Align Ornithine carbamoyltransferase, catabolic; OTCase; EC 2.1.3.3 (characterized)
to candidate PfGW456L13_1873 Ornithine carbamoyltransferase (EC 2.1.3.3)
Query= SwissProt::P08308 (336 letters) >FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1873 Length = 336 Score = 617 bits (1591), Expect = 0.0 Identities = 300/336 (89%), Positives = 320/336 (95%) Query: 1 MAFNMHNRNLLSLMHHSTRELRYLLDLSRDLKRAKYTGTEQQHLKRKNIALIFEKTSTRT 60 MAFN+HNRNLLSL HH+ RELRYLLDLSRDLKRAKYTGTEQQHLK NIALIFEKTSTRT Sbjct: 1 MAFNIHNRNLLSLEHHTPRELRYLLDLSRDLKRAKYTGTEQQHLKGNNIALIFEKTSTRT 60 Query: 61 RCAFEVAAYDQGANVTYIDPNSSQIGHKESMKDTARVLGRMYDAIEYRGFKQEIVEELAK 120 RCAFEVAAYDQGANVTYIDPNSSQIGHKESMKDTARVLGRMYDAIEYRGFKQEIVEELAK Sbjct: 61 RCAFEVAAYDQGANVTYIDPNSSQIGHKESMKDTARVLGRMYDAIEYRGFKQEIVEELAK 120 Query: 121 FAGVPVFNGLTDEYHPTQMLADVLTMREHSDKPLHDISYAYLGDARNNMGNSLLLIGAKL 180 FAGVPVFNGLTDEYHPTQM+ADVLTMREH+DKP+H+ISYAYLGDARNNMGNSLLLIGAKL Sbjct: 121 FAGVPVFNGLTDEYHPTQMIADVLTMREHADKPIHEISYAYLGDARNNMGNSLLLIGAKL 180 Query: 181 GMDVRIAAPKALWPHDEFVAQCKKFAEESGAKLTLTEDPKEAVKGVDFVHTDVWVSMGEP 240 GMDVRI APKALWP D+ VA+CK++ EESGA++TLTEDPK AVKGVDF+HTDVWVSMGEP Sbjct: 181 GMDVRICAPKALWPADDLVARCKQYGEESGARITLTEDPKAAVKGVDFIHTDVWVSMGEP 240 Query: 241 VEAWGERIKELLPYQVNMEIMKATGNPRAKFMHCLPAFHNSETKVGKQIAEQYPNLANGI 300 VEAW ERI++LLPYQVN E+MKATGNPR KFMHCLPAFHN +TKVGKQIAEQYP L+NGI Sbjct: 241 VEAWAERIQQLLPYQVNAELMKATGNPRTKFMHCLPAFHNCDTKVGKQIAEQYPYLSNGI 300 Query: 301 EVTEDVFESPYNIAFEQAENRMHTIKAILVSTLADI 336 EVT+DVFESP IAFEQAENRMHTIKAILVSTLAD+ Sbjct: 301 EVTDDVFESPACIAFEQAENRMHTIKAILVSTLADL 336 Lambda K H 0.318 0.133 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 481 Number of extensions: 6 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 336 Length of database: 336 Length adjustment: 28 Effective length of query: 308 Effective length of database: 308 Effective search space: 94864 Effective search space used: 94864 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 49 (23.5 bits)
Align candidate PfGW456L13_1873 (Ornithine carbamoyltransferase (EC 2.1.3.3))
to HMM TIGR00658 (argF: ornithine carbamoyltransferase (EC 2.1.3.3))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00658.hmm # target sequence database: /tmp/gapView.29706.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00658 [M=304] Accession: TIGR00658 Description: orni_carb_tr: ornithine carbamoyltransferase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 4.6e-137 442.2 0.0 5.2e-137 442.0 0.0 1.0 1 lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1873 Ornithine carbamoyltransferase ( Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1873 Ornithine carbamoyltransferase (EC 2.1.3.3) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 442.0 0.0 5.2e-137 5.2e-137 1 303 [. 8 333 .. 8 334 .. 0.99 Alignments for each domain: == domain 1 score: 442.0 bits; conditional E-value: 5.2e-137 TIGR00658 1 rhllslldlseeelkellelakklkkekkkgkeekklkgktlaliFekrstRtRv 55 r+llsl +++++el++ll+l+++lk++k++g+e+++lkg+++aliFek+stRtR+ lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1873 8 RNLLSLEHHTPRELRYLLDLSRDLKRAKYTGTEQQHLKGNNIALIFEKTSTRTRC 62 78***************************************************** PP TIGR00658 56 sfevaayelGaqvlylnkeelqlgrkesikDtarvlsryvdaivvRvykhedvee 110 +fevaay++Ga+v+y++++++q+g+kes+kDtarvl+r++dai +R++k+e+vee lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1873 63 AFEVAAYDQGANVTYIDPNSSQIGHKESMKDTARVLGRMYDAIEYRGFKQEIVEE 117 ******************************************************* PP TIGR00658 111 lakyasvPvingLtdlehPcqilaDlltikeklg.klkevklvyvGDa.nnvans 163 lak+a+vPv+ngLtd+ hP+q++aD+lt++e+ + ++e++++y+GDa nn++ns lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1873 118 LAKFAGVPVFNGLTDEYHPTQMIADVLTMREHADkPIHEISYAYLGDArNNMGNS 172 **********************************99******************* PP TIGR00658 164 lllaaaklGldvvvatPeglepeaeivkkakkiakenggkleltedpkkavkdad 218 lll++aklG+dv++++P++l+p ++v ++k+ +e+g++++ltedpk avk++d lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1873 173 LLLIGAKLGMDVRICAPKALWPADDLVARCKQYGEESGARITLTEDPKAAVKGVD 227 ******************************************************* PP TIGR00658 219 viytDvwvsmGe.eekkeerlkllkpyqvneellela.kpevkflhCLPavr... 268 +i+tDvwvsmGe e+++er+++l pyqvn el++ + +p +kf+hCLPa++ lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1873 228 FIHTDVWVSMGEpVEAWAERIQQLLPYQVNAELMKATgNPRTKFMHCLPAFHncd 282 ************9999*************************************** PP TIGR00658 269 ................GeevtdevlegeasivfdeaenRlhaqkavlkall 303 G evtd+v+e++a i f++aenR+h++ka+l+ +l lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1873 283 tkvgkqiaeqypylsnGIEVTDDVFESPACIAFEQAENRMHTIKAILVSTL 333 ***********************************************9887 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (304 nodes) Target sequences: 1 (336 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 9.91 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory