Alignments for a candidate for argC in Pseudomonas fluorescens GW456-L13

GapMind for Amino acid biosynthesis

Alignments for a candidate for argC in Pseudomonas fluorescens GW456-L13

Align N-acetyl-gamma-glutamyl-phosphate reductase; Short=AGPR; EC 1.2.1.38 (characterized, see rationale)
to candidate PfGW456L13_1233 N-acetyl-gamma-glutamyl-phosphate reductase (EC 1.2.1.38)

Query= uniprot:E4PLW0
         (388 letters)



>FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1233
          Length = 344

 Score =  472 bits (1214), Expect = e-138
 Identities = 234/346 (67%), Positives = 276/346 (79%), Gaps = 3/346 (0%)

Query: 44  VIKVGIVGGTGYTGVELLRILAVHPEVSVSCITSRSEAGMPVAEMYPNLRGHYD-LAFSE 102
           ++KVGIVGGTGYTGVELLR+LA HP+  V  ITSRSEAG+ VA+MYPNLRGHYD LAFS 
Sbjct: 1   MVKVGIVGGTGYTGVELLRLLAQHPQAEVVVITSRSEAGLAVADMYPNLRGHYDGLAFSV 60

Query: 103 PDVNVLGACDLVFFATPHGVAMRMVPELMSAGVRVVDLSADFRLKDLDVWANWYGMAHES 162
           PD+  LGACD+VFFATPHGVA  +  EL++AG +V+DLSADFRL+D D WA WYG  H +
Sbjct: 61  PDIKTLGACDVVFFATPHGVAHALAGELLAAGTKVIDLSADFRLQDADEWAKWYGQPHGA 120

Query: 163 PEWAEKAVYGLPEVVRDEIRNAQLVANPGCYPTAVQLGFLPLLEQGLVDPKRLIADAKSG 222
           PE  ++AVYGLPEV R++I+ A+L+A PGCYPTA QLGFLPLLE GL D   LIAD KSG
Sbjct: 121 PELLDEAVYGLPEVNREQIKKARLIAVPGCYPTATQLGFLPLLEAGLADAAHLIADCKSG 180

Query: 223 ASGAGRQGKIGMLHGEIGESFKAYGASGHRHLPEIRQGLCGAAGGDVGVTFVPHLIPMIR 282
            SGAGR   +G L+ E  ES KAY   GHRHLPEIRQGL  AAG DVG+TFVPHL PMIR
Sbjct: 181 VSGAGRGASVGSLYSETSESMKAYAVKGHRHLPEIRQGLRRAAGKDVGLTFVPHLTPMIR 240

Query: 283 GIEATLYAELKNPADFDRLQALFEQRFDDEPFVDVMPFGSHPETRSVRGANQCRMALHRQ 342
           GI +TLYA + + +    LQALFE+R+ +EPFVDVMP GSHPETRSVRGAN CR+A+HR 
Sbjct: 241 GIHSTLYATVVDRS--VDLQALFEKRYANEPFVDVMPAGSHPETRSVRGANVCRIAVHRP 298

Query: 343 EQSNIVIVSSVIDNLVKGAAGQAVQNMNIMFGLKETMGLEAPALLP 388
           +  ++V+V SVIDNLVKGA+GQAVQNMNI+FGL E +GL    +LP
Sbjct: 299 QDGDLVVVLSVIDNLVKGASGQAVQNMNILFGLDERLGLSHAGMLP 344


Lambda     K      H
   0.321    0.137    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 463
Number of extensions: 21
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 388
Length of database: 344
Length adjustment: 30
Effective length of query: 358
Effective length of database: 314
Effective search space:   112412
Effective search space used:   112412
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 49 (23.5 bits)

Align candidate PfGW456L13_1233 (N-acetyl-gamma-glutamyl-phosphate reductase (EC 1.2.1.38))
to HMM TIGR01850 (argC: N-acetyl-gamma-glutamyl-phosphate reductase (EC 1.2.1.38))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01850.hmm
# target sequence database:        /tmp/gapView.21867.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01850  [M=345]
Accession:   TIGR01850
Description: argC: N-acetyl-gamma-glutamyl-phosphate reductase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                               Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                               -----------
   2.5e-136  440.4   0.0   2.8e-136  440.2   0.0    1.0  1  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1233  N-acetyl-gamma-glutamyl-phosphat


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1233  N-acetyl-gamma-glutamyl-phosphate reductase (EC 1.2.1.38)
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  440.2   0.0  2.8e-136  2.8e-136       1     345 []       2     344 .]       2     344 .] 0.98

  Alignments for each domain:
  == domain 1  score: 440.2 bits;  conditional E-value: 2.8e-136
                                               TIGR01850   1 ikvaivGasGYtGaeLlrllakHpevevtklvssreagkklsevhphlkglvd.l 54 
                                                             +kv+ivG++GYtG+eLlrlla+Hp++ev+ ++s++eag  +++++p+l+g++d l
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1233   2 VKVGIVGGTGYTGVELLRLLAQHPQAEVVVITSRSEAGLAVADMYPNLRGHYDgL 56 
                                                             69***************************8888888*****************88 PP

                                               TIGR01850  55 kleeleeeeileeadvvflAlphgvsaelvpellekgvkvidlSadfRlkdaevY 109
                                                              ++  +++ +l ++dvvf+A+phgv+++l+ ell++g+kvidlSadfRl+da+++
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1233  57 AFSVPDIK-TLGACDVVFFATPHGVAHALAGELLAAGTKVIDLSADFRLQDADEW 110
                                                             88887777.567******************************************* PP

                                               TIGR01850 110 ekwYgkkhekeelleeavYGlpElnreeikkaklianPGCyaTaalLalaPllke 164
                                                             +kwYg++h ++ell+eavYGlpE+nre+ikka+lia+PGCy+Ta++L+  Pll++
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1233 111 AKWYGQPHGAPELLDEAVYGLPEVNREQIKKARLIAVPGCYPTATQLGFLPLLEA 165
                                                             ******************************************************* PP

                                               TIGR01850 165 kliepksiivdaksGvSgAGrkasekslfaevnenlkpYkvtkHrHtpEieqels 219
                                                             +l +  ++i d+ksGvSgAGr as  sl++e++e++k+Y+v++HrH pEi+q l+
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1233 166 GLADAAHLIADCKSGVSGAGRGASVGSLYSETSESMKAYAVKGHRHLPEIRQGLR 220
                                                             ******************************************************* PP

                                               TIGR01850 220 klaekkvkvsftphlvpmtrGilatiyaklkkelteeelrklyeevYedepfvrv 274
                                                             ++a+k+v ++f+phl+pm+rGi++t+ya++ +  ++ +l++l+e++Y++epfv+v
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1233 221 RAAGKDVGLTFVPHLTPMIRGIHSTLYATVVD--RSVDLQALFEKRYANEPFVDV 273
                                                             *******************************9..789****************** PP

                                               TIGR01850 275 lkegelPstkavlgsnfvdigvavdeetkrvvvvsaiDNLvKGaagqAvqnlNlm 329
                                                             +++g+ P+t++v+g n+++i+v+  ++ + vvv+s+iDNLvKGa+gqAvqn+N+ 
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1233 274 MPAGSHPETRSVRGANVCRIAVHRPQDGDLVVVLSVIDNLVKGASGQAVQNMNIL 328
                                                             ******************************************************* PP

                                               TIGR01850 330 lgfdetegLeklpllp 345
                                                             +g+de+ gL++ ++lp
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_1233 329 FGLDERLGLSHAGMLP 344
                                                             ***********99987 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (345 nodes)
Target sequences:                          1  (344 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01
# Mc/sec: 6.60
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis