Align 2-isopropylmalate synthase; Alpha-IPM synthase; Alpha-isopropylmalate synthase; EC 2.3.3.13 (characterized)
to candidate PfGW456L13_4854 2-isopropylmalate synthase (EC 2.3.3.13)
Query= SwissProt::P42455 (616 letters) >FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_4854 Length = 559 Score = 608 bits (1567), Expect = e-178 Identities = 317/572 (55%), Positives = 399/572 (69%), Gaps = 31/572 (5%) Query: 37 PVNRYMPFEVEVEDISLPDRTWPDKKITVAPQWCAVDLRDGNQALIDPMSPERKRRMFEL 96 P ++Y F I +PDRTWP K IT AP WC+ DLRDGNQ+LI+PM +K R ++ Sbjct: 7 PSSKYRAFPT----IDIPDRTWPSKTITAAPIWCSSDLRDGNQSLIEPMDAVKKLRFWKT 62 Query: 97 LVQMGFKEIEVGFPSASQTDFDFVREIIEKGMIPDDVTIQVLVQAREHLIRRTFEACEGA 156 LVQ+G KEIE FP+ASQTDFDFVR +IE IPDD TIQVL Q RE LI RTFE+ GA Sbjct: 63 LVQVGVKEIEASFPAASQTDFDFVRTLIEGNHIPDDTTIQVLTQGREDLIERTFESLRGA 122 Query: 157 KNVIVHFYNSTSILQRNVVFRMDKVQVKKLATDAAELIKTIAQDYPDTNWRWQYSPESFT 216 K IVH YN+TS R +VF DK VK +A +AA+L A PDT W ++YSPE+F+ Sbjct: 123 KKAIVHLYNATSPSFRRIVFNQDKDGVKAIAVNAAKLFVKYAAMQPDTEWTFEYSPETFS 182 Query: 217 GTEVEYAKEVVDAVVEVMDPTPENPMIINLPSTVEMITPNVYADSIEWMHRNLNRRDSII 276 TE+E+AKEV DAV+EV +PTPE+ MI+NLP+TVE TPN+YAD IEW R++NRRDS+I Sbjct: 183 ATEMEFAKEVCDAVIEVWNPTPEHKMILNLPATVECATPNIYADQIEWFGRHINRRDSVI 242 Query: 277 LSLHPHNDRGTGVGAAELGYMAGADRIEGCLFGNGERTGNVCLVTLALNMLTQGVDPQLD 336 +SLH HNDRGTGV A ELG MAGADR+EGCLFGNGERTGNV LVT+ALN+ TQGV+P+LD Sbjct: 243 ISLHTHNDRGTGVAATELGLMAGADRVEGCLFGNGERTGNVDLVTVALNLYTQGVNPELD 302 Query: 337 FTDIRQIRSTVEYCNQLRVPERHPYGGDLVFTAFSGSHQDAVNKGLDAMAAKVQPGASST 396 F+DI +R VE CNQ++V RHPY GDLV TAFSGSHQDA+ KG Sbjct: 303 FSDIDGVRKVVEECNQIQVHPRHPYVGDLVHTAFSGSHQDAIRKGFA------------- 349 Query: 397 EVSWEQLRDTEWEVPYLPIDPKDVGRDYEAVIRVNSQSGKGGVAYIMKTDHGLQIPRSMQ 456 +Q D WEVPYLPIDP D+GR YEAVIRVNSQSGKGG+AY+++ ++G+ +PR MQ Sbjct: 350 ----QQKPDALWEVPYLPIDPADIGRSYEAVIRVNSQSGKGGIAYLLEQEYGISLPRRMQ 405 Query: 457 VEFSTVVQNVTDAEGGEVNSKAMWDIFATEYLERTAPVEQIALRVENAQTENEDASITAE 516 +EFS VVQ TD G E+ ++ + + +EYL+ P ++ R+ Q EN +++ E Sbjct: 406 IEFSQVVQRETDRLGLEMTAQQIHALLHSEYLQANTPYALVSHRL---QEENGHSAVEVE 462 Query: 517 LIHNGKDVT---VDGRGNGPLAAYANALEKLGIDVEIQEYNQHARTSGDDAEAAAYVLAE 573 + G+ T G+GNG L A L + VEI +YN+HA +G +A+AAAY+ Sbjct: 463 VASQGQGETNLHWRGKGNGALEALVAGLP---VPVEIMDYNEHAIGAGTNAKAAAYIELR 519 Query: 574 VNG-RKVWGVGIAGSITYASLKAVTSAVNRAL 604 VNG R V GVGI +IT AS KA+ SA+NR+L Sbjct: 520 VNGERAVHGVGIDENITTASFKALFSALNRSL 551 Lambda K H 0.316 0.133 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 955 Number of extensions: 34 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 616 Length of database: 559 Length adjustment: 37 Effective length of query: 579 Effective length of database: 522 Effective search space: 302238 Effective search space used: 302238 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 53 (25.0 bits)
Align candidate PfGW456L13_4854 (2-isopropylmalate synthase (EC 2.3.3.13))
to HMM TIGR00970 (leuA: 2-isopropylmalate synthase (EC 2.3.3.13))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00970.hmm # target sequence database: /tmp/gapView.24171.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00970 [M=564] Accession: TIGR00970 Description: leuA_yeast: 2-isopropylmalate synthase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.2e-241 789.1 0.0 1.4e-241 788.8 0.0 1.0 1 lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_4854 2-isopropylmalate synthase (EC 2 Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_4854 2-isopropylmalate synthase (EC 2.3.3.13) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 788.8 0.0 1.4e-241 1.4e-241 1 562 [. 7 550 .. 7 552 .. 0.95 Alignments for each domain: == domain 1 score: 788.8 bits; conditional E-value: 1.4e-241 TIGR00970 1 pskkykpfkaiklsnrkwpdkvitraprwlsvdlrdGnqalidpmsverkkryfk 55 ps+ky+ f +i +++r+wp k it ap w+s dlrdGnq+li+pm++ +k r++k lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_4854 7 PSSKYRAFPTIDIPDRTWPSKTITAAPIWCSSDLRDGNQSLIEPMDAVKKLRFWK 61 99***************************************************** PP TIGR00970 56 llvriGfkeievgfpsasqtdfdfvreiieqglipddvtiqvltqsreelikrtv 110 lv++G keie +fp+asqtdfdfvr +ie + ipdd tiqvltq re+li+rt+ lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_4854 62 TLVQVGVKEIEASFPAASQTDFDFVRTLIEGNHIPDDTTIQVLTQGREDLIERTF 116 ******************************************************* PP TIGR00970 111 ealsGakkaivhlynatsdlfrevvfrasreevlalavegsklvrklvkdaaksk 165 e+l+Gakkaivhlynats+ fr++vf+++++ v a+av+++kl vk aa ++ lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_4854 117 ESLRGAKKAIVHLYNATSPSFRRIVFNQDKDGVKAIAVNAAKL---FVKYAAMQP 168 ****************************************665...78899**** PP TIGR00970 166 etrwsfeyspesfsdtelefavevceavkeviepteerpiifnlpatvevatpnv 220 +t+w+feyspe+fs te+efa+evc+av ev++pt+e+ +i+nlpatve atpn+ lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_4854 169 DTEWTFEYSPETFSATEMEFAKEVCDAVIEVWNPTPEHKMILNLPATVECATPNI 223 ******************************************************* PP TIGR00970 221 yadsieylstniaerekvilslhphndrGtavaaaelGllaGadrieGclfGnGe 275 yad+ie++ ++i r++vi+slh+hndrGt+vaa+elGl+aGadr+eGclfGnGe lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_4854 224 YADQIEWFGRHINRRDSVIISLHTHNDRGTGVAATELGLMAGADRVEGCLFGNGE 278 ******************************************************* PP TIGR00970 276 rtGnvdlvtlalnlytqGvspnldfsdldeilrvvercnkipvherhpygGdlvv 330 rtGnvdlvt+alnlytqGv+p+ldfsd+d +++vve+cn+i+vh+rhpy Gdlv+ lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_4854 279 RTGNVDLVTVALNLYTQGVNPELDFSDIDGVRKVVEECNQIQVHPRHPYVGDLVH 333 ******************************************************* PP TIGR00970 331 tafsGshqdaikkGldaldkkkaaadtlwkvpylpldpkdvgreyeavirvnsqs 385 tafsGshqdai+kG+ ++ d+lw+vpylp+dp d+gr yeavirvnsqs lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_4854 334 TAFSGSHQDAIRKGFAQQK-----PDALWEVPYLPIDPADIGRSYEAVIRVNSQS 383 ***************7654.....4679*************************** PP TIGR00970 386 GkGGvayvlktdlGldlprrlqiefssvvkdiadskGkelsskeisdlfkeeyll 440 GkGG+ay+l +++G++lprr+qiefs+vv+ +d G e+++++i l++ eyl lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_4854 384 GKGGIAYLLEQEYGISLPRRMQIEFSQVVQRETDRLGLEMTAQQIHALLHSEYLQ 438 ******************************************************* PP TIGR00970 441 nveqlerislvdyaveddGteskvitavvkikge...kkdieGsGnGplsalvda 492 ++ ++ +s + e++G + +++ v +g+ + + G GnG l alv lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_4854 439 ANTPYALVSHRL--QEENG--HSAVEVEVASQGQgetNLHWRGKGNGALEALVAG 489 888887776554..46677..344444454444410134589*********9998 PP TIGR00970 493 ladllnvdvavadysehalgsGddakaasyvelsvrrasdaekatvwGvGiaedv 547 l v v+++dy+eha+g+G +akaa+y+el v+ + v GvGi+e++ lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_4854 490 LP----VPVEIMDYNEHAIGAGTNAKAAAYIELRVNGER-----AVHGVGIDENI 535 75....778************************998766.....799******** PP TIGR00970 548 tsaslravlsavnra 562 t+as++a++sa+nr lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_4854 536 TTASFKALFSALNRS 550 *************95 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (564 nodes) Target sequences: 1 (559 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.03 # Mc/sec: 9.38 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory