLGAHMGVLAPGEVSIATTNRNFRGRMGDPAS 398
           +  +A E+G+ + F  AG     PGC  CL  +   L+PGE S +T+NRNF GR G    
Sbjct: 384 VKDQAEEEGLDKIFTEAGFEWREPGCSMCLAMNADRLSPGERSASTSNRNFEGRQG-RGG 442

Query: 399 SVYLANPAVVAESAIEG 415
             +L +PA+ A +A+ G
Sbjct: 443 RTHLLSPAMAAAAAVTG 459


Lambda     K      H
   0.320    0.137    0.408 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 571
Number of extensions: 27
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 428
Length of database: 467
Length adjustment: 33
Effective length of query: 395
Effective length of database: 434
Effective search space:   171430
Effective search space used:   171430
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis