L-proline biosynthesis in Pseudomonas fluorescens FW300-N2E2

GapMind for Amino acid biosynthesis

L-proline biosynthesis in Pseudomonas fluorescens FW300-N2E2

Best path

Also see fitness data for the top candidates

Rules

Overview: Proline biosynthesis in GapMind is based on MetaCyc pathways L-proline biosynthesis I from L-glutamate (link) and III via L-ornithine aminotransferase (link). GapMind also describes proline synthesis via ornithine cyclodeaminase (PMID:11489875). Pathway II from arginine (link) should perhaps be included, but is not; it is reported in nitrogen-fixing cyanobacteria and the bacteria it was reported in also have pathway I. Pathway IV is not included because it is not reported in prokaryotes (link). Also, pathway III is described in MetaCyc as occuring only in plants, but it has been reported in the archaeon Thermococcus kodakarensis (PMC5846162).

all:

proB, proA and proC
or ornithine, OAT and proC
or ornithine and cyclodeaminase
Comment: Pathway I is proBAC. Pathway II involves ornithine aminotransferase (OAT). Some methanogens synthesize proline via ornithine cyclodeaminase (PMID:11489875).

ornithine:

argA, argB, argC, argD and argE
or argJ, argB, argC and argD
or lysW, argX, lysZ, lysY, lysJ and lysK
Comment: In L-arginine biosynthesis I, ornithine forms via acetylated intermediates, argA, and argE (link). In L-arginine biosynthesis II, ornithine forms via acetylated intermediates and argJ (link). In L-arginine biosynthesis IV, ornithine forms via LysW-modified intermediates (link).

17 steps (14 with candidates)

Or see definitions of steps

Step	Description	Best candidate	2nd candidate
proB	glutamate 5-kinase	Pf6N2E2_5103	Pf6N2E2_4993
proA	gamma-glutamylphosphate reductase	Pf6N2E2_4993
proC	pyrroline-5-carboxylate reductase	Pf6N2E2_4600
Alternative steps:
argA	N-acylglutamate synthase	Pf6N2E2_4497	Pf6N2E2_5516
argB	N-acylglutamate kinase	Pf6N2E2_4373	Pf6N2E2_4497
argC	N-acylglutamylphosphate reductase	Pf6N2E2_4836	Pf6N2E2_1484
argD	N-acetylornithine aminotransferase	Pf6N2E2_5665	Pf6N2E2_4013
argE	N-acetylornithine deacetylase	Pf6N2E2_1339	Pf6N2E2_4496
argJ	ornithine acetyltransferase	Pf6N2E2_5516
argX	glutamate--LysW ligase	Pf6N2E2_3903
cyclodeaminase	ornithine cyclodeaminase
lysJ	[LysW]-glutamate-semialdehyde aminotransferase	Pf6N2E2_2395	Pf6N2E2_5665
lysK	[LysW]-ornithine hydrolase
lysW	2-aminoadipate/glutamate carrier protein
lysY	[LysW]-glutamate-6-phosphate reductase	Pf6N2E2_4836
lysZ	[LysW]-glutamate kinase	Pf6N2E2_4373
OAT	L-ornithine 5-aminotransferase	Pf6N2E2_5326	Pf6N2E2_373

Confidence: high confidence medium confidence low confidence
? – known gap: despite the lack of a good candidate for this step, this organism (or a related organism) performs the pathway

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis