Alignments for a candidate for aroC in Pseudomonas fluorescens GW456-L13

GapMind for Amino acid biosynthesis

Alignments for a candidate for aroC in Pseudomonas fluorescens GW456-L13

Align Chorismate synthase; CS; 5-enolpyruvylshikimate-3-phosphate phospholyase; EPSP phospholyase; EC 4.2.3.5 (characterized)
to candidate PfGW456L13_4165 Chorismate synthase (EC 4.2.3.5)

Query= SwissProt::P12008
         (361 letters)



>FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_4165
          Length = 363

 Score =  513 bits (1322), Expect = e-150
 Identities = 257/359 (71%), Positives = 299/359 (83%), Gaps = 1/359 (0%)

Query: 1   MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHDLDRRRPGTSRYTTQRR 60
           M+GNT G+LF VTT GESHG AL  IVDG PPG+ ++  DLQ DLDRR+PGTSR+TTQR+
Sbjct: 1   MSGNTYGKLFTVTTAGESHGPALVAIVDGCPPGLEISLEDLQRDLDRRKPGTSRHTTQRQ 60

Query: 61  EPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVFRPGHADYTYEQKYGLRDY 120
           E D+V+ILSGVFEG TTG +IGLLI NTDQ+S+DYSAIKD+FRP HADYTY  KYG RDY
Sbjct: 61  EADEVEILSGVFEGRTTGCAIGLLIRNTDQKSKDYSAIKDLFRPAHADYTYHHKYGERDY 120

Query: 121 RGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQMGDIPLDIKDWSQVEQNPFFC 180
           RGGGRSSARETAMRVAAGAIAKKYLA + GI IRG ++Q+G I +  K W  VEQN FF 
Sbjct: 121 RGGGRSSARETAMRVAAGAIAKKYLASQ-GIVIRGYMSQLGPIEIPFKTWDSVEQNAFFS 179

Query: 181 PDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGLGEPVFDRLDADIAHALMSINAV 240
           PDPDK+  L+  M  L+++ DS+GAK+TVVA GV  GLGEP+FDRLDA++AHALMSINAV
Sbjct: 180 PDPDKVPELEAYMDQLRRDQDSVGAKITVVAEGVMPGLGEPIFDRLDAELAHALMSINAV 239

Query: 241 KGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGILGGISSGQQIIAHMALKPTSSITV 300
           KGVEIG GF  VA RG+++RDE+T +GF SN+AGGILGGISSGQ I+AH+ALKPTSSIT 
Sbjct: 240 KGVEIGAGFASVAQRGTEHRDEMTPEGFLSNNAGGILGGISSGQPIVAHLALKPTSSITT 299

Query: 301 PGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEAMLAIVLMDHLLRQRAQNADVKTDIP 359
           PGR+I+  G  VE+ITKGRHDPCVGIRA PIAEAM+AIVLMDHLLR R QNADV+   P
Sbjct: 300 PGRSIDIHGNPVEVITKGRHDPCVGIRATPIAEAMMAIVLMDHLLRHRGQNADVRVSTP 358


Lambda     K      H
   0.319    0.138    0.404 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 464
Number of extensions: 16
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 361
Length of database: 363
Length adjustment: 29
Effective length of query: 332
Effective length of database: 334
Effective search space:   110888
Effective search space used:   110888
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 49 (23.5 bits)

Align candidate PfGW456L13_4165 (Chorismate synthase (EC 4.2.3.5))
to HMM TIGR00033 (aroC: chorismate synthase (EC 4.2.3.5))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00033.hmm
# target sequence database:        /tmp/gapView.3675.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00033  [M=351]
Accession:   TIGR00033
Description: aroC: chorismate synthase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                               Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                               -----------
   4.8e-142  459.0   0.1   5.5e-142  458.8   0.1    1.0  1  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_4165  Chorismate synthase (EC 4.2.3.5)


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_4165  Chorismate synthase (EC 4.2.3.5)
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  458.8   0.1  5.5e-142  5.5e-142       1     350 [.      10     349 ..      10     350 .. 0.98

  Alignments for each domain:
  == domain 1  score: 458.8 bits;  conditional E-value: 5.5e-142
                                               TIGR00033   1 lrlttfGeSHgkalgaiidGlPaglelteediqkelkrRrpgqsrltrmrkEeDe 55 
                                                             +++tt+GeSHg+al+ai+dG+P+gle++ ed+q++l+rR+pg+sr+t++r+E+De
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_4165  10 FTVTTAGESHGPALVAIVDGCPPGLEISLEDLQRDLDRRKPGTSRHTTQRQEADE 64 
                                                             789**************************************************** PP

                                               TIGR00033  56 veilsGvfeGkTtGaPiallikNkdvrskdyedikelpRPgHadytylkKYgikd 110
                                                             veilsGvfeG+TtG  i lli+N+d++skdy+ ik+l+RP+Hadyty +KYg +d
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_4165  65 VEILSGVFEGRTTGCAIGLLIRNTDQKSKDYSAIKDLFRPAHADYTYHHKYGERD 119
                                                             ******************************************************* PP

                                               TIGR00033 111 regggrsSaReTaarvaaGavakklLketagieivayvvklgeveleeesakeis 165
                                                             ++gggrsSaReTa+rvaaGa+akk+L++ +gi i +y+++lg +e+++++ +   
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_4165 120 YRGGGRSSARETAMRVAAGAIAKKYLAS-QGIVIRGYMSQLGPIEIPFKTWD--- 170
                                                             ****************************.99****************86665... PP

                                               TIGR00033 166 kerldkspvrcpdaeaekemeeeidkakkdgdsvGgvvevvvsnvpvglGeplfd 220
                                                               +++++ ++ pd+++  e+e+++d++++d+dsvG++++vv+++v  glGep+fd
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_4165 171 --SVEQNAFFSPDPDKVPELEAYMDQLRRDQDSVGAKITVVAEGVMPGLGEPIFD 223
                                                             ..6999************************************************* PP

                                               TIGR00033 221 kldaelasallsinAvKgveiGdGFeaasvrGseanDelvleddkirrktnnsGG 275
                                                             +ldaela+al+sinAvKgveiG+GF+++ +rG e+ De++ e     + +nn GG
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_4165 224 RLDAELAHALMSINAVKGVEIGAGFASVAQRGTEHRDEMTPE----GFLSNNAGG 274
                                                             *************************************88654....69******* PP

                                               TIGR00033 276 ieGGitnGedirvriavKpiptikkplktvdletkekakatkgRhDpcvvpravp 330
                                                             i+GGi+ G++i+ + a+Kp+++i++p +++d+++++   +tkgRhDpcv +ra+p
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_4165 275 ILGGISSGQPIVAHLALKPTSSITTPGRSIDIHGNPVEVITKGRHDPCVGIRATP 329
                                                             ******************************************************* PP

                                               TIGR00033 331 vvEamvalvladallekras 350
                                                             ++Eam+a+vl+d+ll++r++
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_4165 330 IAEAMMAIVLMDHLLRHRGQ 349
                                                             *****************987 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (351 nodes)
Target sequences:                          1  (363 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.01
# Mc/sec: 6.70
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis