Alignments for a candidate for cmutase in Pseudomonas fluorescens GW456-L13

GapMind for Amino acid biosynthesis

Alignments for a candidate for cmutase in Pseudomonas fluorescens GW456-L13

Align chorismate mutase (EC 5.4.99.5) (characterized)
to candidate PfGW456L13_2709 Periplasmic chorismate mutase I precursor (EC 5.4.99.5)

Query= BRENDA::B2JYH9
         (182 letters)



>FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2709
          Length = 190

 Score =  115 bits (288), Expect = 5e-31
 Identities = 66/161 (40%), Positives = 94/161 (58%), Gaps = 8/161 (4%)

Query: 22  DAFVPLVRSMADRLNTADQVALSKWDTGQPVYDGQREAQVIANAATMASEYGLTAEDAIN 81
           DA  PL+ +M +RLN ++ VAL+KWD+G+PV D  REAQVIANA   A+E  L  +D   
Sbjct: 33  DALQPLLATMNERLNISELVALTKWDSGKPVQDNTREAQVIANARKQAAERQLDPDDVAV 92

Query: 82  IFSDQVEANKEVQYALLNNWRRQGDAPATPRQSLAGVIRPILDKLQASIMQNLQSVAPLR 141
           + + Q+EA+K VQY  L  W+    AP TPR  L   IRP LD LQ  ++    +  P R
Sbjct: 93  LIAAQIEASKLVQYGRLAQWQAAHKAPDTPRPDLGNDIRPKLDNLQNLLLTQYAAFLPYR 152

Query: 142 SIADCHALVASAVGQVAEQASL--DVLHRAALDRAVARICV 180
           +  +C   +A+      E+++L  D LH  A+ RA   +C+
Sbjct: 153 NDPNCPQWLAT------ERSALIKDYLHGQAMIRATGELCI 187


Lambda     K      H
   0.318    0.129    0.369 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 77
Number of extensions: 4
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 182
Length of database: 190
Length adjustment: 20
Effective length of query: 162
Effective length of database: 170
Effective search space:    27540
Effective search space used:    27540
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 44 (21.6 bits)

Align candidate PfGW456L13_2709 (Periplasmic chorismate mutase I precursor (EC 5.4.99.5))
to HMM TIGR01806 (putative chorismate mutase (EC 5.4.99.5))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01806.hmm
# target sequence database:        /tmp/gapView.5177.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01806  [M=114]
Accession:   TIGR01806
Description: CM_mono2: putative chorismate mutase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                               Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                               -----------
    1.7e-35  107.8   1.2    2.2e-35  107.5   1.2    1.1  1  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2709  Periplasmic chorismate mutase I 


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2709  Periplasmic chorismate mutase I precursor (EC 5.4.99.5)
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  107.5   1.2   2.2e-35   2.2e-35       4     114 .]      33     145 ..      30     145 .. 0.97

  Alignments for each domain:
  == domain 1  score: 107.5 bits;  conditional E-value: 2.2e-35
                                               TIGR01806   4 aaldqlvdlaneRleladaValyKaesnlpieDsereeqvLdslraqaksaglde 58 
                                                              al+ l+  +neRl++++ Val K++s++p++D+ re+qv++++r+qa +  ld+
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2709  33 DALQPLLATMNERLNISELVALTKWDSGKPVQDNTREAQVIANARKQAAERQLDP 87 
                                                             5789999************************************************ PP

                                               TIGR01806  59 dsverlfqaqinAnkaiqyrllsdWk.skaeppvevrdLe.dlRakidqlntelL 111
                                                             d+v+ l++aqi+A+k++qy +l++W+  +++p ++++dL  d+R+k+d+l++ lL
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2709  88 DDVAVLIAAQIEASKLVQYGRLAQWQaAHKAPDTPRPDLGnDIRPKLDNLQNLLL 142
                                                             **************************999999*******99************** PP

                                               TIGR01806 112 eal 114
                                                             +++
  lcl|FitnessBrowser__pseudo13_GW456_L13:PfGW456L13_2709 143 TQY 145
                                                             986 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (114 nodes)
Target sequences:                          1  (190 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00
# Mc/sec: 4.97
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis