Alignments for a candidate for dapA in Pseudomonas fluorescens FW300-N1B4

GapMind for Amino acid biosynthesis

Alignments for a candidate for dapA in Pseudomonas fluorescens FW300-N1B4

Align 4-hydroxy-tetrahydrodipicolinate synthase; HTPA synthase; EC 4.3.3.7 (characterized)
to candidate Pf1N1B4_5813 4-hydroxy-tetrahydrodipicolinate synthase (EC 4.3.3.7)

Query= SwissProt::Q9X1K9
         (294 letters)



>FitnessBrowser__pseudo1_N1B4:Pf1N1B4_5813
          Length = 291

 Score =  161 bits (408), Expect = 1e-44
 Identities = 100/289 (34%), Positives = 157/289 (54%), Gaps = 11/289 (3%)

Query: 2   FRGVGTAIVTPFKNGELDLESYERLVRYQLENGVNALIVLGTTGESPTVNEDEREKLVSR 61
           F+G+   IVTPF +G +D     RLV + LE GV+ ++V GTTGE+  + + E+  ++  
Sbjct: 4   FQGIWVPIVTPFHDGAIDFAGLRRLVSHLLEKGVDGIVVCGTTGEAAALGKHEQLAVLDA 63

Query: 62  TLEIVDGKIPVIVGAGTNSTEKTLKLVKQAEKLGANGVLVVTPYYNKPTQEGLYQHYKYI 121
            LE+V  +  V++G   N+  + L+   +  K    G+LV  PYY +P+Q GL   +K +
Sbjct: 64  VLELVPPE-RVVMGLAGNNLAELLQFQSEILKRPLAGLLVPPPYYIRPSQAGLEAFFKTV 122

Query: 122 SERTDLGIVVYNVPGRTGVNVLPETAARIAADLKNVVGIKEA--NPDIDQIDRTVSLTKQ 179
           ++ + + +++Y++P RTG+     T  RI A  + +V IK+   NP       T++L   
Sbjct: 123 ADASSVPVILYDIPYRTGIAFEQATLLRIVAH-ERIVAIKDCGGNPAT-----TLALLSS 176

Query: 180 ARSDFMVWSGNDDRTFYLLCAGGDGVISVVSNVAPKQMVELCAEYFSGNLEKSREVHRKL 239
            + D  V  G D + F  LC G  G I+  ++V P+  V L  +     L   R    +L
Sbjct: 177 GKVD--VLCGEDHQIFGALCLGATGAIAASAHVHPELFVALYQQIRDNQLAVGRATFFQL 234

Query: 240 RPLMKALFVETNPIPVKAALNLMGFIENELRLPLVPASEKTVELLRNVL 288
            PL+ +LF+E NP PVK AL L G I +ELR P+  +SE T   L+ VL
Sbjct: 235 LPLIHSLFIEPNPAPVKTALALEGLIRDELRSPMQRSSETTAVRLKEVL 283


Lambda     K      H
   0.316    0.135    0.378 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 194
Number of extensions: 5
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 294
Length of database: 291
Length adjustment: 26
Effective length of query: 268
Effective length of database: 265
Effective search space:    71020
Effective search space used:    71020
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.5 bits)
S2: 48 (23.1 bits)

Align candidate Pf1N1B4_5813 (4-hydroxy-tetrahydrodipicolinate synthase (EC 4.3.3.7))
to HMM TIGR00674 (dapA: 4-hydroxy-tetrahydrodipicolinate synthase (EC 4.3.3.7))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00674.hmm
# target sequence database:        /tmp/gapView.14592.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00674  [M=286]
Accession:   TIGR00674
Description: dapA: 4-hydroxy-tetrahydrodipicolinate synthase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                      Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                      -----------
    7.9e-76  240.6   0.0      9e-76  240.4   0.0    1.0  1  lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_5813  4-hydroxy-tetrahydrodipicolinate


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_5813  4-hydroxy-tetrahydrodipicolinate synthase (EC 4.3.3.7)
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  240.4   0.0     9e-76     9e-76       4     284 ..       9     284 ..       6     286 .. 0.96

  Alignments for each domain:
  == domain 1  score: 240.4 bits;  conditional E-value: 9e-76
                                      TIGR00674   4 tAliTPfkedgsvdfaalekliesqiekgvdaivvvGtTGEsatLsleEkkkvievavelvknr 67 
                                                    + ++TPf++   +dfa l +l+++++ekgvd+ivv+GtTGE+a+L + E+ +v+  ++elv   
  lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_5813   9 VPIVTPFHDGA-IDFAGLRRLVSHLLEKGVDGIVVCGTTGEAAALGKHEQLAVLDAVLELVPP- 70 
                                                    679****8877.*************************************************97. PP

                                      TIGR00674  68 vpviaGtgsnateeaieltkeaeklgvdgvlvvtPyYnkPtqeGlykhfkaiaeevelPiilYn 131
                                                     +v+ G ++n+++e +++ +e+ k + +g+lv  PyY +P+q Gl ++fk++a+++++P+ilY+
  lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_5813  71 ERVVMGLAGNNLAELLQFQSEILKRPLAGLLVPPPYYIRPSQAGLEAFFKTVADASSVPVILYD 134
                                                    89************************************************************** PP

                                      TIGR00674 132 vPsRtgvslepetvkrLaeeveivaiKeasgdlervseikaeakedfkvlsGdDaltleilalG 195
                                                    +P Rtg+ +e+ t++r++++++ivaiK+++g+ +    + +  + ++ vl+G+D+     l+lG
  lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_5813 135 IPYRTGIAFEQATLLRIVAHERIVAIKDCGGNPATTLALLS--SGKVDVLCGEDHQIFGALCLG 196
                                                    *******************************8776666665..668****************** PP

                                      TIGR00674 196 akGviSVasnvapkelkemvkaalegdteeareihqkllklfkalfietNPipvKtalallgli 259
                                                    a+G i   ++v p+++  +++++ +++ +  r    +ll+l++ lfie+NP pvKtalal+gli
  lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_5813 197 ATGAIAASAHVHPELFVALYQQIRDNQLAVGRATFFQLLPLIHSLFIEPNPAPVKTALALEGLI 260
                                                    **************************************************************** PP

                                      TIGR00674 260 ekdelRlPLtelseekkeklkevlk 284
                                                       elR P+   se +  +lkevl+
  lcl|FitnessBrowser__pseudo1_N1B4:Pf1N1B4_5813 261 RD-ELRSPMQRSSETTAVRLKEVLA 284
                                                    *9.******************9996 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (286 nodes)
Target sequences:                          1  (291 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00
# Mc/sec: 11.14
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis