Align amino-acid N-acetyltransferase (EC 2.3.1.1) (characterized)
to candidate GFF3930 Psest_4000 amino-acid N-acetyltransferase
Query= BRENDA::P22567 (432 letters) >FitnessBrowser__psRCH2:GFF3930 Length = 432 Score = 751 bits (1940), Expect = 0.0 Identities = 375/432 (86%), Positives = 405/432 (93%) Query: 1 MPDYVNWLRHASPYINSHRDRTFVVMLPGEGVEHPNFGNIVHDLVLLHSLGARLVLVHGS 60 M DYV WLR +SPYINSHRDRTFVVMLPG+G+EH NF NIVHDLVLLHSLG RLVLV GS Sbjct: 1 MHDYVTWLRDSSPYINSHRDRTFVVMLPGDGLEHANFANIVHDLVLLHSLGVRLVLVFGS 60 Query: 61 RPQIEARLAARGLAPRYHRDLRVTDAPTLECVIDAVGSLRIAIEARLSMDMAASPMQGAR 120 RPQIEARLAARG+ PR+HRDLRVTDAPT+ECVIDAVG LRIA+EARLSMDMAASPMQGAR Sbjct: 61 RPQIEARLAARGIEPRFHRDLRVTDAPTMECVIDAVGQLRIALEARLSMDMAASPMQGAR 120 Query: 121 LRVAGGNLVTARPIGVVEGVDYHHTGEVRRIDRKGIGRLLDERSIVLLSPLGYSPTGEIF 180 LRVAGGN VTARPIGV++GVD HHTGEVRRIDRKGIGRLLDER+IVLLSPLGYSPTGEIF Sbjct: 121 LRVAGGNFVTARPIGVLDGVDLHHTGEVRRIDRKGIGRLLDERTIVLLSPLGYSPTGEIF 180 Query: 181 NLACEDVAMRAAIDLEAEKLILYGAEQGLLDASGKLVRELRPQQVPAHLQRLGNSYQAEL 240 NLACEDVA RAAIDL+A+KL+LYGAE GLLD SGKLVRELRPQQ+PA+++RLG+ YQAEL Sbjct: 181 NLACEDVATRAAIDLQADKLVLYGAEPGLLDESGKLVRELRPQQIPAYVERLGSQYQAEL 240 Query: 241 LDAAAQACRAGVKRSHIVSYTEDGALLSELFTRTGNGTLVAQEQFEQLREAGIEDVGGLI 300 LDAAAQACR GV+RSH+VSY +DGALL+ELFTR G GTLV QEQFEQLREA IEDVGGLI Sbjct: 241 LDAAAQACRGGVRRSHVVSYADDGALLNELFTRDGGGTLVTQEQFEQLREATIEDVGGLI 300 Query: 301 ELIRPLEEQGILVRRSREVLEREIEQFSIVEREGLIIACAALYPIADSEAGELACLAVNP 360 +LI PLEEQGILVRRSREVLERE+EQFSIVER+GLIIACAALYPIADS+AGELACLAVNP Sbjct: 301 DLITPLEEQGILVRRSREVLEREVEQFSIVERDGLIIACAALYPIADSDAGELACLAVNP 360 Query: 361 EYRHGGRGDELLERIEERARGLGLKTLFVLTTRTAHWFRERGFQPSSVERLPAARASLYN 420 EYRHGGRGDELLERIEERAR LGLK L VLTTRTAHWFRERGF+P SV+RLPAARASLYN Sbjct: 361 EYRHGGRGDELLERIEERARKLGLKKLIVLTTRTAHWFRERGFEPISVDRLPAARASLYN 420 Query: 421 FQRNSQVFEKSL 432 FQRNS++FEK L Sbjct: 421 FQRNSKIFEKPL 432 Lambda K H 0.321 0.138 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 744 Number of extensions: 19 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 432 Length of database: 432 Length adjustment: 32 Effective length of query: 400 Effective length of database: 400 Effective search space: 160000 Effective search space used: 160000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 51 (24.3 bits)
Align candidate GFF3930 Psest_4000 (amino-acid N-acetyltransferase)
to HMM TIGR01890 (argA: amino-acid N-acetyltransferase (EC 2.3.1.1))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR01890.hmm # target sequence database: /tmp/gapView.9536.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01890 [M=429] Accession: TIGR01890 Description: N-Ac-Glu-synth: amino-acid N-acetyltransferase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 5.5e-214 696.8 0.0 6.1e-214 696.7 0.0 1.0 1 lcl|FitnessBrowser__psRCH2:GFF3930 Psest_4000 amino-acid N-acetyltr Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__psRCH2:GFF3930 Psest_4000 amino-acid N-acetyltransferase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 696.7 0.0 6.1e-214 6.1e-214 1 429 [] 4 432 .] 4 432 .] 1.00 Alignments for each domain: == domain 1 score: 696.7 bits; conditional E-value: 6.1e-214 TIGR01890 1 fvkwlreaaPyinahrdktlvvglggelvedknlgklvadiallhslGvrlvlvhGarpqieerlakrgrtthyv 75 +v wlr+++Pyin+hrd+t+vv+l+g+++e+ n++++v+d++llhslGvrlvlv G+rpqie rla+rg++++++ lcl|FitnessBrowser__psRCH2:GFF3930 4 YVTWLRDSSPYINSHRDRTFVVMLPGDGLEHANFANIVHDLVLLHSLGVRLVLVFGSRPQIEARLAARGIEPRFH 78 89************************************************************************* PP TIGR01890 76 rGlrvtdeaslelvkeaaGelrlaiearlsmslantpmagsrlsvvsGnfvtarPiGvveGvdyehtGevrkida 150 r lrvtd+ ++e+v++a+G+lr a+earlsm++a++pm+g+rl+v+ GnfvtarPiGv++Gvd++htGevr+id+ lcl|FitnessBrowser__psRCH2:GFF3930 79 RDLRVTDAPTMECVIDAVGQLRIALEARLSMDMAASPMQGARLRVAGGNFVTARPIGVLDGVDLHHTGEVRRIDR 153 *************************************************************************** PP TIGR01890 151 egirrlldersivllsPlgfsvtGeifnlamedvatsvaiklkadklillteedGildadGklvaelsaqevesl 225 +gi rllder ivllsPlg+s+tGeifnla+edvat++ai+l+adkl+l ++e+G+ld++Gklv+el++q++ + lcl|FitnessBrowser__psRCH2:GFF3930 154 KGIGRLLDERTIVLLSPLGYSPTGEIFNLACEDVATRAAIDLQADKLVLYGAEPGLLDESGKLVRELRPQQIPAY 228 *************************************************************************** PP TIGR01890 226 verleeettarllsaavkalrgGvarshlvsyaedGallqelftrdGiGtlvskealesireatiddvggileli 300 verl+++++a+ll+aa++a+rgGv+rsh+vsya dGall+elftrdG Gtlv++e++e++reati+dvgg+++li lcl|FitnessBrowser__psRCH2:GFF3930 229 VERLGSQYQAELLDAAAQACRGGVRRSHVVSYADDGALLNELFTRDGGGTLVTQEQFEQLREATIEDVGGLIDLI 303 *************************************************************************** PP TIGR01890 301 rPleeqGilvrrsrellereieefsviekdGliigcaalypyaeeevgelaclavsPeardggrGerllkhiedr 375 PleeqGilvrrsre+lere+e+fs++e+dGlii+caalyp+a++++gelaclav+Pe+r+ggrG++ll++ie+r lcl|FitnessBrowser__psRCH2:GFF3930 304 TPLEEQGILVRRSREVLEREVEQFSIVERDGLIIACAALYPIADSDAGELACLAVNPEYRHGGRGDELLERIEER 378 *************************************************************************** PP TIGR01890 376 arqvGlkrlfvlttrtehWfrerGfaeasvdelPearrklynyqrrskilvkkl 429 ar++Glk+l vlttrt+hWfrerGf++ svd+lP+ar++lyn+qr+ski++k+l lcl|FitnessBrowser__psRCH2:GFF3930 379 ARKLGLKKLIVLTTRTAHWFRERGFEPISVDRLPAARASLYNFQRNSKIFEKPL 432 ***************************************************987 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (429 nodes) Target sequences: 1 (432 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.02 # Mc/sec: 9.24 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory