Alignments for a candidate for IGPS in Pseudomonas fluorescens FW300-N2E2

GapMind for Amino acid biosynthesis

Alignments for a candidate for IGPS in Pseudomonas fluorescens FW300-N2E2

Align Indole-3-glycerol phosphate synthase; Short=IGPS; EC 4.1.1.48 (characterized, see rationale)
to candidate Pf6N2E2_4826 Indole-3-glycerol phosphate synthase (EC 4.1.1.48)

Query= uniprot:A0A166NT80_PSEFL
         (278 letters)



>FitnessBrowser__pseudo6_N2E2:Pf6N2E2_4826
          Length = 278

 Score =  498 bits (1281), Expect = e-146
 Identities = 256/278 (92%), Positives = 271/278 (97%)

Query: 1   MSVPTVLENILARKVQEVAERSARVSLAELENLAKAADAPRGFAKALIDQAKTKQPAVIA 60
           MSVPTVLE ILARK QEVAERSARVSLAELE+LA+ ADAPRGFA+ALIDQAK KQPAVIA
Sbjct: 1   MSVPTVLEKILARKTQEVAERSARVSLAELESLARTADAPRGFAQALIDQAKKKQPAVIA 60

Query: 61  EIKKASPSKGVIRENFVPADIAKSYEKGGATCLSVLTDIDYFQGADAYLQQARAACKLPV 120
           EIKKASPSKGVIRE+FVPA+IA+SYEKGGATCLSVLTD+D+FQGAD YL+QARAACKLPV
Sbjct: 61  EIKKASPSKGVIREHFVPAEIARSYEKGGATCLSVLTDVDFFQGADDYLKQARAACKLPV 120

Query: 121 IRKDFMIDPYQIVESRALGADCVLLIVSALDDVKMAELAAVAKSVGLDVLVEVHDGDELE 180
           IRKDFMIDPYQIVE+RALGADCVLLIVSALDDVKMAELAAVAKSVGLDVLVEVHDGDELE
Sbjct: 121 IRKDFMIDPYQIVEARALGADCVLLIVSALDDVKMAELAAVAKSVGLDVLVEVHDGDELE 180

Query: 181 RALKTLDTPLVGVNNRNLHTFEVNLETTLDLLPRIPRDRLVITESGILNRADVELMEISD 240
           RALKTLDT LVGVNNRNLHTFEV+LETTLDLLPRIPRDRLVITESGILNRADVELME+SD
Sbjct: 181 RALKTLDTKLVGVNNRNLHTFEVSLETTLDLLPRIPRDRLVITESGILNRADVELMEVSD 240

Query: 241 VYAFLVGEAFMRAESPGTELQRLFFPERGIPVSGSTLD 278
           VY+FLVGEAFMRAE+PG ELQRLFFPERG+PVSGSTLD
Sbjct: 241 VYSFLVGEAFMRAENPGVELQRLFFPERGLPVSGSTLD 278


Lambda     K      H
   0.318    0.135    0.369 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 363
Number of extensions: 7
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 278
Length of database: 278
Length adjustment: 25
Effective length of query: 253
Effective length of database: 253
Effective search space:    64009
Effective search space used:    64009
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis