Alignments for a candidate for ilvA in Pseudomonas fluorescens FW300-N2C3

GapMind for Amino acid biosynthesis

Alignments for a candidate for ilvA in Pseudomonas fluorescens FW300-N2C3

Align L-threonine ammonia-lyase; L-serine ammonia-lyase; Threonine dehydratase; EC 4.3.1.19; EC 4.3.1.17 (characterized)
to candidate AO356_27710 AO356_27710 threonine dehydratase

Query= SwissProt::Q9WYJ1
         (401 letters)



>FitnessBrowser__pseudo5_N2C3_1:AO356_27710
          Length = 333

 Score =  234 bits (598), Expect = 2e-66
 Identities = 127/304 (41%), Positives = 189/304 (62%), Gaps = 4/304 (1%)

Query: 20  RTALAYSSVLSEVTGGEIYLKMENLQKTGSFKIRGAYNKIAHLSEEERKRGVVAASAGNH 79
           RT L  +  LSE  G  I LK E+LQ T SFKIRGAYNK+ HL+ E+R RGV+ ASAGNH
Sbjct: 29  RTPLQAAPALSEALGNRILLKREDLQPTFSFKIRGAYNKLVHLTPEQRARGVITASAGNH 88

Query: 80  AQGVALAAQIFGIPATIVMPRYAPLSKITKTRNLGAQVILEGNIFDEAYEAALRIQEKTG 139
           AQGVALAA+  GI A IVMP   P  K+   RN GA+ +L G+ F  A   AL + E+TG
Sbjct: 89  AQGVALAARELGISARIVMPVTTPQLKVLGVRNRGAEALLHGDSFPFALAHALELAEQTG 148

Query: 140 AVFVHPFNDPHVIAGQGTIGLEIMEDLPD-VEVVVVPVGGGGLISGVSVAIKSMNPEVKV 198
             FV PF+DP VIAGQGT+ +EI+   P  ++ + VPVGGGGLI+G++  +K + PEV++
Sbjct: 149 REFVSPFDDPDVIAGQGTVAMEILRQHPGRLDAIFVPVGGGGLIAGIAAYVKYLRPEVRI 208

Query: 199 IGVQTENMPSMIASLRRGRAERVEGKPTLADGIAVKKPGDLTFELVKKYVDEMVAVNEEE 258
           IGV++++   + A+L  G    +    T ADG+AV + G    ++ +  VDE++ V+ ++
Sbjct: 209 IGVESQHSACLQAALAAGERVTLPEVGTFADGVAVAQIGAHGLDICRFCVDEVMTVSNDQ 268

Query: 259 IADAILFLLEQAKVVAEGAGAVGVAAV---LNKLDVKGKKVAIVISGGNIDVNMIDRIIN 315
           +  AI  + +  + + E +GA+ VA +   +     +G+ +  + SG NI+ + +  +  
Sbjct: 269 LCAAIKDIYDDTRSITEPSGALAVAGIKQYVAHTGARGETLVAIDSGANINFDSLRHVAE 328

Query: 316 KGLV 319
           +  V
Sbjct: 329 RAAV 332


Lambda     K      H
   0.317    0.136    0.367 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 279
Number of extensions: 11
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 401
Length of database: 333
Length adjustment: 30
Effective length of query: 371
Effective length of database: 303
Effective search space:   112413
Effective search space used:   112413
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 49 (23.5 bits)

Align candidate AO356_27710 AO356_27710 (threonine dehydratase)
to HMM TIGR01124 (ilvA: threonine ammonia-lyase, biosynthetic (EC 4.3.1.19))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01124.hmm
# target sequence database:        /tmp/gapView.7747.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01124  [M=499]
Accession:   TIGR01124
Description: ilvA_2Cterm: threonine ammonia-lyase, biosynthetic
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                       Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                       -----------
   5.4e-152  492.8   1.0   6.2e-152  492.6   1.0    1.0  1  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_27710  AO356_27710 threonine dehydratas


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_27710  AO356_27710 threonine dehydratase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  492.6   1.0  6.2e-152  6.2e-152       2     321 ..      14     333 .]      13     333 .] 1.00

  Alignments for each domain:
  == domain 1  score: 492.6 bits;  conditional E-value: 6.2e-152
                                       TIGR01124   2 ylrailkarvyeaavetplekaaklserlknrvllkredlqpvfsfklrGaynkmaqlsaeqk 64 
                                                     y+++il a vye av tpl+ a  lse l+nr+llkredlqp fsfk+rGaynk+ +l++eq+
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_27710  14 YVKKILAAPVYELAVRTPLQAAPALSEALGNRILLKREDLQPTFSFKIRGAYNKLVHLTPEQR 76 
                                                     899************************************************************ PP

                                       TIGR01124  65 akGviaasaGnhaqGvalsakklGvkavivmpettpeikvdavkafGgevvlhGenydeakak 127
                                                     a+Gvi+asaGnhaqGval+a++lG++a ivmp ttp++kv  v+++G+e +lhG+++  a a+
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_27710  77 ARGVITASAGNHAQGVALAARELGISARIVMPVTTPQLKVLGVRNRGAEALLHGDSFPFALAH 139
                                                     *************************************************************** PP

                                       TIGR01124 128 alelaqekgltfiapfddplviaGqGtvalellrqveedldavfvpvGGGGliaGvaalvkql 190
                                                     alela++ g  f++pfddp+viaGqGtva+e+lrq+   lda+fvpvGGGGliaG+aa+vk l
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_27710 140 ALELAEQTGREFVSPFDDPDVIAGQGTVAMEILRQHPGRLDAIFVPVGGGGLIAGIAAYVKYL 202
                                                     *************************************************************** PP

                                       TIGR01124 191 lpeikvigveaedsaalkqaleaGervkldqvGlfadGvavkevGdetfrlckeylddivlvd 253
                                                     +pe+++igve++ sa+l++al+aGerv+l +vG fadGvav ++G++ +++c+  +d++++v 
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_27710 203 RPEVRIIGVESQHSACLQAALAAGERVTLPEVGTFADGVAVAQIGAHGLDICRFCVDEVMTVS 265
                                                     *************************************************************** PP

                                       TIGR01124 254 tdevcaaikdvfedtravlepaGalalaGlkkyvakkgiedktlvailsGanlnfdrlryvse 316
                                                      d++caaikd+++dtr+++ep+Gala+aG+k+yva+ g++++tlvai sGan+nfd+lr+v+e
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_27710 266 NDQLCAAIKDIYDDTRSITEPSGALAVAGIKQYVAHTGARGETLVAIDSGANINFDSLRHVAE 328
                                                     *************************************************************** PP

                                       TIGR01124 317 raelG 321
                                                     ra +G
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_27710 329 RAAVG 333
                                                     **998 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (499 nodes)
Target sequences:                          1  (333 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01
# Mc/sec: 11.27
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis