Align Indole-3-glycerol phosphate synthase; Short=IGPS; EC 4.1.1.48 (characterized, see rationale)
to candidate Pf6N2E2_4826 Indole-3-glycerol phosphate synthase (EC 4.1.1.48)
Query= uniprot:A0A166NT80_PSEFL (278 letters) >FitnessBrowser__pseudo6_N2E2:Pf6N2E2_4826 Length = 278 Score = 498 bits (1281), Expect = e-146 Identities = 256/278 (92%), Positives = 271/278 (97%) Query: 1 MSVPTVLENILARKVQEVAERSARVSLAELENLAKAADAPRGFAKALIDQAKTKQPAVIA 60 MSVPTVLE ILARK QEVAERSARVSLAELE+LA+ ADAPRGFA+ALIDQAK KQPAVIA Sbjct: 1 MSVPTVLEKILARKTQEVAERSARVSLAELESLARTADAPRGFAQALIDQAKKKQPAVIA 60 Query: 61 EIKKASPSKGVIRENFVPADIAKSYEKGGATCLSVLTDIDYFQGADAYLQQARAACKLPV 120 EIKKASPSKGVIRE+FVPA+IA+SYEKGGATCLSVLTD+D+FQGAD YL+QARAACKLPV Sbjct: 61 EIKKASPSKGVIREHFVPAEIARSYEKGGATCLSVLTDVDFFQGADDYLKQARAACKLPV 120 Query: 121 IRKDFMIDPYQIVESRALGADCVLLIVSALDDVKMAELAAVAKSVGLDVLVEVHDGDELE 180 IRKDFMIDPYQIVE+RALGADCVLLIVSALDDVKMAELAAVAKSVGLDVLVEVHDGDELE Sbjct: 121 IRKDFMIDPYQIVEARALGADCVLLIVSALDDVKMAELAAVAKSVGLDVLVEVHDGDELE 180 Query: 181 RALKTLDTPLVGVNNRNLHTFEVNLETTLDLLPRIPRDRLVITESGILNRADVELMEISD 240 RALKTLDT LVGVNNRNLHTFEV+LETTLDLLPRIPRDRLVITESGILNRADVELME+SD Sbjct: 181 RALKTLDTKLVGVNNRNLHTFEVSLETTLDLLPRIPRDRLVITESGILNRADVELMEVSD 240 Query: 241 VYAFLVGEAFMRAESPGTELQRLFFPERGIPVSGSTLD 278 VY+FLVGEAFMRAE+PG ELQRLFFPERG+PVSGSTLD Sbjct: 241 VYSFLVGEAFMRAENPGVELQRLFFPERGLPVSGSTLD 278 Lambda K H 0.318 0.135 0.369 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 363 Number of extensions: 7 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 278 Length of database: 278 Length adjustment: 25 Effective length of query: 253 Effective length of database: 253 Effective search space: 64009 Effective search space used: 64009 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory