Alignments for a candidate for hisH in Pseudomonas stutzeri RCH2

GapMind for Amino acid biosynthesis

Alignments for a candidate for hisH in Pseudomonas stutzeri RCH2

Align IGP synthase, amidotransferase subunit (EC 4.3.2.10) (characterized)
to candidate GFF153 Psest_0153 imidazole glycerol phosphate synthase, glutamine amidotransferase subunit

Query= reanno::HerbieS:HSERO_RS20325
         (212 letters)



>FitnessBrowser__psRCH2:GFF153
          Length = 212

 Score =  196 bits (499), Expect = 2e-55
 Identities = 105/211 (49%), Positives = 127/211 (60%), Gaps = 4/211 (1%)

Query: 1   MNKIVVVDYGMGNLRSVAQALRHVAPEADVRISGEVADIRAADRVVLPGQGAMPDCMRSL 60
           M  + V+DYGMGNL SVA+AL HV     V I+ +   IR ADRVV PG GA+ DCM  +
Sbjct: 1   MQTVAVIDYGMGNLHSVAKALEHVGA-GRVLITSDAQVIREADRVVFPGVGAIRDCMAEI 59

Query: 61  RESGVQDAVIEASRTKPLFGVCVGEQMLFDWSEEGD-TPGLGLLPGKVVRFDLEGMRQDD 119
           R  G  + V E S+ +P  G+CVG Q L + SEE +    +GL PG+V  F  +    +D
Sbjct: 60  RRLGFDELVREVSQDRPFLGICVGMQALMERSEENEGVDCIGLFPGQVRFFGKD--LHED 117

Query: 120 GSLFKVPQMGWNHVHQTSRHPLWEGIADNAFFYFVHSYYAVPAESAHVVGQTPYGRDFAC 179
           G   KVP MGWN V Q   HPLW  I DN  FYFVHSYY        VVG+  YG DFA 
Sbjct: 118 GEHLKVPHMGWNEVKQVVDHPLWHAIPDNGRFYFVHSYYIEAGNPRQVVGRGHYGNDFAA 177

Query: 180 AVARDNIFATQFHPEKSASAGLQLYRNFVHW 210
           A+A  + FA QFHPEKS + GLQL +NF  W
Sbjct: 178 ALADGSRFAVQFHPEKSHTHGLQLLQNFAAW 208


Lambda     K      H
   0.322    0.137    0.431 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 172
Number of extensions: 6
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 212
Length of database: 212
Length adjustment: 21
Effective length of query: 191
Effective length of database: 191
Effective search space:    36481
Effective search space used:    36481
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 45 (21.9 bits)

Align candidate GFF153 Psest_0153 (imidazole glycerol phosphate synthase, glutamine amidotransferase subunit)
to HMM TIGR01855 (hisH: imidazole glycerol phosphate synthase, glutamine amidotransferase subunit (EC 2.4.2.-))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01855.hmm
# target sequence database:        /tmp/gapView.7304.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01855  [M=198]
Accession:   TIGR01855
Description: IMP_synth_hisH: imidazole glycerol phosphate synthase, glutamine amidotransferase subunit
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                          Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                          -----------
    8.7e-70  220.6   0.0    9.9e-70  220.4   0.0    1.0  1  lcl|FitnessBrowser__psRCH2:GFF153  Psest_0153 imidazole glycerol ph


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__psRCH2:GFF153  Psest_0153 imidazole glycerol phosphate synthase, glutamine amidotransferase subun
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  220.4   0.0   9.9e-70   9.9e-70       1     197 [.       4     207 ..       4     208 .. 0.95

  Alignments for each domain:
  == domain 1  score: 220.4 bits;  conditional E-value: 9.9e-70
                          TIGR01855   1 ivvidygvgNlksvkkalervgaes.evvkdskelekadklvlPGVGafkeamkklrelelellaekvvkkkkpvl 75 
                                        ++vidyg+gNl+sv+kale+vga    +++d++ +++ad++v+PGVGa +++m+++r+l+ + l  ++v++++p+l
  lcl|FitnessBrowser__psRCH2:GFF153   4 VAVIDYGMGNLHSVAKALEHVGAGRvLITSDAQVIREADRVVFPGVGAIRDCMAEIRRLGFDEL-VREVSQDRPFL 78 
                                        79********************9651578899****************************8888.46677888*** PP

                          TIGR01855  76 giClGmQllfekseEgkevkglglikgkvkkleaek.......kvPhiGWnevevvkesellkgleeearvYfvHs 144
                                        giC+GmQ l+e+seE++ v+++gl++g+v+ + ++        kvPh+GWnev+ v +++l + + ++ r+YfvHs
  lcl|FitnessBrowser__psRCH2:GFF153  79 GICVGMQALMERSEENEGVDCIGLFPGQVRFFGKDLhedgehlKVPHMGWNEVKQVVDHPLWHAIPDNGRFYFVHS 154
                                        *****************************99988777788999********************************* PP

                          TIGR01855 145 YaveleeeeavlakadygekfvaavekdnivgvQFHPEkSgktGlkllknfle 197
                                        Y++e  +  +v+   +yg++f aa++++  ++vQFHPEkS++ Gl+ll+nf++
  lcl|FitnessBrowser__psRCH2:GFF153 155 YYIEAGNPRQVVGRGHYGNDFAAALADGSRFAVQFHPEKSHTHGLQLLQNFAA 207
                                        ***************************************************86 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (198 nodes)
Target sequences:                          1  (212 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.01s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 6.95
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis