GapMind for Amino acid biosynthesis

 

Alignments for a candidate for ilvE in Pseudomonas fluorescens FW300-N1B4

Align tyrosine aminotransferase (EC 2.6.1.57) (characterized)
to candidate Pf1N1B4_3564 Aspartate aminotransferase (EC 2.6.1.1)

Query= reanno::pseudo13_GW456_L13:PfGW456L13_4396
         (397 letters)



>FitnessBrowser__pseudo1_N1B4:Pf1N1B4_3564
          Length = 397

 Score =  751 bits (1940), Expect = 0.0
 Identities = 371/397 (93%), Positives = 382/397 (96%)

Query: 1   MHFDAIGRVPGDPILGLMEAYAQDPNPRKFDLGVGVYKDAQGLTPILESVKLAEQRLVDH 60
           MHFDAIGRVPGDPILGLMEAYAQDPNPRKFDLGVGVYKDAQGLTPI E+VKLAEQRLVD 
Sbjct: 1   MHFDAIGRVPGDPILGLMEAYAQDPNPRKFDLGVGVYKDAQGLTPIPEAVKLAEQRLVDR 60

Query: 61  QSTKTYIGGHGEPAFGKAINELVLGADSKLISAQRAGATQTPGGTGALRLSADFIAQCLP 120
           Q TKTYIGGHG+ AFGK INELVLGADS LI+ QRAGATQTPGGTGALRLSADFIAQCLP
Sbjct: 61  QPTKTYIGGHGDAAFGKVINELVLGADSALIAEQRAGATQTPGGTGALRLSADFIAQCLP 120

Query: 121 GRGVWLSNPTWPIHETIFAAAKVKVSHYPYVGSDNRLDVDGMLAALNEAPKGDVVLLHAC 180
           GRGVWLSNPTWPIHETIFAAA VKVSHYPYVGSDNRLDV+ MLA LN+APKGDVVLLHAC
Sbjct: 121 GRGVWLSNPTWPIHETIFAAAGVKVSHYPYVGSDNRLDVEAMLAVLNQAPKGDVVLLHAC 180

Query: 181 CHNPTGFDLSQDDWQRVLEVVRKRELLPLIDFAYQGFGDGLEQDAWSTRLFAAELPELLI 240
           CHNPTGFDLS DDW+RVL+VVR R+LLPLIDFAYQGFGDGLEQDAWSTRLFAAELPE+LI
Sbjct: 181 CHNPTGFDLSHDDWRRVLDVVRSRDLLPLIDFAYQGFGDGLEQDAWSTRLFAAELPEVLI 240

Query: 241 TSSCSKNFGLYRDRTGALIVCAKTADKLVDIRSQLANIARNLWSTPPDHGAAVVATILGD 300
           TSSCSKNFGLYRDRTGALIVCAKTADKLVDIRSQLANIARNLWSTPPDHGAAVVATILGD
Sbjct: 241 TSSCSKNFGLYRDRTGALIVCAKTADKLVDIRSQLANIARNLWSTPPDHGAAVVATILGD 300

Query: 301 PELKRLWADEVEAMRLRIAQLRSGLVEALEPHGLRERFAHIGVQRGMFSYTGLSPEQVKN 360
           PELK  WADEVEAMRLRIAQLRSGLVEALEPHGLRERFAHIGVQRGMFSYTGLSPEQVK 
Sbjct: 301 PELKSRWADEVEAMRLRIAQLRSGLVEALEPHGLRERFAHIGVQRGMFSYTGLSPEQVKQ 360

Query: 361 LRDHHSVYMVSSGRANVAGIDATRLDLLAEAIASVCK 397
           LR+HHSVYMVSSGRANVAGIDATRLDLLAEAIA+VCK
Sbjct: 361 LREHHSVYMVSSGRANVAGIDATRLDLLAEAIANVCK 397


Lambda     K      H
   0.320    0.137    0.413 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 650
Number of extensions: 13
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 397
Length of database: 397
Length adjustment: 31
Effective length of query: 366
Effective length of database: 366
Effective search space:   133956
Effective search space used:   133956
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

Links

Downloads

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory