Alignments for a candidate for gatA in Pseudomonas fluorescens FW300-N2E3

GapMind for Amino acid biosynthesis

Alignments for a candidate for gatA in Pseudomonas fluorescens FW300-N2E3

Align Glutamyl-tRNA(Gln) amidotransferase subunit A; Glu-ADT subunit A; EC 6.3.5.7 (uncharacterized)
to candidate AO353_25440 AO353_25440 amidase

Query= curated2:Q72L58
         (471 letters)



>FitnessBrowser__pseudo3_N2E3:AO353_25440
          Length = 483

 Score =  191 bits (486), Expect = 4e-53
 Identities = 160/481 (33%), Positives = 228/481 (47%), Gaps = 48/481 (9%)

Query: 3   AHEIRARVARGEVSPLEVAQAYLKRVQELDPGLGAFLSLN--ERLLEEAEAVDPGLP--- 57
           AHE+   +   +VS  EV QAYL  ++  +P + A +SL   E LL +A+  D  L    
Sbjct: 14  AHELADCIRLRQVSCREVMQAYLSHIERFNPWVNALISLQAPEDLLAQADQRDAELASGL 73

Query: 58  ----LAGLVVAVKDNIATRGLRTTAGSRLLENFVPPYEATAVARLKALGALVLGKTNLDE 113
               + GL  A+KD   TRG+RTT GS L E+F+P  +     R+KA GA+++GKTN  E
Sbjct: 74  YRGWMHGLPHAIKDLSLTRGIRTTFGSPLFEDFLPDRDGIMCERIKAAGAIIIGKTNTPE 133

Query: 114 FGMGSSTEHSAFFPTKNPFDPDRVPGGSSGGSAAALAADLAPLALGSDTGGSVRQPAAFC 173
           FG+GS T +  F  T   +DP +  GGSSGG+A+ALA  L P+A GSD  GS+R PAAF 
Sbjct: 134 FGLGSQTYNPLFGATGCAYDPSKTAGGSSGGAASALAMHLVPVADGSDMMGSLRNPAAFN 193

Query: 174 GVYGLKPTYGRVS-RFGLIAYASSLDQIGPMARSVRDLALLMDAVAGPDPLDATSL-DLP 231
            V+G +P+ GRV        +   L   GPM R VRD ALL+   AG D     S+ +  
Sbjct: 194 NVFGFRPSQGRVPFDDSADLFIDQLGYEGPMGRCVRDAALLLSVQAGADARAPLSIAESG 253

Query: 232 PRFQEALEGPLPPLRLGVVREALAGNSP---GVERALEEALKVFRELGLSVREVSWPSLP 288
             F   LE      RLG + +   G  P   G+    ++A   F  LG S+   S    P
Sbjct: 254 AAFAAPLERDFSGTRLGWLGD-FNGYLPMEKGILDLCQKAFTDFESLGCSIEPASVDFAP 312

Query: 289 QAL-AAYYILAPAEASSNLARYDGTLYGRRAEGEEVEGMMEATRALFGLEVKRRVLVGTF 347
           + L  ++  L     + +L    G+ Y   ++           RA    E    V  G  
Sbjct: 313 EQLWNSWRTLRHWMVAGSL----GSAYANPSQ-----------RARLKEEACWEVENGLR 357

Query: 348 VLSSGYYEAYYGRAQAFRRRLKAEAQALFREVDLLLLPTT---------PHPAFPFGARR 398
           + +S  + A   R+  +R         LF+  D LLLPT          P P    G   
Sbjct: 358 LSASDVFAASVARSHWYR-----AISNLFKRFDYLLLPTAQVFPFDKNLPWPTSIEGVSM 412

Query: 399 DPLAMYREDLYTVGANLTGLPALSFPAGFEGH-LPVGLQLLAPWGEDERLLRAALAFEEA 457
           D    + E    +   L+G P  +   GF  + LP+GLQ++     D  +L+ A A+E+A
Sbjct: 413 DTYHRWME--VVIPGTLSGCPVANVQVGFNRNGLPMGLQIIGKHQADFAVLQLAHAYEQA 470

Query: 458 T 458
           +
Sbjct: 471 S 471


Lambda     K      H
   0.319    0.137    0.395 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 520
Number of extensions: 31
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 471
Length of database: 483
Length adjustment: 33
Effective length of query: 438
Effective length of database: 450
Effective search space:   197100
Effective search space used:   197100
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis