Alignments for a candidate for asp-kinase in Pseudomonas fluorescens GW456-L13

GapMind for Amino acid biosynthesis

Alignments for a candidate for asp-kinase in Pseudomonas fluorescens GW456-L13

Align Aspartate kinase; Aspartokinase; EC 2.7.2.4 (characterized)
to candidate PfGW456L13_1981 Aspartokinase (EC 2.7.2.4)

Query= SwissProt::C3JXY0
         (413 letters)



>FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1981
          Length = 413

 Score =  745 bits (1924), Expect = 0.0
 Identities = 390/413 (94%), Positives = 401/413 (97%)

Query: 1   MALIVQKFGGTSVGSVERIEQVADKVKKFRDAGDDLVVVLSAMSGETNRLIDLAKAISGD 60
           MALIVQKFGGTSVG+VERIEQVADKVKKFRDAGDDLVVVLSAMSGETNRLIDLAK ISGD
Sbjct: 1   MALIVQKFGGTSVGTVERIEQVADKVKKFRDAGDDLVVVLSAMSGETNRLIDLAKQISGD 60

Query: 61  QQPLPRELDVIVSTGEQVTIALLAMALNKRGVPAVSYTGSQVRILTDSAHTKARILQIDD 120
            QP+PRELDVIVSTGEQVTIALLAMAL KRGVPAVSYTG+QVRILTDSAH KARILQIDD
Sbjct: 61  GQPVPRELDVIVSTGEQVTIALLAMALIKRGVPAVSYTGNQVRILTDSAHNKARILQIDD 120

Query: 121 QKIRTDLKAGRVVVVAGFQGVDEQGNITTLGRGGSDTTGVALAAALKADECQIYTDVDGV 180
           QKIR DLKAGRVVVVAGFQGVDE GNITTLGRGGSDTTGVALAAALKADECQIYTDVDGV
Sbjct: 121 QKIRGDLKAGRVVVVAGFQGVDEHGNITTLGRGGSDTTGVALAAALKADECQIYTDVDGV 180

Query: 181 YTTDPRVVSVAQRLDKITFEEMLEMASLGSKVLQIRAVEFAGKYNVPLRVLHSFKEGPGT 240
           YTTDPRVVSVAQRLDKITFEEMLEMASLGSKVLQIRAVEFAGKYNVPLRVLHSFKEGPGT
Sbjct: 181 YTTDPRVVSVAQRLDKITFEEMLEMASLGSKVLQIRAVEFAGKYNVPLRVLHSFKEGPGT 240

Query: 241 LITIDEEESMEQPIISGIAFNRDEAKLTIRGVPDTPGVAFKILGPISGANIEVDMIVQNV 300
           LITIDEEE+MEQPIISGIAFNRDEAKLTIRGVPD PGVAFKILGPIS ANIEVDMIVQNV
Sbjct: 241 LITIDEEETMEQPIISGIAFNRDEAKLTIRGVPDIPGVAFKILGPISAANIEVDMIVQNV 300

Query: 301 SHDNTTDFTFTVHRNEYDAAERILQNTAKEIGAREVVGDTKIAKVSIVGVGMRSHAGVAS 360
           +HDNTTDFTFTVHRN+Y AA+ +L+ TA E+GAREVVGDTKIAKVSIVGVGMRSHAGVAS
Sbjct: 301 AHDNTTDFTFTVHRNDYQAAQAVLEKTAGELGAREVVGDTKIAKVSIVGVGMRSHAGVAS 360

Query: 361 RMFEALAKESINIQMISTSEIKVSVVIEEKYLELAVRALHTAFELDAPARQGE 413
           RMFE+LAKESINIQMISTSEIKVSVVIEEKYLELAVRALHTAFELDAPARQGE
Sbjct: 361 RMFESLAKESINIQMISTSEIKVSVVIEEKYLELAVRALHTAFELDAPARQGE 413


Lambda     K      H
   0.316    0.133    0.357 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 698
Number of extensions: 23
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 413
Length of database: 413
Length adjustment: 31
Effective length of query: 382
Effective length of database: 382
Effective search space:   145924
Effective search space used:   145924
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 50 (23.9 bits)

Align candidate PfGW456L13_1981 (Aspartokinase (EC 2.7.2.4))
to HMM TIGR00656 (aspartate kinase, monofunctional class (EC 2.7.2.4))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00656.hmm
# target sequence database:        /tmp/gapView.26523.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00656  [M=407]
Accession:   TIGR00656
Description: asp_kin_monofn: aspartate kinase, monofunctional class
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                               Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                               -----------
   2.7e-133  430.8  13.4     3e-133  430.7  13.4    1.0  1  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1981  Aspartokinase (EC 2.7.2.4)


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1981  Aspartokinase (EC 2.7.2.4)
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  430.7  13.4    3e-133    3e-133       1     406 [.       1     405 [.       1     406 [. 0.97

  Alignments for each domain:
  == domain 1  score: 430.7 bits;  conditional E-value: 3e-133
                                               TIGR00656   1 veliVqKFGGtsvgsserikkaakivlkelkegkkvvVVvSAmskvtdelvelae 55 
                                                             + liVqKFGGtsvg +eri+++a++v+k    g++ vVV+SAms++t++l++la+
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1981   1 MALIVQKFGGTSVGTVERIEQVADKVKKFRDAGDDLVVVLSAMSGETNRLIDLAK 55 
                                                             579***************************************************5 PP

                                               TIGR00656  56 llklleaisdeisprerdelvsvGEllssallssalrelgvkaealdgkeagilT 110
                                                                 + +   ++ pre d +vs+GE++++all++al ++gv a++++g++  ilT
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1981  56 ----QISGDGQPVPRELDVIVSTGEQVTIALLAMALIKRGVPAVSYTGNQVRILT 106
                                                             ....345567899****************************************** PP

                                               TIGR00656 111 ddefgnAkikelateerLlelLeegiivvvaGFiGateeGeiTtLGRGGSDltAa 165
                                                             d+ +++A+i +++  +++   L+ g +vvvaGF+G +e+G+iTtLGRGGSD+t +
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1981 107 DSAHNKARILQIDD-QKIRGDLKAGRVVVVAGFQGVDEHGNITTLGRGGSDTTGV 160
                                                             *************9.**************************************** PP

                                               TIGR00656 166 llaaalkAdrveiyTDVeGvyttDPrvveeakkidkisyeEalelAtlGakvlhp 220
                                                             +laaalkAd+++iyTDV+GvyttDPrvv+ a+++dki++eE+le+A+lG kvl+ 
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1981 161 ALAAALKADECQIYTDVDGVYTTDPRVVSVAQRLDKITFEEMLEMASLGSKVLQI 215
                                                             ******************************************************* PP

                                               TIGR00656 221 ralelaveakvpilvrsskekeegTlitn....kkensslvkaialeknvarltv 271
                                                             ra+e+a +++vp++v +s+++  gTlit     ++e++ ++++ia++++ a+lt+
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1981 216 RAVEFAGKYNVPLRVLHSFKEGPGTLITIdeeeTMEQP-IISGIAFNRDEAKLTI 269
                                                             ***************************98665556666.**************** PP

                                               TIGR00656 272 egegmlgkrgilaeifkaLaeeeinvdlisqtese...tsislvvdeedvdeakk 323
                                                             +  g+ + +g++ +i + ++ ++i vd+i+q  ++   t+ +++v+++d ++a++
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1981 270 R--GVPDIPGVAFKILGPISAANIEVDMIVQNVAHdntTDFTFTVHRNDYQAAQA 322
                                                             *..9***************************99988889**************** PP

                                               TIGR00656 324 aLkeesgaaelesleveedlavvsivgaglveapGvaseifkaleekninilmis 378
                                                             +L+++ g+++ +++  + ++a+vsivg+g++++ Gvas++f+ l+++ ini mis
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1981 323 VLEKTAGELGAREVVGDTKIAKVSIVGVGMRSHAGVASRMFESLAKESINIQMIS 377
                                                             ******************************************************* PP

                                               TIGR00656 379 ssetkisvlvdekdaekavrklheklee 406
                                                             +se+k+sv+++ek++e avr+lh ++e+
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1981 378 TSEIKVSVVIEEKYLELAVRALHTAFEL 405
                                                             *************************997 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (407 nodes)
Target sequences:                          1  (413 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.01
# Mc/sec: 10.78
//
[ok]

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis