Alignments for a candidate for leuB in Pseudomonas fluorescens FW300-N1B4

GapMind for Amino acid biosynthesis

Alignments for a candidate for leuB in Pseudomonas fluorescens FW300-N1B4

Align D-malate dehydrogenase (decarboxylating) (EC 1.1.1.83) (characterized)
to candidate Pf1N1B4_4412 Tartrate dehydrogenase (EC 1.1.1.93) @ Tartrate decarboxylase (EC 4.1.1.73) @ D-malic enzyme (EC 1.1.1.83)

Query= BRENDA::P76251
         (361 letters)



>FitnessBrowser__pseudo1_N1B4:Pf1N1B4_4412
          Length = 351

 Score =  353 bits (907), Expect = e-102
 Identities = 189/361 (52%), Positives = 245/361 (67%), Gaps = 11/361 (3%)

Query: 2   MKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQMEWASCEYYSHHGKMMPDD 61
           M   +IAA+PGDGIG EV+  G+ VLQA +++ GF L+F+  +W S + Y  +G  +P+ 
Sbjct: 1   MSAYKIAAVPGDGIGVEVIAAGVEVLQALSKKSGFDLTFKHYDWNS-DNYLKNGYYIPEG 59

Query: 62  WHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQYVNLRPVRLFPGVPCPLAG 121
             E+L  FDAI+FGAVG  + VPDHISLWG  L   + FDQY N+RP R+ PGV  PL  
Sbjct: 60  GLEELKTFDAIFFGAVGALN-VPDHISLWGLRLPICQGFDQYANVRPARVLPGVKSPLLN 118

Query: 122 KQPGD-IDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQESVFTRRGVDRILRYAFELAQS 180
              GD ID+ VVREN+EGEYS  GGRV+ G   EV  + SVFTR GV+RI R+AFELA+S
Sbjct: 119 ---GDQIDWVVVRENSEGEYSGNGGRVHRGLPEEVATEVSVFTRAGVERIHRFAFELARS 175

Query: 181 RPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRWDKQHIDILCARFVMQPERFDVV 240
           RPRK LT  TKSN     M  WDE    +A+++P+++ DK+ +D +  R V++P   DV+
Sbjct: 176 RPRKHLTMVTKSNAQRHGMVLWDEIFYEVAKDFPDVKIDKELVDAVTTRMVLKPSTLDVI 235

Query: 241 VASNLFGDILSDLGPACTGTIGIAPSANLNPERTFPSLFEPVHGSAPDIYGKNIANPIAT 300
           VA+NL  DILSDL  A +G++GIAP+ANLNP R FPS+FEP+HGSA DI GK +ANPIAT
Sbjct: 236 VATNLHADILSDLAAALSGSLGIAPTANLNPSRKFPSMFEPIHGSAFDITGKGVANPIAT 295

Query: 301 IWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIAHGPKTPDMKGNATTPQVADAICKIIL 360
            W  AMML+ LG        A   +++AIE V   G  TPD+ GNATT Q+ DA+  +I 
Sbjct: 296 FWTAAMMLEHLGE-----TTAAKQLMSAIEAVTESGLHTPDLGGNATTRQITDAVIALIN 350

Query: 361 R 361
           R
Sbjct: 351 R 351


Lambda     K      H
   0.321    0.138    0.432 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 400
Number of extensions: 15
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 361
Length of database: 351
Length adjustment: 29
Effective length of query: 332
Effective length of database: 322
Effective search space:   106904
Effective search space used:   106904
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 49 (23.5 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis