Alignments for a candidate for gatA in Pseudomonas fluorescens FW300-N2C3

GapMind for Amino acid biosynthesis

Alignments for a candidate for gatA in Pseudomonas fluorescens FW300-N2C3

Align Glutamyl-tRNA(Gln) amidotransferase subunit A; Glu-ADT subunit A; EC 6.3.5.7 (uncharacterized)
to candidate AO356_25480 AO356_25480 amidase

Query= curated2:Q6MRL7
         (490 letters)



>FitnessBrowser__pseudo5_N2C3_1:AO356_25480
          Length = 485

 Score =  175 bits (443), Expect = 4e-48
 Identities = 152/495 (30%), Positives = 228/495 (46%), Gaps = 48/495 (9%)

Query: 11  ISEAVNNRSISAKEVTLHFLKRIENLNPKLNAFTSLNPQAVQEAEAVDARIANGEDVGLL 70
           ++E V    +   E+    ++R+E + P+LNA       + ++A A   ++  G    LL
Sbjct: 18  LAEWVRRGEVQPGELLETAIERLERVEPQLNAVAERLYDSARQA-ARTPQVGQG----LL 72

Query: 71  AGVPFGIKEMFC-TKGLTTTAGSKILENFVPPYDATAVARLKKSGIVVMGKLNQDEFAMG 129
           AGVP  IK++F    G   T GS+ L +F   +++  V RL+++G  VMG     EF   
Sbjct: 73  AGVPTLIKDLFSPVHGAAMTNGSRALGDFRADFESEVVTRLRRAGCQVMGTSTSPEFGTS 132

Query: 130 SSNETSFHGVVKNPWDLERVPGGSSGGSAAAQASRLVAGTLGTDTGGSIRQPASFCGIVG 189
            S E++  G  +NPW  E   GGSSGG+AA  A+R+V    G D GGS+R PAS CG+ G
Sbjct: 133 YSTESARFGATRNPWSSEHSAGGSSGGAAALVAARVVPFAHGNDGGGSLRVPASCCGVFG 192

Query: 190 VKPTYGRVSRYGIVAYA-SSLDQAGPMVSSVRDAALTLEVISGFDPQDSTTTQKQVPAWS 248
            KP+ G +    IV    + +     +  SVRD+A  L+  +G D         Q   ++
Sbjct: 193 FKPSRGLMPSGPIVGEGWAGMGTPHAITLSVRDSAALLDATAGMDLGAPYAAPVQALPYT 252

Query: 249 QNLKADVKGMKIGLMKEYMTGALDPDVQKTVENSVDTLKQLGAEIVEVSVP--MTAFAVP 306
             ++AD K ++I L+++       P   + V  +    + LG  +  V++P  +  F   
Sbjct: 253 MAVQADPKPLRIALVEQLGPWPTAPQSLQAVGEAARLCEALGHRVEPVNLPVGLLEFLDH 312

Query: 307 VYYLVAASEASSNLSRYDGVKYGYRAEFKNLSAVDLEEFYSQTRG---QAFGAEVKRRIM 363
           V+ ++ AS      SR+                VDL     Q RG   QA   EV+ RI+
Sbjct: 313 VFTIIGAS------SRH---------------YVDL---LGQMRGFAVQAEELEVRTRII 348

Query: 364 LGTYCLSSGYYDAFYNKAGQVRRLIMEQYLEAFKKCDVILSPVTTAPAFKIGERVSDPLA 423
           L      SG   A Y  A +    +  Q     +  DVIL+PV T     IGE     + 
Sbjct: 349 LRDKGNVSG---AQYAAAVEWIHALGRQLAVFMQDYDVILTPVLTREPVLIGELDLQDVC 405

Query: 424 MYLNDI---------FTTSTNLAGLPGMSVPFGQSQSGLPIGIQLTAGHFEEQKMLNVAF 474
           M L+ +         FT   N +G P MSVP   S +GLP+G        EE  +L +A 
Sbjct: 406 MSLDQLIERYHSYSPFTALFNASGQPAMSVPLSWSANGLPMGAHFAGRFGEENTLLALAA 465

Query: 475 ALEGASLVKGKHPHV 489
            LE A   +G+ P V
Sbjct: 466 QLERAQPWRGRVPAV 480


Lambda     K      H
   0.316    0.132    0.372 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 513
Number of extensions: 32
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 1
Length of query: 490
Length of database: 485
Length adjustment: 34
Effective length of query: 456
Effective length of database: 451
Effective search space:   205656
Effective search space used:   205656
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis