Aligments for a candidate for ilvE in Pseudomonas fluorescens FW300-N1B4

GapMind for Amino acid biosynthesis

Aligments for a candidate for ilvE in Pseudomonas fluorescens FW300-N1B4

Align aspartate transaminase (EC 2.6.1.1); glutamate-prephenate aminotransferase (EC 2.6.1.79) (characterized)
to candidate Pf1N1B4_5070 Aspartate aminotransferase (EC 2.6.1.1)

Query= BRENDA::Q02635
         (400 letters)



>FitnessBrowser__pseudo1_N1B4:Pf1N1B4_5070
          Length = 404

 Score =  461 bits (1185), Expect = e-134
 Identities = 222/397 (55%), Positives = 286/397 (72%)

Query: 4   LADALSRVKPSATIAVSQKARELKAKGRDVIGLGAGEPDFDTPDNIKKAAIDAIDRGETK 63
           L   L+  +PSAT  +  +  E +A+G  +I L AGEPDFDTP ++++AAI AI+ G T+
Sbjct: 8   LNQRLASAQPSATYRIMDRVAERRAQGAKIISLCAGEPDFDTPKHVREAAIHAIEHGHTR 67

Query: 64  YTPVSGIPELREAIAKKFKRENNLDYTAAQTIVGTGGKQILFNAFMATLNPGDEVVIPAP 123
           YT V+G+  LREA+A KF+REN LD +   T+V  GGKQ+++NA  ATLN GD+V++PAP
Sbjct: 68  YTQVAGVRSLREAVAAKFRRENGLDVSWQDTLVCNGGKQVIYNALAATLNEGDQVIVPAP 127

Query: 124 YWVSYPEMVALCGGTPVFVPTRQENNFKLKAEDLDRAITPKTKWFVFNSPSNPSGAAYSH 183
           YWVSYPEMV LCGG    V    +  FKL    L  AITP+T+W + NSPSNP+GA YS 
Sbjct: 128 YWVSYPEMVQLCGGEARIVTCDADTGFKLTPAALAAAITPQTRWLILNSPSNPTGAVYSE 187

Query: 184 EELKALTDVLMKHPHVWVLTDDMYEHLTYGDFRFATPVEVEPGLYERTLTMNGVSKAYAM 243
            EL+AL  VL+ HPHV +L DD+YEHL + D  F T  +VEP L  RTLTMNGVSKAYAM
Sbjct: 188 TELRALAAVLLDHPHVLILADDIYEHLIFDDQAFYTLAQVEPRLAPRTLTMNGVSKAYAM 247

Query: 244 TGWRIGYAAGPLHLIKAMDMIQGQQTSGAASIAQWAAVEALNGPQDFIGRNKEIFQGRRD 303
           TGWRIG+A GP  L++AM+ +QGQQTSGA+S++Q AA+ AL GP+DFI  ++  FQ RRD
Sbjct: 248 TGWRIGFATGPRWLLEAMEKLQGQQTSGASSVSQQAALAALEGPKDFIRESRAAFQARRD 307

Query: 304 LVVSMLNQAKGISCPTPEGAFYVYPSCAGLIGKTAPSGKVIETDEDFVSELLETEGVAVV 363
           L+V++LN   G+ C +P GAFY + SCA LIG+T+P+G+V+ TDED    LL+   VAVV
Sbjct: 308 LMVTLLNDTPGLECVSPAGAFYAFASCARLIGRTSPAGRVLHTDEDVAHALLDEADVAVV 367

Query: 364 HGSAFGLGPNFRISYATSEALLEEACRRIQRFCAACR 400
           HGSAFGLGP  RI+YA  +A L +AC  I+ FC A R
Sbjct: 368 HGSAFGLGPYIRIAYALDDASLRQACEAIRAFCTALR 404


Lambda     K      H
   0.318    0.134    0.402 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 473
Number of extensions: 19
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 400
Length of database: 404
Length adjustment: 31
Effective length of query: 369
Effective length of database: 373
Effective search space:   137637
Effective search space used:   137637
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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Steps (tab-delimited)
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Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis