Alignments for a candidate for argA in Pseudomonas fluorescens FW300-N2E3

GapMind for Amino acid biosynthesis

Alignments for a candidate for argA in Pseudomonas fluorescens FW300-N2E3

Align amino-acid N-acetyltransferase (EC 2.3.1.1) (characterized)
to candidate AO353_08685 AO353_08685 N-acetylglutamate synthase

Query= BRENDA::P22567
         (432 letters)



>FitnessBrowser__pseudo3_N2E3:AO353_08685
          Length = 432

 Score =  752 bits (1942), Expect = 0.0
 Identities = 373/432 (86%), Positives = 405/432 (93%)

Query: 1   MPDYVNWLRHASPYINSHRDRTFVVMLPGEGVEHPNFGNIVHDLVLLHSLGARLVLVHGS 60
           MP+YVNWLRHASPYIN+HRD TFVVMLPG+GVEHPNFGNIVHDLVLLHSLG RLVLVHGS
Sbjct: 1   MPEYVNWLRHASPYINAHRDCTFVVMLPGDGVEHPNFGNIVHDLVLLHSLGVRLVLVHGS 60

Query: 61  RPQIEARLAARGLAPRYHRDLRVTDAPTLECVIDAVGSLRIAIEARLSMDMAASPMQGAR 120
           RPQIE RLAARGL P YH  +R+TDA TLECVIDAVG LRIAIEARLSMDMA+SPMQG+R
Sbjct: 61  RPQIETRLAARGLTPHYHHGMRITDAATLECVIDAVGQLRIAIEARLSMDMASSPMQGSR 120

Query: 121 LRVAGGNLVTARPIGVVEGVDYHHTGEVRRIDRKGIGRLLDERSIVLLSPLGYSPTGEIF 180
           LRVAGGNLVTARPIGV+EG+DYHHTGEVRR+DRKGI RLLDERSIVLLSPLGYSPTGEIF
Sbjct: 121 LRVAGGNLVTARPIGVLEGIDYHHTGEVRRVDRKGINRLLDERSIVLLSPLGYSPTGEIF 180

Query: 181 NLACEDVAMRAAIDLEAEKLILYGAEQGLLDASGKLVRELRPQQVPAHLQRLGNSYQAEL 240
           NLACEDVA RAAIDL A+KL+L+GA+ GL+D +G+LVRELRPQQVPAHLQRLG++YQAEL
Sbjct: 181 NLACEDVATRAAIDLGADKLLLFGADPGLIDENGRLVRELRPQQVPAHLQRLGSNYQAEL 240

Query: 241 LDAAAQACRAGVKRSHIVSYTEDGALLSELFTRTGNGTLVAQEQFEQLREAGIEDVGGLI 300
           LDAAA+ACR GV RSHIVSY EDGALL+ELFTR G GTLVAQEQFE +REA IEDVGGL+
Sbjct: 241 LDAAAEACRGGVGRSHIVSYAEDGALLTELFTRDGGGTLVAQEQFEVVREAAIEDVGGLL 300

Query: 301 ELIRPLEEQGILVRRSREVLEREIEQFSIVEREGLIIACAALYPIADSEAGELACLAVNP 360
           +LI PLEEQGILVRRSREVLEREIEQFS+VEREG+IIACAALY IADS+AGELACLAVNP
Sbjct: 301 DLISPLEEQGILVRRSREVLEREIEQFSVVEREGMIIACAALYQIADSDAGELACLAVNP 360

Query: 361 EYRHGGRGDELLERIEERARGLGLKTLFVLTTRTAHWFRERGFQPSSVERLPAARASLYN 420
           EYRHGGRGDELLERIE RAR  GLKTLFVLTTRTAHWFRERGF PSSVERLP+ARASLYN
Sbjct: 361 EYRHGGRGDELLERIETRARAQGLKTLFVLTTRTAHWFRERGFVPSSVERLPSARASLYN 420

Query: 421 FQRNSQVFEKSL 432
           +QRNS++FEK+L
Sbjct: 421 YQRNSKIFEKTL 432


Lambda     K      H
   0.321    0.138    0.398 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 731
Number of extensions: 15
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 432
Length of database: 432
Length adjustment: 32
Effective length of query: 400
Effective length of database: 400
Effective search space:   160000
Effective search space used:   160000
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 51 (24.3 bits)

Align candidate AO353_08685 AO353_08685 (N-acetylglutamate synthase)
to HMM TIGR01890 (argA: amino-acid N-acetyltransferase (EC 2.3.1.1))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01890.hmm
# target sequence database:        /tmp/gapView.5098.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01890  [M=429]
Accession:   TIGR01890
Description: N-Ac-Glu-synth: amino-acid N-acetyltransferase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                     Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                     -----------
   1.6e-223  728.3   0.1   1.7e-223  728.1   0.1    1.0  1  lcl|FitnessBrowser__pseudo3_N2E3:AO353_08685  AO353_08685 N-acetylglutamate sy


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__pseudo3_N2E3:AO353_08685  AO353_08685 N-acetylglutamate synthase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  728.1   0.1  1.7e-223  1.7e-223       1     429 []       4     432 .]       4     432 .] 1.00

  Alignments for each domain:
  == domain 1  score: 728.1 bits;  conditional E-value: 1.7e-223
                                     TIGR01890   1 fvkwlreaaPyinahrdktlvvglggelvedknlgklvadiallhslGvrlvlvhGarpqieerl 65 
                                                   +v+wlr+a+Pyinahrd+t+vv+l+g++ve++n+g++v+d++llhslGvrlvlvhG+rpqie rl
  lcl|FitnessBrowser__pseudo3_N2E3:AO353_08685   4 YVNWLRHASPYINAHRDCTFVVMLPGDGVEHPNFGNIVHDLVLLHSLGVRLVLVHGSRPQIETRL 68 
                                                   8**************************************************************** PP

                                     TIGR01890  66 akrgrtthyvrGlrvtdeaslelvkeaaGelrlaiearlsmslantpmagsrlsvvsGnfvtarP 130
                                                   a+rg+t+hy++G+r+td+a+le+v++a+G+lr aiearlsm++a++pm+gsrl+v+ Gn+vtarP
  lcl|FitnessBrowser__pseudo3_N2E3:AO353_08685  69 AARGLTPHYHHGMRITDAATLECVIDAVGQLRIAIEARLSMDMASSPMQGSRLRVAGGNLVTARP 133
                                                   ***************************************************************** PP

                                     TIGR01890 131 iGvveGvdyehtGevrkidaegirrlldersivllsPlgfsvtGeifnlamedvatsvaiklkad 195
                                                   iGv+eG+dy+htGevr++d++gi+rlldersivllsPlg+s+tGeifnla+edvat++ai+l+ad
  lcl|FitnessBrowser__pseudo3_N2E3:AO353_08685 134 IGVLEGIDYHHTGEVRRVDRKGINRLLDERSIVLLSPLGYSPTGEIFNLACEDVATRAAIDLGAD 198
                                                   ***************************************************************** PP

                                     TIGR01890 196 klillteedGildadGklvaelsaqeveslverleeettarllsaavkalrgGvarshlvsyaed 260
                                                   kl+l+++++G++d++G+lv+el++q+v ++++rl+++++a+ll+aa++a+rgGv rsh+vsyaed
  lcl|FitnessBrowser__pseudo3_N2E3:AO353_08685 199 KLLLFGADPGLIDENGRLVRELRPQQVPAHLQRLGSNYQAELLDAAAEACRGGVGRSHIVSYAED 263
                                                   ***************************************************************** PP

                                     TIGR01890 261 GallqelftrdGiGtlvskealesireatiddvggilelirPleeqGilvrrsrellereieefs 325
                                                   Gall+elftrdG Gtlv++e++e++rea i+dvgg+l+li+PleeqGilvrrsre+lereie+fs
  lcl|FitnessBrowser__pseudo3_N2E3:AO353_08685 264 GALLTELFTRDGGGTLVAQEQFEVVREAAIEDVGGLLDLISPLEEQGILVRRSREVLEREIEQFS 328
                                                   ***************************************************************** PP

                                     TIGR01890 326 viekdGliigcaalypyaeeevgelaclavsPeardggrGerllkhiedrarqvGlkrlfvlttr 390
                                                   v+e++G+ii+caaly +a++++gelaclav+Pe+r+ggrG++ll++ie+rar+ Glk+lfvlttr
  lcl|FitnessBrowser__pseudo3_N2E3:AO353_08685 329 VVEREGMIIACAALYQIADSDAGELACLAVNPEYRHGGRGDELLERIETRARAQGLKTLFVLTTR 393
                                                   ***************************************************************** PP

                                     TIGR01890 391 tehWfrerGfaeasvdelPearrklynyqrrskilvkkl 429
                                                   t+hWfrerGf+++sv++lP ar++lynyqr+ski++k l
  lcl|FitnessBrowser__pseudo3_N2E3:AO353_08685 394 TAHWFRERGFVPSSVERLPSARASLYNYQRNSKIFEKTL 432
                                                   ***********************************9986 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (429 nodes)
Target sequences:                          1  (432 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 11.86
//
[ok]

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis