Aligments for a candidate for gly1 in Pseudomonas fluorescens FW300-N2C3

GapMind for Amino acid biosynthesis

Aligments for a candidate for gly1 in Pseudomonas fluorescens FW300-N2C3

Align glycine hydroxymethyltransferase (EC 2.1.2.1); low-specificity L-threonine aldolase (EC 4.1.2.48) (characterized)
to candidate AO356_16850 AO356_16850 serine hydroxymethyltransferase

Query= BRENDA::P0A825
         (417 letters)



>FitnessBrowser__pseudo5_N2C3_1:AO356_16850
          Length = 412

 Score =  494 bits (1272), Expect = e-144
 Identities = 243/412 (58%), Positives = 313/412 (75%), Gaps = 4/412 (0%)

Query: 6   MNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVMQAQGSQLTNKYAEGYPGKRYY 65
           M++ ++D  + + +++E+ RQE H+ELIASENY S  V+QAQGS LTNKYAEGYPGKRYY
Sbjct: 1   MSLQNFDPAIARLIDRERNRQETHLELIASENYVSEEVLQAQGSVLTNKYAEGYPGKRYY 60

Query: 66  GGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQANFAVYTALLEPGDTVLGMNLAHGG 125
           GGC+ VD +E LAI+RA++LF  +Y NVQPHSGSQAN AV+ A+LEPGDT+LGM+LAHGG
Sbjct: 61  GGCKVVDEIENLAIERARKLFNCEYVNVQPHSGSQANQAVFLAVLEPGDTILGMSLAHGG 120

Query: 126 HLTHGSPVNFSGKLYNIVPYGIDA-TGHIDYADLEKQAKEHKPKMIIGGFSAYSGVVDWA 184
           HLTHG+ VNFSGKLY    YG+D  T  +DY ++E  A+EH+PKMII G SAYS  +D+ 
Sbjct: 121 HLTHGASVNFSGKLYRAFSYGLDTETETLDYEEMEALAREHRPKMIIAGASAYSRTLDFQ 180

Query: 185 KMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVPHAHVVTTTTHKTLAGPRGGLILAKGG 244
           + R+I D +GAYL VDMAH AGL+AAGVYP+PV  A  +T+TTHKTL GPRGGLILAK  
Sbjct: 181 RFRKICDEVGAYLMVDMAHYAGLIAAGVYPSPVGIADFITSTTHKTLRGPRGGLILAKAQ 240

Query: 245 SEELYKKLNSAVFPGGQGGPLMHVIAGKAVALKEAMEPEFKTYQQQVAKNAKAMVEVFLE 304
              L   L+  +FP  QGGPLMHVIA KAVA  EA+   FK YQQ+V  NA+ M +V   
Sbjct: 241 YAAL---LDKTIFPVYQGGPLMHVIAAKAVAFNEALGDGFKHYQQRVIDNARVMADVLTR 297

Query: 305 RGYKVVSGGTDNHLFLVDLVDKNLTGKEADAALGRANITVNKNSVPNDPKSPFVTSGIRV 364
           RG +VVSGGTD H+FL+DL   N+TGK+A+A L  A+IT+NKN++PNDP+ P VTSGIR+
Sbjct: 298 RGLRVVSGGTDCHMFLLDLRSMNITGKDAEALLESAHITLNKNAIPNDPQKPAVTSGIRI 357

Query: 365 GTPAITRRGFKEAEAKELAGWMCDVLDSINDEAVIERIKGKVLDICARYPVY 416
           GTPA+T RGF EAE  E+A  + D+L+  ++ A ++  + +V+ +C  +PVY
Sbjct: 358 GTPALTTRGFGEAECAEVANLIADLLEQPDNAARLDNTRRRVMHLCECFPVY 409


Lambda     K      H
   0.316    0.134    0.393 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 554
Number of extensions: 22
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 417
Length of database: 412
Length adjustment: 31
Effective length of query: 386
Effective length of database: 381
Effective search space:   147066
Effective search space used:   147066
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis