Alignments for a candidate for aroG in Pseudomonas fluorescens FW300-N2E2

GapMind for Amino acid biosynthesis

Alignments for a candidate for aroG in Pseudomonas fluorescens FW300-N2E2

Align 3-deoxy-7-phosphoheptulonate synthase (EC 2.5.1.54) (characterized)
to candidate Pf6N2E2_2453 2-keto-3-deoxy-D-arabino-heptulosonate-7- phosphate synthase II (EC 2.5.1.54)

Query= BRENDA::P29976
         (525 letters)



>FitnessBrowser__pseudo6_N2E2:Pf6N2E2_2453
          Length = 449

 Score =  564 bits (1454), Expect = e-165
 Identities = 268/444 (60%), Positives = 338/444 (76%), Gaps = 1/444 (0%)

Query: 75  WTPESWKLKKALQLPDYPNANELESVLKTIEAFPPIVFAGEARNLEERLADAAVGKAFLL 134
           W+P+SW+     Q P YP+A  L  V +T+ ++PP+VFAGEAR L  + A+   G+AFLL
Sbjct: 6   WSPDSWRALPIQQQPRYPDAAHLLQVEQTLASYPPLVFAGEARELRRQFAEVTQGRAFLL 65

Query: 135 QGGDCAESFKEFNATNIRDTFRVLLQMSIVLTFGGQVPVIKVGRMAGQFAKPRSDAFEEK 194
           QGGDCAESF EF+A  IRDTF+VLLQM+IV+TF    PV+KVGRMAGQFAKPRS   E  
Sbjct: 66  QGGDCAESFAEFSAAKIRDTFKVLLQMAIVMTFAAGCPVVKVGRMAGQFAKPRSANDETI 125

Query: 195 DGVKLPSYKGDNINGDTFDEKSRIPDPNRMIRAYTQSAATLNLLRAFATGGYAAIQRVTQ 254
           DGV LP+Y+GD +NG  FDEKSR+PDP R++++Y QS ATLNLLRAFA GG+A + +V +
Sbjct: 126 DGVTLPAYRGDIVNGIGFDEKSRVPDPERLLQSYHQSTATLNLLRAFAQGGFADLHQVHK 185

Query: 255 WNLDFVEQSEQADRYQELANRVDEALGFMSACGLGTDHPLMTTTDFYTSHECLLLPYEQS 314
           WNLDF+  S  A++Y  LA+R+DE L FM ACG+ +  P +  T F+T+HE LLL YE++
Sbjct: 186 WNLDFIANSALAEKYSHLADRIDETLAFMRACGMDSS-PQLRETSFFTAHEALLLNYEEA 244

Query: 315 LTRLDSTSGLYYDCSAHMVWCGERTRQLDGAHVEFLRGIANPLGIKVSNKMDPFELVKLV 374
             R DS +  YYDCSAHM+W G+RTRQLDGAHVEFLRG+ NP+G+KV   MDP +L++L+
Sbjct: 245 FVRRDSLTNDYYDCSAHMLWIGDRTRQLDGAHVEFLRGVNNPIGVKVGPSMDPDDLIRLI 304

Query: 375 EILNPNNKPGRITVIVRMGAENMRVKLPHLIRAVRRSGQIVTWVCDPMHGNTIKAPCGLK 434
           ++LNP N PGR+ +I RMGA  +   LP L+RAV+R G+ V W  DPMHGNTIKA  G K
Sbjct: 305 DVLNPQNDPGRLNLIARMGANKVGEHLPPLLRAVQREGKQVLWSSDPMHGNTIKASSGYK 364

Query: 435 TRAFDSILAEVRAFLDVHEQEGSHAGGIHLEMTGQNVTECIGGSRTVTYDDLSSRYHTHC 494
           TR F  IL EV+ F  VHE EG++AGGIH+EMTGQNVTECIGG+R +T D LS RYHTHC
Sbjct: 365 TRDFAQILGEVKQFFQVHEAEGTYAGGIHIEMTGQNVTECIGGARPITEDGLSDRYHTHC 424

Query: 495 DPRLNASQSLELAFIVAERLRKRR 518
           DPR+NA QSLELAF++AE L++ R
Sbjct: 425 DPRMNADQSLELAFLIAETLKQVR 448


Lambda     K      H
   0.318    0.132    0.389 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 651
Number of extensions: 16
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 525
Length of database: 449
Length adjustment: 34
Effective length of query: 491
Effective length of database: 415
Effective search space:   203765
Effective search space used:   203765
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

Align candidate Pf6N2E2_2453 (2-keto-3-deoxy-D-arabino-heptulosonate-7- phosphate synthase II (EC 2.5.1.54))
to HMM TIGR01358 (3-deoxy-7-phosphoheptulonate synthase (EC 2.5.1.54))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01358.hmm
# target sequence database:        /tmp/gapView.5519.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01358  [M=443]
Accession:   TIGR01358
Description: DAHP_synth_II: 3-deoxy-7-phosphoheptulonate synthase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                      Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                      -----------
   2.3e-225  734.1   0.0   2.6e-225  733.9   0.0    1.0  1  lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_2453  2-keto-3-deoxy-D-arabino-heptulo


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_2453  2-keto-3-deoxy-D-arabino-heptulosonate-7- phosphate synthase II (EC 2.
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  733.9   0.0  2.6e-225  2.6e-225       1     443 []       6     448 ..       6     448 .. 1.00

  Alignments for each domain:
  == domain 1  score: 733.9 bits;  conditional E-value: 2.6e-225
                                      TIGR01358   1 wsleswrskpaaqlPeyPdaealeavldtleslPPlvlageilklkeklaevakGeafllqgGd 64 
                                                    ws++swr+ p +q+P yPda++l +v++tl+s+PPlv+age+++l++++aev++G+afllqgGd
  lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_2453   6 WSPDSWRALPIQQQPRYPDAAHLLQVEQTLASYPPLVFAGEARELRRQFAEVTQGRAFLLQGGD 69 
                                                    89************************************************************** PP

                                      TIGR01358  65 caesfkeveadnirdklrvllqmavvltygaslPvvkvgriaGqyakPrsepieakdgvtlpsy 128
                                                    caesf e++a++ird+++vllqma+v+t++a+ Pvvkvgr+aGq+akPrs++ e+ dgvtlp+y
  lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_2453  70 CAESFAEFSAAKIRDTFKVLLQMAIVMTFAAGCPVVKVGRMAGQFAKPRSANDETIDGVTLPAY 133
                                                    **************************************************************** PP

                                      TIGR01358 129 rGdvingaafdeaarvpdperlvrayaksaatlnllraltsgGyadlkkvheWnlefvkkspag 192
                                                    rGd++ng  fde++rvpdperl++ y++s+atlnllra+++gG+adl++vh+Wnl+f+ +s  +
  lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_2453 134 RGDIVNGIGFDEKSRVPDPERLLQSYHQSTATLNLLRAFAQGGFADLHQVHKWNLDFIANSALA 197
                                                    **************************************************************** PP

                                      TIGR01358 193 aryeklaeeidealrfmsavgvaeaealkrvelytsheallldyeealtrvdskegqlfdlsah 256
                                                    ++y +la++ide+l fm+a+g++++ +l+++ ++t+healll+yeea++r ds +++++d+sah
  lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_2453 198 EKYSHLADRIDETLAFMRACGMDSSPQLRETSFFTAHEALLLNYEEAFVRRDSLTNDYYDCSAH 261
                                                    **************************************************************** PP

                                      TIGR01358 257 llWiGertrqldgahveflrgvknPigikvgpsmeadellklievldPenePGrltlisrlGae 320
                                                    +lWiG+rtrqldgahveflrgv+nPig+kvgpsm +d+l++li+vl+P+n+PGrl li+r+Ga+
  lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_2453 262 MLWIGDRTRQLDGAHVEFLRGVNNPIGVKVGPSMDPDDLIRLIDVLNPQNDPGRLNLIARMGAN 325
                                                    **************************************************************** PP

                                      TIGR01358 321 kiaeklPelleavkaaGravvWvtdpmhGntleaasGyktrrfddilsevkeffevhkaeGthp 384
                                                    k+ e+lP+ll+av+++G++v+W +dpmhGnt++a+sGyktr f +il evk+ff+vh+aeGt+ 
  lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_2453 326 KVGEHLPPLLRAVQREGKQVLWSSDPMHGNTIKASSGYKTRDFAQILGEVKQFFQVHEAEGTYA 389
                                                    **************************************************************** PP

                                      TIGR01358 385 GGvhleltGedvteclGGareitetdlasryetacdPrlnaeqslelaflvaeklrear 443
                                                    GG+h+e+tG++vtec+GGar ite+ l++ry+t+cdPr+na+qslelafl+ae+l+++r
  lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_2453 390 GGIHIEMTGQNVTECIGGARPITEDGLSDRYHTHCDPRMNADQSLELAFLIAETLKQVR 448
                                                    *******************************************************9975 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (443 nodes)
Target sequences:                          1  (449 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.01
# Mc/sec: 10.72
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis