Align Threonine synthase; TS; EC 4.2.3.1 (uncharacterized)
to candidate AO356_16855 AO356_16855 threonine synthase
Query= curated2:Q58860 (405 letters) >FitnessBrowser__pseudo5_N2C3_1:AO356_16855 Length = 417 Score = 221 bits (563), Expect = 3e-62 Identities = 139/381 (36%), Positives = 206/381 (54%), Gaps = 10/381 (2%) Query: 4 RCIKCGKTYDVDEIIYTC---ECGGLLEIIYDYEEIKDKVSEEKLRKREI-GVWRYLEYL 59 +C+ C ++Y E+ Y C G L+ +YDY +K + S + L G+WRY + Sbjct: 11 QCLVCDRSYQPHEVSYYCPHCNIDGALDALYDYPTLKREWSRDSLAHDHARGMWRYDRLM 70 Query: 60 PVKDESKIVSLCEGGTPLY-RCNNLEKELGIKELYVKNEGANPTGSFKDRGMTVGVTRAN 118 P+ I L G TPLY R L K IK ++VK+E PTGS KDR + V A Sbjct: 71 PLSSTQFIPPLLVGNTPLYSRPELLGKGARIK-VFVKDESRQPTGSLKDRASALAVAHAM 129 Query: 119 ELGVEVVGCASTGNTSASLAAYSARSGKKCIVLLPEGKVALGKLAQAMFYGAKVIQVKGN 178 + G V ASTGN +++LA SA G ++ LP+ KLAQ YGA+++ V G Sbjct: 130 QSGARTVAVASTGNAASALAGMSASLGLHNVIYLPKS-APREKLAQMQGYGAEIVLVDGQ 188 Query: 179 FDDALDMVKQLAKEKLIY-LLNSINPFRLEGQKTIAFEICDQLNWQVPDRVIVPVGNAGN 237 +D+A + ++ Y IN + EG+KT+AFE+C+QLNWQVPD V VPVGN Sbjct: 189 YDEAFEQCLNACEKHGWYNRTTGINSYMSEGKKTVAFEMCEQLNWQVPDLVFVPVGNGCI 248 Query: 238 ISAIWKGFKEFEITGIIDELPKMTGIQADGAKPIVEAFR-KRAKDIIPYKNPETIATAIR 296 + +++KGF + G I+ +P++ G+QA+ + + A+R R+ P ++A++I Sbjct: 249 LGSVYKGFFDLLQLGWIERIPRLIGVQAEHSNFMYRAWRHDRSMQQTERLPPTSLASSIN 308 Query: 297 IGNPVNAPKALDAIYSSGGYAEAVTDEEIVEAQKLLARKEGIFVEPASASSIAGLKKLLE 356 + P + KA+ A+ +S G V+D IV A LA G+F E +AS+ AGL K E Sbjct: 309 VALPRDRLKAMRAVTASDGEFICVSDPSIVAAASRLAASTGVFPEAGAASAFAGLLKYAE 368 Query: 357 EGIIDRDERIVCITTGHGLKD 377 D + V + TG GLKD Sbjct: 369 TH-PDTPQTAVILITGSGLKD 388 Lambda K H 0.317 0.137 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 412 Number of extensions: 27 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 405 Length of database: 417 Length adjustment: 31 Effective length of query: 374 Effective length of database: 386 Effective search space: 144364 Effective search space used: 144364 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 50 (23.9 bits)
Align candidate AO356_16855 AO356_16855 (threonine synthase)
to HMM TIGR00260 (thrC: threonine synthase (EC 4.2.3.1))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00260.hmm # target sequence database: /tmp/gapView.12901.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00260 [M=340] Accession: TIGR00260 Description: thrC: threonine synthase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 2e-55 174.2 0.0 2.3e-55 174.0 0.0 1.0 1 lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_16855 AO356_16855 threonine synthase Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_16855 AO356_16855 threonine synthase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 174.0 0.0 2.3e-55 2.3e-55 1 339 [. 63 389 .. 63 390 .. 0.92 Alignments for each domain: == domain 1 score: 174.0 bits; conditional E-value: 2.3e-55 TIGR00260 1 vvryreilevt.ekdlvdlaegstelfrspklaeevgaenlyvkelfhgPtlaFKDrglqfva 62 ++ry +++ + + + l g+t+l+ p+l + +++vk+++ Pt++ KDr+ a lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_16855 63 MWRYDRLMPLSsTQFIPPLLVGNTPLYSRPELLGKGARIKVFVKDESRQPTGSLKDRA---SA 122 699999999996666678999**********9955555599*****************...99 PP TIGR00260 63 vlltkalelgnetvlcAtsGdtgaaaaealagkanvkvvvLyPkgkispvkeklvtalaenak 125 ++++a + g+ tv++A++G+ ++a+a ++++ +++ v+ +Pk ekl++ ++a+ lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_16855 123 LAVAHAMQSGARTVAVASTGNAASALA-GMSASLGLHNVIYLPKS-AP--REKLAQMQGYGAE 181 ***************************.****************9.33..7************ PP TIGR00260 126 vlaikGdFDdaqdlvkeifedkeklklnsvNsinparieaqktyafeiveqlgkespdkvvvp 188 ++ ++G++D+a + + +++e++ ++ in + e++kt+afe++eql+++ pd v vp lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_16855 182 IVLVDGQYDEAFEQCLNACEKHGWYN--RTTGINSYMSEGKKTVAFEMCEQLNWQVPDLVFVP 242 *******************7655443..33345599*************************** PP TIGR00260 189 vp.sgnfgailkGflekkelg....lpieklaiaaegaadivrrflksgdlepkedk..eTls 244 v+ + +g+++kGf+ + +lg p + ++++ae ++ ++r+ ++++ +++ e++ l+ lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_16855 243 VGnGCILGSVYKGFFDLLQLGwierIP-RLIGVQAEHSNFMYRAWRHDRSMQQTERLppTSLA 304 **99999*****************999.*********************777777766699** PP TIGR00260 245 tAmdignpsnveralelarrslgnledlkesvsdeeileaikklaeeegyllephtavavaal 307 ++++ p + +a++++ s+g++ + sd +i+ a +la++ g++ e +a a a+l lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_16855 305 SSINVALPRDRLKAMRAVTASDGEFICV----SDPSIVAAASRLAASTGVFPEAGAASAFAGL 363 *************************999....******************************* PP TIGR00260 308 kklvekgvsatadpaKFeevvealtgnklkdp 339 +k +e +p+ +++v +tg +lkd lcl|FitnessBrowser__pseudo5_N2C3_1:AO356_16855 364 LKYAET------HPDTPQTAVILITGSGLKDT 389 **9883......6887799**********985 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (340 nodes) Target sequences: 1 (417 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 12.97 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory