Align methionine synthase; EC 2.1.1.13 (characterized)
to candidate PfGW456L13_2847 5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13)
Query= CharProtDB::CH_090726
(1227 letters)
>FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2847
Length = 1236
Score = 1606 bits (4158), Expect = 0.0
Identities = 819/1235 (66%), Positives = 977/1235 (79%), Gaps = 15/1235 (1%)
Query: 2 SSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFADWPCDLKGNNDLLVLSKP 61
S +++ L+ L ERIL+LDGGMGTMIQSY+L E D+RG+RFADWP D+KGNNDLLVL++P
Sbjct: 5 SVRLQALKHALKERILILDGGMGTMIQSYKLEEQDYRGKRFADWPSDVKGNNDLLVLTRP 64
Query: 62 EVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAEINFAAAKLARACADEWTAR 121
+VI I AY +AGADI+ETNTFN+T I+MADY ME L+ E+N A+LAR AD T
Sbjct: 65 DVIGGIEKAYLDAGADILETNTFNATRISMADYGMEKLAYELNVEGARLARKVADAKTLE 124
Query: 122 TPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLVAAYRESTKALVEGGADLILIE 181
P+KPR+VAGVLGPT+RT S+SPDVN+P +RN+TFD LV Y E+TK L+EGGADLILIE
Sbjct: 125 NPDKPRFVAGVLGPTSRTCSLSPDVNNPGYRNVTFDELVENYTEATKGLIEGGADLILIE 184
Query: 182 TVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDASGRTLSGQTTEAFYNSLRHAEAL 241
T+FDTLNAKAA+FAV+ FE LGVELPIMISGTITDASGRTLSGQTTEAF+NS+ HA+ +
Sbjct: 185 TIFDTLNAKAAIFAVQGVFEELGVELPIMISGTITDASGRTLSGQTTEAFWNSVAHAKPI 244
Query: 242 TFGLNCALGPDELRQYVQELSRIAECYVTAHPNAGLPNAFGEYDLDADTMAKQIREWAQA 301
+ GLNCALG ELR Y++ELS A +V+AHPNAGLPN FGEYD AK I E+AQ+
Sbjct: 245 SVGLNCALGARELRPYLEELSNKANTHVSAHPNAGLPNEFGEYDELPSETAKVIEEFAQS 304
Query: 302 GFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLPEIPVACRLSGLEPLNIGEDSLFVNVGE 361
GFLNIVGGCCGTTP HI A+++AV G APR++P+IP ACRLSGLEP I +SLFVNVGE
Sbjct: 305 GFLNIVGGCCGTTPGHIEAIAKAVAGYAPREIPDIPKACRLSGLEPFTIDRNSLFVNVGE 364
Query: 362 RTNVTGSAKFKRLIKEEKYSEALDVARQQVENGAQIIDINMDEGMLDAEAAMVRFLNLIA 421
RTN+TGSAKF RLI+E+ Y+EAL+VA QQVE GAQ+IDINMDEGMLD++ AMV FLNLIA
Sbjct: 365 RTNITGSAKFARLIREDNYTEALEVALQQVEAGAQVIDINMDEGMLDSKKAMVTFLNLIA 424
Query: 422 GEPDIARVPIMIDSSKWDVIEKGLKCIQGKGIVNSISMKEGVDAFIHHAKLLRRYGAAVV 481
GEPDI+RVPIMIDSSKW+VIE GLKCIQGKGIVNSISMKEGV+ FIHHAKL +RYGAAVV
Sbjct: 425 GEPDISRVPIMIDSSKWEVIEAGLKCIQGKGIVNSISMKEGVEQFIHHAKLCKRYGAAVV 484
Query: 482 VMAFDEQGQADTRARKIEICRRAYKILTEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQD 541
VMAFDE GQADT ARK EIC+R+Y IL EV FPPEDIIFDPNIFAVATGIEEHNNYA D
Sbjct: 485 VMAFDEAGQADTEARKKEICKRSYDILVNEVDFPPEDIIFDPNIFAVATGIEEHNNYAVD 544
Query: 542 FIGACEDIKRELPHALISGGVSNVSFSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQ 601
FI AC I+ ELP+AL SGGVSNVSFSFRGN+PVREAIH+VFL YAIR+G+ MGIVNAGQ
Sbjct: 545 FINACAYIRDELPYALTSGGVSNVSFSFRGNNPVREAIHSVFLLYAIRSGLTMGIVNAGQ 604
Query: 602 LAIYDDLPAELRDAVEDVILNRRDDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNK 661
L IYD +P ELRDAVEDVILNR +GT+ LL +A+KY+G A+ EWR+W+VNK
Sbjct: 605 LEIYDQIPVELRDAVEDVILNRTPEGTDALLAIADKYKGD--GSVKEAETEEWRNWDVNK 662
Query: 662 RLEYSLVKGITEFIEQDTEEARQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKS 721
RLE++LVKGIT I +DTEE+RQ RPIEVIEGPLM GMN+VGDLFG GKMFLPQVVKS
Sbjct: 663 RLEHALVKGITTHIIEDTEESRQSFARPIEVIEGPLMAGMNIVGDLFGAGKMFLPQVVKS 722
Query: 722 ARVMKQAVAYLEPFIEASK-EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDL 780
ARVMKQAVA+L PFIE K ++ + GK+++ATVKGDVHDIGKNIVGVVL CN Y+IVDL
Sbjct: 723 ARVMKQAVAHLIPFIELEKGDKPEAKGKILMATVKGDVHDIGKNIVGVVLGCNGYDIVDL 782
Query: 781 GVMVPAEKILRTAKEVNADLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKA 840
GVMVPAEKIL+ A+E D+IGLSGLITPSLDEMV+VA+EM+RQ F +PL+IGGATTSKA
Sbjct: 783 GVMVPAEKILQVAREQKCDIIGLSGLITPSLDEMVHVAREMQRQDFHLPLMIGGATTSKA 842
Query: 841 HTAVKIEQNYSGPTV-YVQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKP 899
HTAVKIE YS V YV +ASR VGV LLS + FV +TR++Y VR + +
Sbjct: 843 HTAVKIEPKYSNDAVIYVTDASRAVGVATQLLSKELKAGFVEKTRQDYIEVRERTSNRSA 902
Query: 900 RTPPVTLEAARDNDFAFDWQAYTPPVAHRLGVQEVE-ASIETLRNYIDWTPFFMTWSLAG 958
RT ++ AA FDW +YTP G + ++ ++ L YIDWTPFF++W LAG
Sbjct: 903 RTERLSYGAAIAKKPQFDWASYTPVKPTFTGARVLDNIDLDVLAEYIDWTPFFISWDLAG 962
Query: 959 KYPRILEDEVVGVEAQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRV-GDDIEIYRDE 1017
K+PRIL+DEVVG A L+ DA +ML KL EK ++ R V G +PAN+V DDIE+Y D+
Sbjct: 963 KFPRILQDEVVGEAATSLYNDAREMLRKLIDEKLISARAVFGFWPANQVHDDDIELYGDD 1022
Query: 1018 TRTHVINVSHHLRQQTEKT-GFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAF 1076
+ + HHLRQQ KT G N+ LADFVAPK S DY+G F T G+ + +A A+
Sbjct: 1023 GKP--MTKLHHLRQQIIKTDGKPNFSLADFVAPKDSEVTDYVGGFITTAGIGAEEVAKAY 1080
Query: 1077 EAQHDDYNKIMVKALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPA 1136
+ DDYN IMVKALADRLAEA AE+LH++VRK YWGYA +E L NE LI+E Y GIRPA
Sbjct: 1081 QDAGDDYNSIMVKALADRLAEACAEWLHQQVRKDYWGYAKDEALDNEALIKEQYSGIRPA 1140
Query: 1137 PGYPACPEHTEKATIWELLEVEK------HTGMKLTESFAMWPGASVSGWYFSHPDSKYY 1190
PGYPACP+HTEKA ++ LL+ E +G+ LTE +AM+P A+VSGWYF+HP ++Y+
Sbjct: 1141 PGYPACPDHTEKAQLFALLDPEAQEMRAGRSGVFLTEHYAMFPAAAVSGWYFAHPQAQYF 1200
Query: 1191 AVAQIQRDQVEDYARRKGMSVTEVERWLAPNLGYD 1225
AV +I +DQV+ Y RKG ++ ERWLAPNLGYD
Sbjct: 1201 AVGKIDKDQVQSYTSRKGQELSVTERWLAPNLGYD 1235
Lambda K H
0.318 0.134 0.391
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 3825
Number of extensions: 169
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 1227
Length of database: 1236
Length adjustment: 47
Effective length of query: 1180
Effective length of database: 1189
Effective search space: 1403020
Effective search space used: 1403020
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 59 (27.3 bits)
Align candidate PfGW456L13_2847 (5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13))
to HMM TIGR02082 (metH: methionine synthase (EC 2.1.1.13))
# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file: ../tmp/path.aa/TIGR02082.hmm
# target sequence database: /tmp/gapView.20438.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
Query: TIGR02082 [M=1182]
Accession: TIGR02082
Description: metH: methionine synthase
Scores for complete sequences (score includes all domains):
--- full sequence --- --- best 1 domain --- -#dom-
E-value score bias E-value score bias exp N Sequence Description
------- ------ ----- ------- ------ ----- ---- -- -------- -----------
0 1756.8 0.0 0 1756.7 0.0 1.0 1 lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2847 5-methyltetrahydrofolate--homocy
Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2847 5-methyltetrahydrofolate--homocysteine methyltransferase (EC
# score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc
--- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ----
1 ! 1756.7 0.0 0 0 1 1182 [] 15 1202 .. 15 1202 .. 0.98
Alignments for each domain:
== domain 1 score: 1756.7 bits; conditional E-value: 0
TIGR02082 1 lnkrilvlDGamGtqlqsanLteadFrge.eadlarelkGnndlLnltkPevi 52
l++ril+lDG+mGt++qs++L+e+d+rg+ +ad+++++kGnndlL+lt+P+vi
lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2847 15 LKERILILDGGMGTMIQSYKLEEQDYRGKrFADWPSDVKGNNDLLVLTRPDVI 67
579************************************************** PP
TIGR02082 53 aaihrayfeaGaDivetntFnsteialadYdledkayelnkkaaklarevade 105
i +ay++aGaDi+etntFn+t i++adY++e++ayeln ++a+lar+vad
lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2847 68 GGIEKAYLDAGADILETNTFNATRISMADYGMEKLAYELNVEGARLARKVADA 120
***************************************************** PP
TIGR02082 106 ft.ltpekkRfvaGslGPtnklatlspdverpefrnvtydelvdaYkeqvkgl 157
t +p+k+RfvaG+lGPt+++ +lspdv++p++rnvt+delv+ Y+e++kgl
lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2847 121 KTlENPDKPRFVAGVLGPTSRTCSLSPDVNNPGYRNVTFDELVENYTEATKGL 173
9989************************************************* PP
TIGR02082 158 ldGGvDllLietvfDtlnakaalfaveevfeekgrelPilisgvivdksGrtL 210
++GG+Dl+Liet+fDtlnakaa+fav+ vfee g+elPi+isg+i+d+sGrtL
lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2847 174 IEGGADLILIETIFDTLNAKAAIFAVQGVFEELGVELPIMISGTITDASGRTL 226
***************************************************** PP
TIGR02082 211 sGqtleaflaslehaeililGLnCalGadelrefvkelsetaealvsviPnaG 263
sGqt+eaf +s+ ha+ +++GLnCalGa elr++++els++a++ vs++PnaG
lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2847 227 SGQTTEAFWNSVAHAKPISVGLNCALGARELRPYLEELSNKANTHVSAHPNAG 279
***************************************************** PP
TIGR02082 264 LPnalgeYdltpeelakalkefaeegllnivGGCCGttPehiraiaeavkdik 316
LPn++geYd++p+e+ak+++efa+ g+lnivGGCCGttP hi aia+av++ +
lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2847 280 LPNEFGEYDELPSETAKVIEEFAQSGFLNIVGGCCGTTPGHIEAIAKAVAGYA 332
***************************************************** PP
TIGR02082 317 prkrqeleeksvlsglealkiaqessfvniGeRtnvaGskkfrklikaedyee 369
pr+ ++++++++lsgle+++i+++s fvn+GeRtn++Gs+kf++li++++y e
lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2847 333 PREIPDIPKACRLSGLEPFTIDRNSLFVNVGERTNITGSAKFARLIREDNYTE 385
***************************************************** PP
TIGR02082 370 alkiakqqveeGaqilDinvDevllDgeadmkkllsllasepdiakvPlmlDs 422
al++a qqve Gaq++Din+De++lD++++m+++l+l+a+epdi++vP+m+Ds
lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2847 386 ALEVALQQVEAGAQVIDINMDEGMLDSKKAMVTFLNLIAGEPDISRVPIMIDS 438
***************************************************** PP
TIGR02082 423 sefevleaGLkviqGkaivnsislkdGeerFlekaklikeyGaavvvmafDee 475
s++ev+eaGLk+iqGk+ivnsis+k+G+e+F+++akl k+yGaavvvmafDe
lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2847 439 SKWEVIEAGLKCIQGKGIVNSISMKEGVEQFIHHAKLCKRYGAAVVVMAFDEA 491
***************************************************** PP
TIGR02082 476 GqartadkkieiakRayklltekvgfppediifDpniltiatGieehdryaid 528
Gqa+t ++k ei+kR y++l+++v+fppediifDpni+++atGieeh++ya+d
lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2847 492 GQADTEARKKEICKRSYDILVNEVDFPPEDIIFDPNIFAVATGIEEHNNYAVD 544
***************************************************** PP
TIGR02082 529 fieaireikeelPdakisgGvsnvsFslrgndavRealhsvFLyeaikaGlDm 581
fi+a+ i+ elP+a +sgGvsnvsFs+rgn++vRea+hsvFL +ai++Gl m
lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2847 545 FINACAYIRDELPYALTSGGVSNVSFSFRGNNPVREAIHSVFLLYAIRSGLTM 597
***************************************************** PP
TIGR02082 582 givnagklavyddidkelrevvedlildrrreatekLlelaelykgtkekssk 634
givnag+l++yd+i+ elr++ved+il+r +e t+ Ll +a++ykg + k
lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2847 598 GIVNAGQLEIYDQIPVELRDAVEDVILNRTPEGTDALLAIADKYKGDGSV--K 648
**********************************************9998..9 PP
TIGR02082 635 eaqeaewrnlpveeRLeralvkGeregieedleearkklkapleiiegpLldG 687
ea+++ewrn++v++RLe+alvkG++ +i ed+ee+r+ +p+e+iegpL++G
lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2847 649 EAETEEWRNWDVNKRLEHALVKGITTHIIEDTEESRQSFARPIEVIEGPLMAG 701
99*************************************************** PP
TIGR02082 688 mkvvGdLFGsGkmfLPqvvksarvmkkavayLePylekekeed.kskGkivla 739
m++vGdLFG+GkmfLPqvvksarvmk+ava+L+P++e ek ++ ++kGki++a
lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2847 702 MNIVGDLFGAGKMFLPQVVKSARVMKQAVAHLIPFIELEKGDKpEAKGKILMA 754
***************************************9877689******* PP
TIGR02082 740 tvkGDvhDiGknivdvvLscngyevvdlGvkvPvekileaakkkkaDviglsG 792
tvkGDvhDiGkniv+vvL+cngy++vdlGv+vP+ekil++a+++k D+iglsG
lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2847 755 TVKGDVHDIGKNIVGVVLGCNGYDIVDLGVMVPAEKILQVAREQKCDIIGLSG 807
***************************************************** PP
TIGR02082 793 LivksldemvevaeemerrgvkiPlllGGaalskahvavkiaekYkg.evvyv 844
Li++sldemv+va+em+r+ +++Pl++GGa++skah+avki++kY+ v+yv
lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2847 808 LITPSLDEMVHVAREMQRQDFHLPLMIGGATTSKAHTAVKIEPKYSNdAVIYV 860
*********************************************872599** PP
TIGR02082 845 kdaseavkvvdkllsekkkaeelekikeeyeeirekfgekkeklialsekaar 897
+das+av v+ +lls++ ka ++ek++++y e+re+ +++ +++ ls aa
lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2847 861 TDASRAVGVATQLLSKELKAGFVEKTRQDYIEVRERTSNRSARTERLSYGAAI 913
***************************************************** PP
TIGR02082 898 kevfaldrsedlevpapkflGtkvleas.ieellkyiDwkalFvqWelrgkyp 949
++ ++d+ +++++p+f G +vl+++ ++ l +yiDw+++F++W+l+gk+p
lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2847 914 AKKPQFDWA-SYTPVKPTFTGARVLDNIdLDVLAEYIDWTPFFISWDLAGKFP 965
*********.9****************************************** PP
TIGR02082 950 kilkdeleglearklfkdakelldklsaekllrargvvGlfPaqsv.gddiei 1001
+il+de++g+ a+ l++da+e+l kl+ ekl+ ar+v+G++Pa++v +ddie+
lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2847 966 RILQDEVVGEAATSLYNDAREMLRKLIDEKLISARAVFGFWPANQVhDDDIEL 1018
*******************************************9761578*** PP
TIGR02082 1002 ytdetvsqetkpiatvrekleqlrqqsdr.ylclaDfiaskesGikDylgall 1053
y d++ p++ +++ ++q+ + ++ + +laDf+a+k+s +Dy+g ++
lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2847 1019 YGDDGK-----PMTKLHHLRQQIIKTDGKpNFSLADFVAPKDSEVTDYVGGFI 1066
**9887.....5555555556666666656*********************** PP
TIGR02082 1054 vtaglgaeelakkleakeddydsilvkaladrlaealaellhervRkelwgya 1106
+tag+gaee ak++++ ddy+si+vkaladrlaea ae+lh++vRk++wgya
lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2847 1067 TTAGIGAEEVAKAYQDAGDDYNSIMVKALADRLAEACAEWLHQQVRKDYWGYA 1119
***************************************************** PP
TIGR02082 1107 eeenldkedllkerYrGirpafGYpacPdhtekatlleLleae.......riG 1152
++e+ld+e l+ke+Y Girpa+GYpacPdhteka+l++Ll++e r G
lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2847 1120 KDEALDNEALIKEQYSGIRPAPGYPACPDHTEKAQLFALLDPEaqemragRSG 1172
****************************************9875555444569 PP
TIGR02082 1153 lklteslalaPeasvsglyfahpeakYfav 1182
+ lte +a+ P+a+vsg+yfahp+a+Yfav
lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2847 1173 VFLTEHYAMFPAAAVSGWYFAHPQAQYFAV 1202
****************************98 PP
Internal pipeline statistics summary:
-------------------------------------
Query model(s): 1 (1182 nodes)
Target sequences: 1 (1236 residues searched)
Passed MSV filter: 1 (1); expected 0.0 (0.02)
Passed bias filter: 1 (1); expected 0.0 (0.02)
Passed Vit filter: 1 (1); expected 0.0 (0.001)
Passed Fwd filter: 1 (1); expected 0.0 (1e-05)
Initial search space (Z): 1 [actual number of targets]
Domain search space (domZ): 1 [number of targets reported over threshold]
# CPU time: 0.08u 0.03s 00:00:00.11 Elapsed: 00:00:00.10
# Mc/sec: 13.38
//
[ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory