Align succinylornithine transaminase (EC 2.6.1.81) (characterized)
to candidate GFF2666 Psest_2719 acetylornithine and succinylornithine aminotransferases/succinylornithine transaminase family
Query= BRENDA::O30508 (406 letters) >FitnessBrowser__psRCH2:GFF2666 Length = 406 Score = 666 bits (1719), Expect = 0.0 Identities = 322/405 (79%), Positives = 362/405 (89%) Query: 1 MSAPHAQVERADFDRYMVPNYAPAAFIPVRGEGSRVWDQSGRELIDFAGGIAVTSLGHAH 60 MSAPH VERADFD+ +VP +APAAF+PVRG GSRVWDQSGREL+DFAGGIAV +LGHAH Sbjct: 1 MSAPHTPVERADFDQVIVPTFAPAAFVPVRGLGSRVWDQSGRELVDFAGGIAVNALGHAH 60 Query: 61 PALVKALTEQAQRIWHVSNVFTNEPALRLARKLVDATFAERVFLANSGAEANEAAFKLAR 120 PA+V ALTEQA ++WH+SN++TNEPALRLA+KLV ATFA+R F NSGAEANEAAFKLAR Sbjct: 61 PAMVAALTEQAGKLWHISNIYTNEPALRLAKKLVAATFADRAFFCNSGAEANEAAFKLAR 120 Query: 121 RYANDVYGPQKYEIIAASNSFHGRTLFTVNVGGQPKYSDGFGPKFEGITHVPYNDLEALK 180 RYA+DVYGPQK+EII+A NSFHGRTLFTV VGGQ KYSDGFGPK EGITHVPYNDLEALK Sbjct: 121 RYAHDVYGPQKFEIISALNSFHGRTLFTVTVGGQSKYSDGFGPKIEGITHVPYNDLEALK 180 Query: 181 AAISDKTCAVVLEPIQGEGGVLPAQQAYLEGARKLCDEHNALLVFDEVQSGMGRVGELFA 240 AAISDKTCAVVLEPIQGE G+LP +QAYLEGAR+LC+EHNALL+FDEVQ+GMGR GELFA Sbjct: 181 AAISDKTCAVVLEPIQGESGILPGEQAYLEGARQLCNEHNALLIFDEVQTGMGRTGELFA 240 Query: 241 YMHYGVVPDILSSAKSLGGGFPIGAMLTTGEIAKHLSVGTHGTTYGGNPLASAVAEAALD 300 YMHYG+ PDIL++AKSLGGGFPIGAMLTT EIA HLSVGTHGTTYGGNPLA AVAEA +D Sbjct: 241 YMHYGITPDILTNAKSLGGGFPIGAMLTTNEIAAHLSVGTHGTTYGGNPLACAVAEAVVD 300 Query: 301 VINTPEVLDGVKAKHERFKSRLQKIGQEYGIFDEIRGMGLLIGAALTDEWKGKARDVLNA 360 ++NTPEVL+GVKAKHERFK+RL +IG+ YG+F +RG GLLIG L+D WKGKA A Sbjct: 301 IVNTPEVLEGVKAKHERFKARLTQIGERYGVFSLVRGRGLLIGCVLSDAWKGKAGAFCAA 360 Query: 361 AEKEAVMVLQASPDVVRFAPSLVIDDAEIDEGLERFERAVAKLVR 405 AEKEA+MVLQA PDVVR APSLVID A+IDEGL+R ERAVA L + Sbjct: 361 AEKEALMVLQAGPDVVRLAPSLVIDQADIDEGLDRLERAVAALTQ 405 Lambda K H 0.318 0.135 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 623 Number of extensions: 14 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 406 Length of database: 406 Length adjustment: 31 Effective length of query: 375 Effective length of database: 375 Effective search space: 140625 Effective search space used: 140625 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 50 (23.9 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory