GapMind for Amino acid biosynthesis


Finding step ilvE for L-leucine biosynthesis in Pseudomonas fluorescens FW300-N1B4

4 candidates for ilvE: leucine transaminase

Score Gene Description Similar to Id. Cov. Bits Other hit Other id. Other bits
hi Pf1N1B4_4476 Branched-chain amino acid aminotransferase (EC Branched-chain amino acid aminotransferase (EC (characterized) 96% 100% 671
hi Pf1N1B4_4476 Branched-chain amino acid aminotransferase (EC ilvE: branched-chain amino acid aminotransferase (EC (TIGR01123) 100% 460.5
med Pf1N1B4_27 Biosynthetic Aromatic amino acid aminotransferase alpha (EC @ Aromatic-amino-acid aminotransferase (EC aromatic-amino-acid transaminase TyrB; EC (characterized) 52% 100% 409.1 Aromatic-amino-acid aminotransferase (EC 52% 405.2
med Pf1N1B4_3564 Aspartate aminotransferase (EC aromatic-amino-acid transaminase TyrB; EC (characterized) 43% 99% 313.9 tyrosine aminotransferase (EC 94% 751.9
lo Pf1N1B4_3771 Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC branched-chain-amino-acid transaminase (EC (characterized) 36% 94% 258.1 Aromatic-amino-acid aminotransferase 1; ARAT-I; AROAT; EC 37% 258.8

Confidence: high confidence medium confidence low confidence
? – known gap: despite the lack of a good candidate for this step, this organism (or a related organism) performs the pathway

Also see fitness data for the candidates

Definition of step ilvE

Or cluster all characterized ilvE proteins

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.



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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer. Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory