Alignments for a candidate for B12-reactivation-domain in Pseudomonas fluorescens FW300-N2C3

GapMind for Amino acid biosynthesis

Alignments for a candidate for B12-reactivation-domain in Pseudomonas fluorescens FW300-N2C3

Align candidate AO356_00545 AO356_00545 (methionine synthase)
to HMM PF02965 (Met_synt_B12)

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/PF02965.21.hmm
# target sequence database:        /tmp/gapView.19413.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       Met_synt_B12  [M=273]
Accession:   PF02965.21
Description: Vitamin B12 dependent methionine synthase, activation domain
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                       Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                       -----------
     2e-125  403.8   0.0   3.3e-125  403.1   0.0    1.4  1  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_00545  AO356_00545 methionine synthase


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_00545  AO356_00545 methionine synthase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  403.1   0.0  3.3e-125  3.3e-125       1     273 []     941    1218 ..     941    1218 .. 0.96

  Alignments for each domain:
  == domain 1  score: 403.1 bits;  conditional E-value: 3.3e-125
                                    Met_synt_B12    1 dleelveyidWtpffqaWelkgkypkiledekvgeeakklfkdAqamLkkiieekllkaka 61  
                                                      dl+ l+eyidWtpff +W+l+gkyp+il+de+vge+a++l++dA+amL+k+i+ekl++a+a
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_00545  941 DLNVLAEYIDWTPFFISWDLAGKYPRILTDEVVGEAATALYADARAMLRKLIDEKLISARA 1001
                                                      58899******************************************************** PP

                                    Met_synt_B12   62 vvglfpAnse.gddievyadesrseelatlhtLrqqaekeegkpnlclaDfvapkesgvkD 121 
                                                      v+g++pAn++ +dd+evy d+   + la+lh+Lrqq  k++gkpn++laDfvapk+sgv+D
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_00545 1002 VFGFWPANQVhDDDLEVYGDDG--KPLARLHHLRQQIIKTDGKPNFSLADFVAPKDSGVTD 1060
                                                      *********94588****8877..689********************************** PP

                                    Met_synt_B12  122 yiGlFavtaglgieelakefeaekddYsailvkaladrLaeAfaellhekvrkelWgyakd 182 
                                                      y+G+F++tag+g+ee+ak+++++ ddY++i+vkaladrLaeA+ae+lh++vrke+Wgya+d
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_00545 1061 YVGGFITTAGIGAEEVAKAYQEAGDDYNSIMVKALADRLAEACAEWLHQQVRKEHWGYARD 1121
                                                      ************************************************************* PP

                                    Met_synt_B12  183 eklsneelikekYqgiRpApGYpacpdhtekktlfelldaee......kigieLteslamt 237 
                                                      e+l+ne+like+Y+giRpApGYpacpdhtek+tlf+lld e+      + g+ Lte++am 
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_00545 1122 ESLDNEALIKEQYTGIRPAPGYPACPDHTEKATLFRLLDPEAselkagRSGVFLTEHYAMF 1182
                                                      **************************************9986333333589********** PP

                                    Met_synt_B12  238 PaasvsGlyfahpearyFavgkiekdqvedyakrkg 273 
                                                      Paa+vsG+yfahp+a+yFavgki+kdqv++y++rkg
  lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_00545 1183 PAAAVSGWYFAHPQAQYFAVGKIDKDQVQSYTARKG 1218
                                                      **********************************97 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (273 nodes)
Target sequences:                          1  (1236 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 24.19
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis