Alignments for a candidate for lysT in Pseudomonas fluorescens GW456-L13

GapMind for Amino acid biosynthesis

Alignments for a candidate for lysT in Pseudomonas fluorescens GW456-L13

Align Probable methanogen homoaconitase large subunit; HACN; EC 4.2.1.114; Homoaconitate hydratase (uncharacterized)
to candidate PfGW456L13_3592 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33)

Query= curated2:O27668
         (428 letters)



>FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_3592
          Length = 473

 Score =  211 bits (537), Expect = 4e-59
 Identities = 145/440 (32%), Positives = 213/440 (48%), Gaps = 62/440 (14%)

Query: 30  VDLAMTHDGTSPPTIRTFRDIASRGGPARVWDPERIVMVFDH---NVPANTIGAAE---- 82
           +D  + ++ TSP      R+   +      W P   + V DH    VP  T    +    
Sbjct: 30  IDRQVANEYTSPQAFSGMREAGRKA-----WRPGATLAVVDHVNPTVPTRTAAMPDAGGA 84

Query: 83  -----FQRVTREFAREQGIVNIFQNAAGICHQVLPERGFVRPGMVIVGADSHTCTYGAFG 137
                F+   R+F  E  + ++     GI H V PE+GF+ PGMV+   DSHT TYGA G
Sbjct: 85  RQVSYFEENCRDFGIE--LFDVLDKRQGIEHVVAPEQGFILPGMVVAAGDSHTTTYGALG 142

Query: 138 AFATGMGATDMAMVFATGKTWFMVPEAMRIEVTGEPEGHVYAKDVILHIIGEIGVDGATY 197
           AF  G+G +++  + A+    +   + MR+ V G     V +KD+I+ +I +IG  GAT 
Sbjct: 143 AFGFGIGTSEIEHLLASQTLVYKRLKTMRVTVNGVLGTGVTSKDIIMALIEKIGASGATG 202

Query: 198 RSVEFTGDTIESMDVSGRMTICNMAVEMGAKNGIMEPNRQTLDYV----RARTGR----- 248
            ++EF G  I  + V  RMTICNMAVE GA+   M P+ +   Y+    RA  G+     
Sbjct: 203 YAIEFAGPAISDLSVEARMTICNMAVEAGARGAFMAPDDKVFAYLQHKPRAPQGQLWEQA 262

Query: 249 --EFRVYSSDEDSQYLEDHHFDVSDLEPQV---ACPDDVDNV------------------ 285
             ++R   SDE + +  +   DV+ LEP V     PD   +V                  
Sbjct: 263 VAKWRGLHSDEGAVFDREVTLDVAALEPMVTWGTSPDQAASVGGHVPDPAAQPDLILRQG 322

Query: 286 ----------YPVHRVEGTHIDEAFLGSCTNGRYEDLKIAAEVIGDRRVHEDVRFIVSPA 335
                      P   +    I  AF+GSCTN R EDL+  A V+  +RV   VR ++ P 
Sbjct: 323 MQRALNYMGLTPGMPLSDVVISHAFIGSCTNARIEDLRDVARVVRGKRVASHVRAMIVPG 382

Query: 336 SREIYLKALEDGIIETFIRAGAIVCNPGCGPCLGAHMGVLAPGEVSIATTNRNFRGRMGD 395
           S  +  +A ++G+ + F+ AG      GC  CL  +  VLAPG+   ++TNRNF GR G 
Sbjct: 383 STLVRHQAEDEGLAQIFLDAGFEWRQSGCSMCLAMNDDVLAPGDRCASSTNRNFEGRQGA 442

Query: 396 PASSVYLANPAVVAESAIEG 415
            A + +L +PA+VA +AI G
Sbjct: 443 GART-HLMSPAMVAAAAITG 461


Lambda     K      H
   0.320    0.137    0.408 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 533
Number of extensions: 23
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 428
Length of database: 473
Length adjustment: 33
Effective length of query: 395
Effective length of database: 440
Effective search space:   173800
Effective search space used:   173800
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis