Aligments for a candidate for leuB in Pseudomonas fluorescens FW300-N2C3

GapMind for Amino acid biosynthesis

Aligments for a candidate for leuB in Pseudomonas fluorescens FW300-N2C3

Align D-malate dehydrogenase (decarboxylating) (EC 1.1.1.83) (characterized)
to candidate AO356_26735 AO356_26735 tartrate dehydrogenase

Query= BRENDA::P76251
         (361 letters)



>lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_26735 AO356_26735 tartrate
           dehydrogenase
          Length = 359

 Score =  585 bits (1508), Expect = e-172
 Identities = 273/355 (76%), Positives = 312/355 (87%)

Query: 1   MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQMEWASCEYYSHHGKMMPD 60
           M K  RIAAI GDGIGKEVLPEG+RVL+ AA++W   LS E ++WA C+YY  HG+MMP 
Sbjct: 1   MSKPFRIAAIAGDGIGKEVLPEGLRVLEQAAKKWQLDLSIEVLDWAHCDYYLEHGQMMPA 60

Query: 61  DWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQYVNLRPVRLFPGVPCPLA 120
           DW  QL  FDAIYFGAVGWPD VPDHISLWGSLLKFRR+FDQYVN+RPVRLFPGVPCPLA
Sbjct: 61  DWFAQLKDFDAIYFGAVGWPDKVPDHISLWGSLLKFRRDFDQYVNIRPVRLFPGVPCPLA 120

Query: 121 GKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQESVFTRRGVDRILRYAFELAQS 180
           G++ GDIDF V+RENTEGEYSS+GG++ EGTEHE V+QESVFTRRGVDRIL++AF+LAQ+
Sbjct: 121 GREAGDIDFVVIRENTEGEYSSIGGKMFEGTEHEFVLQESVFTRRGVDRILKFAFDLAQT 180

Query: 181 RPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRWDKQHIDILCARFVMQPERFDVV 240
           RPRK LT+ATKSNG++ISMPYWDER   MA+ YP++ WDKQHIDILCARFV+QP+RFDVV
Sbjct: 181 RPRKRLTAATKSNGISISMPYWDERTALMAQQYPDVTWDKQHIDILCARFVLQPDRFDVV 240

Query: 241 VASNLFGDILSDLGPACTGTIGIAPSANLNPERTFPSLFEPVHGSAPDIYGKNIANPIAT 300
           VASNLFGDILSDLGPAC GTIGIAPSANL+PER FPSLFEPVHGSAPDIYG+NIANPIA 
Sbjct: 241 VASNLFGDILSDLGPACAGTIGIAPSANLDPERRFPSLFEPVHGSAPDIYGRNIANPIAM 300

Query: 301 IWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIAHGPKTPDMKGNATTPQVADAI 355
           IW+GA+MLDFLGNGDER++ AH+GIL AIE+VIA GP TPD+ G  +T  V  AI
Sbjct: 301 IWSGALMLDFLGNGDERYRAAHDGILRAIEQVIAEGPITPDLGGQGSTQDVGQAI 355


Lambda     K      H
   0.321    0.138    0.432 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 533
Number of extensions: 10
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 361
Length of database: 359
Length adjustment: 29
Effective length of query: 332
Effective length of database: 330
Effective search space:   109560
Effective search space used:   109560
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 49 (23.5 bits)

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis