Align Aspartate--tRNA(Asp/Asn) ligase; Aspartyl-tRNA synthetase; AspRS; Non-discriminating aspartyl-tRNA synthetase; ND-AspRS; EC 6.1.1.23 (characterized)
to candidate GFF1456 Psest_1493 aspartyl-tRNA synthetase, bacterial type
Query= SwissProt::Q51422 (591 letters) >FitnessBrowser__psRCH2:GFF1456 Length = 591 Score = 1099 bits (2842), Expect = 0.0 Identities = 544/591 (92%), Positives = 569/591 (96%) Query: 1 MMRSHYCGQLNESLDGQEVTLCGWVHRRRDHGGVIFLDVRDREGLAQVVFDPDRAETFAK 60 MMRSHYCGQLNESLDGQE+TLCGWVHRRRDHGGVIFLDVRDREGLAQVVFDPDRAETFA Sbjct: 1 MMRSHYCGQLNESLDGQEITLCGWVHRRRDHGGVIFLDVRDREGLAQVVFDPDRAETFAT 60 Query: 61 ADRVRSEFVVKITGKVRLRPEGARNPNMASGSIEVLGYELEVLNQAETPPFPLDEYSDVG 120 AD+VRSE+VVKITGKVRLRP GA NPN+ASG+IEVLGYEL+VLN AETPPFPL+EYSDVG Sbjct: 61 ADKVRSEYVVKITGKVRLRPAGAVNPNIASGAIEVLGYELDVLNDAETPPFPLNEYSDVG 120 Query: 121 EETRLRYRFIDLRRPEMAAKLKLRARITSSIRRYLDDNGFLDVETPILGRPTPEGARDYL 180 EETRLRYRFIDLRRPEMA KLKLR+RIT+SIRRYLD+NGFLDVETPILGRPTPEGARDYL Sbjct: 121 EETRLRYRFIDLRRPEMAEKLKLRSRITASIRRYLDENGFLDVETPILGRPTPEGARDYL 180 Query: 181 VPSRTYPGHFFALPQSPQLFKQLLMVAGFDRYYQIAKCFRDEDLRADRQPEFTQIDIETS 240 VPSRT+ G+FFALPQSPQLFKQLLMVAGFDRYYQIAKCFRDEDLRADRQPEFTQIDIETS Sbjct: 181 VPSRTHAGNFFALPQSPQLFKQLLMVAGFDRYYQIAKCFRDEDLRADRQPEFTQIDIETS 240 Query: 241 FLDESDIIGITEKMVRQLFKEVLDVEFDEFPHMPFEEAMRRYGSDKPDLRIPLELVDVAD 300 FLDE+DII ITE M+R+LFKEVLD+EF EFPHMPFEEAMRRYGSDKPDLRIPLELVDVAD Sbjct: 241 FLDEADIIDITEGMIRKLFKEVLDLEFGEFPHMPFEEAMRRYGSDKPDLRIPLELVDVAD 300 Query: 301 QLKEVEFKVFSGPANDPKGRVAALRVPGAASMPRSQIDDYTKFVGIYGAKGLAYIKVNER 360 QL VEFKVFSGPANDPKGRVAALRVPGAASMPRSQIDDYTKFVGIYGAKGLAYIKVNER Sbjct: 301 QLNAVEFKVFSGPANDPKGRVAALRVPGAASMPRSQIDDYTKFVGIYGAKGLAYIKVNER 360 Query: 361 AKGVEGLQSPIVKFIPEANLNVILDRVGAVDGDIVFFGADKAKIVCDALGALRIKVGHDL 420 AKGVEGLQSPIVKFIPE NLNVILDRVGAVDGDIVFFGADKAKIV +ALGALRIK+GHDL Sbjct: 361 AKGVEGLQSPIVKFIPEDNLNVILDRVGAVDGDIVFFGADKAKIVSEALGALRIKIGHDL 420 Query: 421 KLLTREWAPMWVVDFPMFEENDDGSLSALHHPFTSPKCTPAELEANPGAALSRAYDMVLN 480 KLLT +WAP+WVVDFPMFEENDDGSLSALHHPFT+PKCTP ELEANP AALSRAYDMVLN Sbjct: 421 KLLTCDWAPLWVVDFPMFEENDDGSLSALHHPFTAPKCTPQELEANPAAALSRAYDMVLN 480 Query: 481 GTELGGGSIRIHDKSMQQAVFRVLGIDEAEQEEKFGFLLDALKYGAPPHGGLAFGLDRLV 540 GTELGGGSIRIH K MQQ VFR+LGIDEAEQEEKFGFLLDALK+GAPPHGGLAFGLDRLV Sbjct: 481 GTELGGGSIRIHRKEMQQTVFRILGIDEAEQEEKFGFLLDALKFGAPPHGGLAFGLDRLV 540 Query: 541 MLMTGASSIREVIAFPKTQSAGDVMTQAPGSVDGKALRELHIRLREQPKAE 591 MLMTGA SIREVIAFPKTQSA DVMTQAPG+VD KALR+LHIRLREQPKAE Sbjct: 541 MLMTGAQSIREVIAFPKTQSAADVMTQAPGAVDNKALRDLHIRLREQPKAE 591 Lambda K H 0.321 0.140 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1366 Number of extensions: 36 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 591 Length of database: 591 Length adjustment: 37 Effective length of query: 554 Effective length of database: 554 Effective search space: 306916 Effective search space used: 306916 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory