GapMind for Amino acid biosynthesis

 

Protein PfGW456L13_4295 in Pseudomonas fluorescens GW456-L13

Annotation: FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_4295

Length: 591 amino acids

Source: pseudo13_GW456_L13 in FitnessBrowser

Candidate for 3 steps in Amino acid biosynthesis

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-isoleucine biosynthesis ilvI lo Probable acetolactate synthase large subunit; AHAS; EC 2.2.1.6; Acetohydroxy-acid synthase large subunit; ALS (uncharacterized) 33% 98% 336.7 Glyoxylate carboligase (EC 4.1.1.47) 77% 943.0
L-leucine biosynthesis ilvI lo Probable acetolactate synthase large subunit; AHAS; EC 2.2.1.6; Acetohydroxy-acid synthase large subunit; ALS (uncharacterized) 33% 98% 336.7 Glyoxylate carboligase (EC 4.1.1.47) 77% 943.0
L-valine biosynthesis ilvI lo Probable acetolactate synthase large subunit; AHAS; EC 2.2.1.6; Acetohydroxy-acid synthase large subunit; ALS (uncharacterized) 33% 98% 336.7 Glyoxylate carboligase (EC 4.1.1.47) 77% 943.0

Sequence Analysis Tools

View PfGW456L13_4295 at FitnessBrowser

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MSKMRAIEAAVLVMRREGVDTAFGIPGAAINPLYSALQKVGGIDHVLARHVEGASHMAEG
YTRTKAGNIGVCIGTSGPAGTDMVTGLYSASADSIPILCITGQAPRARMHKEDFQAVDIT
TIVKPVTKWSTTVMEPGQVPYAFQKAFYEMRSGRPGPVLIDLPFDVQMAEIEFDIDAYQP
LPLAKPSATRVQVEKALAMLDQAERPLLVAGGGIINADAADLLVEFAELTGIPVIPTLMG
WGAIPDDHPLMVGMVGLQTSHRYGNATMLKSDVVLGVGNRWANRHTGSVDVYTEGRKFIH
VDIEPTQIGRVFTPDLGIVSDAAAALTVFIEVAREWQAAGKLKNRSAWLQDCQQRKASLQ
RKTHFDNVPVKPQRVYEEMNQVFGKDTCYVSTIGLSQIAGAQFLHVYKPRHWINCGQAGP
LGWTIPAALGVVKADPNRKVVALSGDYDFQFMIEELAVGAQFKLPYIHVVVNNSYLGLIR
QAQRGFDMDYCVQLSFDNLNAPELNGYGVDHVAVAEGLGCKALRVFEPAQIQPALRKAQE
LIEEFKVPVIVEIILERVTNISMGTEINAVNEFEDLALVGDDAPTAISLLD

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory