Align cysteine synthase (EC 2.5.1.47) (characterized)
to candidate Pf6N2E2_2700 Cysteine synthase (EC 2.5.1.47)
Query= BRENDA::P0ABK5 (323 letters) >FitnessBrowser__pseudo6_N2E2:Pf6N2E2_2700 Length = 324 Score = 447 bits (1149), Expect = e-130 Identities = 229/325 (70%), Positives = 267/325 (82%), Gaps = 3/325 (0%) Query: 1 MSKIFEDNSLTIGHTPLVRLNRIG--NGRILAKVESRNPSFSVKCRIGANMIWDAEKRGV 58 MS+IF DN+ +IG+TPLV++NRI ILAK+E RNP +SVKCRIGANMIWDAE G Sbjct: 1 MSRIFADNAHSIGNTPLVQINRIAPRGVTILAKIEGRNPGYSVKCRIGANMIWDAESSGK 60 Query: 59 LKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRKLLKALGANLVLTEGAK 118 LKPG+ +VEPTSGNTGI LA+VAAARGYKL LTMP +MSIERRK+LKALGA LVLTE AK Sbjct: 61 LKPGMTIVEPTSGNTGIGLAFVAAARGYKLMLTMPASMSIERRKVLKALGAELVLTEPAK 120 Query: 119 GMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPEIWEDTDGQVDVFIAGVGT 178 GMKGAI KA EI+AS+P KY + QQF NPANP IHEKTTGPEIW DTDG VDV +AGVGT Sbjct: 121 GMKGAIDKAAEIMASDPAKYFMPQQFDNPANPAIHEKTTGPEIWNDTDGAVDVLVAGVGT 180 Query: 179 GGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGEEIKPGPHKIQGIGAGFIPAN 238 GGT+TGVSRYIK T GK ++SVAVEP SPVI QA+AG+EIKP PHKIQGIGAGF+P N Sbjct: 181 GGTITGVSRYIKNTCGK-PILSVAVEPEVSPVITQAMAGQEIKPSPHKIQGIGAGFVPKN 239 Query: 239 LDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGAAVAAALKLQEDESFTNKNIVVI 298 LDL +VD V ++++E+ + A RLM+EEGIL GIS GAA+A A++L E K IVVI Sbjct: 240 LDLSIVDLVERVSDDESKAMALRLMQEEGILCGISCGAAMAVAVRLAEKPEMQGKTIVVI 299 Query: 299 LPSSGERYLSTALFADLFTEKELQQ 323 LP SGERYLS+ LF+DLFTE+E+QQ Sbjct: 300 LPDSGERYLSSMLFSDLFTEQEIQQ 324 Lambda K H 0.313 0.133 0.368 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 396 Number of extensions: 21 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 323 Length of database: 324 Length adjustment: 28 Effective length of query: 295 Effective length of database: 296 Effective search space: 87320 Effective search space used: 87320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 48 (23.1 bits)
Align candidate Pf6N2E2_2700 (Cysteine synthase (EC 2.5.1.47))
to HMM TIGR01139 (cysK: cysteine synthase A (EC 2.5.1.47))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR01139.hmm # target sequence database: /tmp/gapView.13468.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01139 [M=298] Accession: TIGR01139 Description: cysK: cysteine synthase A Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3.1e-139 449.3 0.5 3.5e-139 449.1 0.5 1.0 1 lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_2700 Cysteine synthase (EC 2.5.1.47) Domain annotation for each sequence (and alignments): >> lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_2700 Cysteine synthase (EC 2.5.1.47) # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 449.1 0.5 3.5e-139 3.5e-139 3 298 .] 10 313 .. 8 313 .. 0.99 Alignments for each domain: == domain 1 score: 449.1 bits; conditional E-value: 3.5e-139 TIGR01139 3 eliGntPlvrLnlaeeakaevlvkleslnPsssvkdrialamiedaekegllkkgktiveatsG 66 + iGntPlv++n++++ + +l+k+e +nP++svk+ri+++mi+dae +g+lk+g tive+tsG lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_2700 10 HSIGNTPLVQINRIAPRGVTILAKIEGRNPGYSVKCRIGANMIWDAESSGKLKPGMTIVEPTSG 73 78************************************************************** PP TIGR01139 67 ntGialamvaaargykliltmpetmslerrkllkayGaelvLtdgaegmkgaiekaeelveetp 130 ntGi+la+vaaargykl+ltmp++ms+errk+lka+GaelvLt++a+gmkgai+ka e+++++p lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_2700 74 NTGIGLAFVAAARGYKLMLTMPASMSIERRKVLKALGAELVLTEPAKGMKGAIDKAAEIMASDP 137 **************************************************************** PP TIGR01139 131 nkylllkqfenpanpeihrkttapeilkdldgkldafvagvGtGGtitGvgevlkekkp.dikv 193 ky++++qf+npanp+ih+ktt+pei++d+dg++d++vagvGtGGtitGv++++k++ + i + lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_2700 138 AKYFMPQQFDNPANPAIHEKTTGPEIWNDTDGAVDVLVAGVGTGGTITGVSRYIKNTCGkPILS 201 ********************************************************99868*** PP TIGR01139 194 vavePaespvlsgg......kpgphkiqGigagfiPkvLdkevidevikvsdeeaietarrlak 251 vaveP+ spv++++ kp phkiqGigagf+Pk+Ld +++d v +vsd+e+ ++a rl++ lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_2700 202 VAVEPEVSPVITQAmagqeiKPSPHKIQGIGAGFVPKNLDLSIVDLVERVSDDESKAMALRLMQ 265 *************9999999******************************************** PP TIGR01139 252 eeGilvGissGaavaaalkvakkle.kdkkivvilpdtgerYlstaLf 298 eeGil Gis Gaa+a a+++a+k+e ++k+ivvilpd+gerYls Lf lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_2700 266 EEGILCGISCGAAMAVAVRLAEKPEmQGKTIVVILPDSGERYLSSMLF 313 *************************9*******************998 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (298 nodes) Target sequences: 1 (324 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00 # Mc/sec: 11.58 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory