Align L-threonine aldolase (EC 4.1.2.5) (characterized)
to candidate AO356_13955 AO356_13955 threonine aldolase
Query= reanno::pseudo3_N2E3:AO353_07670 (346 letters) >FitnessBrowser__pseudo5_N2C3_1:AO356_13955 Length = 346 Score = 671 bits (1730), Expect = 0.0 Identities = 323/345 (93%), Positives = 334/345 (96%) Query: 1 MTDKSQQFASDNYSGICPEAWAAMEQANQGHQRAYGDDEWTARASDDFRKLFETDCEVFF 60 MTDKSQQFASDNYSGICPEAWAAME+ANQGHQRAYGDDEWT RA+DDFR LFETDCEVFF Sbjct: 1 MTDKSQQFASDNYSGICPEAWAAMEEANQGHQRAYGDDEWTHRAADDFRALFETDCEVFF 60 Query: 61 AFNGTAANSLALSSLCQSYHSVICSETAHVETDECGAPEFFSNGSKLLIARTENGKLTPE 120 AFNGTAANSLALSSLCQSYHSVICSETAHVETDECGAPEFFSNGSKLL+ARTENGKLTPE Sbjct: 61 AFNGTAANSLALSSLCQSYHSVICSETAHVETDECGAPEFFSNGSKLLLARTENGKLTPE 120 Query: 121 SIREIALKRQDIHYPKPRVVTLTQATEVGSIYTPEEIRAISATCKELGLNLHMDGARFSN 180 +IREIALKRQDIHYPKPRVVTLTQATEVGS+YTP+EIRAIS TCKELGLNLHMDGARFSN Sbjct: 121 AIREIALKRQDIHYPKPRVVTLTQATEVGSVYTPDEIRAISVTCKELGLNLHMDGARFSN 180 Query: 181 ACAFLNCSPADLTWKAGVDVLCFGGTKNGMAVGEAILFFNHKLAEDFDYRCKQAGQLASK 240 ACAFL CSPADLTWK GVDVLCFGGTKNGMAVGEAILFFNH LA DFDYRCKQAGQLASK Sbjct: 181 ACAFLGCSPADLTWKVGVDVLCFGGTKNGMAVGEAILFFNHDLAVDFDYRCKQAGQLASK 240 Query: 241 MRFLSAPWVGLLENDAWLKHARHANYCAQLLAELVSDIPGVELMFPVQANGVFLQLSESA 300 MRFLSAPWVGLL+NDAWLKHARHAN+CAQLLAELV+DIPGVELMFPVQANGVFLQLSE A Sbjct: 241 MRFLSAPWVGLLQNDAWLKHARHANHCAQLLAELVNDIPGVELMFPVQANGVFLQLSEPA 300 Query: 301 VAALTAKSWRFYTFIGKGGARFMCSWDTEVERVRELAADIREVMA 345 +AALTAK WRFYTFIGKGGARFMCSWDTE RVRELAADIREVM+ Sbjct: 301 IAALTAKGWRFYTFIGKGGARFMCSWDTEEARVRELAADIREVMS 345 Lambda K H 0.320 0.132 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 550 Number of extensions: 6 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 346 Length of database: 346 Length adjustment: 29 Effective length of query: 317 Effective length of database: 317 Effective search space: 100489 Effective search space used: 100489 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 49 (23.5 bits)
This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory