Alignments for a candidate for trpD_1 in Pseudomonas fluorescens FW300-N2E2

GapMind for Amino acid biosynthesis

Alignments for a candidate for trpD_1 in Pseudomonas fluorescens FW300-N2E2

Align Aminodeoxychorismate/anthranilate synthase component 2; ADC synthase; ADCS; 4-amino-4-deoxychorismate synthase component 2; Aminodeoxychorismate synthase, glutamine amidotransferase component; EC 2.6.1.85; EC 4.1.3.27 (characterized)
to candidate Pf6N2E2_1718 Anthranilate synthase, amidotransferase component (EC 4.1.3.27)

Query= SwissProt::P28819
         (194 letters)



>FitnessBrowser__pseudo6_N2E2:Pf6N2E2_1718
          Length = 194

 Score =  181 bits (460), Expect = 6e-51
 Identities = 89/190 (46%), Positives = 127/190 (66%), Gaps = 4/190 (2%)

Query: 2   ILMIDNYDSFTYNLVQYLGELGEELVVKRND-SITIDEIEELSPDFLMISPGPCSPDEAG 60
           + +ID YDSF + + QYL +LG E  V+R+D    I  IE  SPDF ++ PGP  P + G
Sbjct: 3   VFLIDAYDSFVFIISQYLEQLGLETHVERHDVPDLIQRIEAFSPDFCVLGPGPGHPADVG 62

Query: 61  ISLEAIKHFAGKIPIFGVCLGHQSIAQVFGGDVVRAERLMHGKTSDIEHDGKTIFEGLKN 120
             +E I+HF G++P+ GVCLGHQ+I   FG  V RA  +MHGK S IE+DGK +++  + 
Sbjct: 63  Y-IEVIRHFQGRLPLLGVCLGHQAIGLAFGAQVCRAPHVMHGKVSTIENDGKGVYDHTQA 121

Query: 121 PLV-ATRYHSLIVKPETLPSCFTVTAQ-TKEGEIMAIRHNDLPIEGVQFHPESIMTSFGK 178
             + ATRYHSL++  + LP C  +T++ T +G +M +RH  LP+EGVQFHPESI+T  G 
Sbjct: 122 QAIRATRYHSLMISEQPLPDCLEITSRSTDDGYVMGVRHRHLPVEGVQFHPESILTENGL 181

Query: 179 EMLRNFIETY 188
           ++ R+FI  +
Sbjct: 182 DLFRSFIRCH 191


Lambda     K      H
   0.320    0.139    0.404 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 137
Number of extensions: 7
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 194
Length of database: 194
Length adjustment: 20
Effective length of query: 174
Effective length of database: 174
Effective search space:    30276
Effective search space used:    30276
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 44 (21.6 bits)

Align candidate Pf6N2E2_1718 (Anthranilate synthase, amidotransferase component (EC 4.1.3.27))
to HMM TIGR00566 (glutamine amidotransferase of anthranilate synthase or aminodeoxychorismate synthase)

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00566.hmm
# target sequence database:        /tmp/gapView.22508.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00566  [M=192]
Accession:   TIGR00566
Description: trpG_papA: glutamine amidotransferase of anthranilate synthase or aminodeoxychorismate synthase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                      Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                      -----------
    4.5e-66  208.3   0.0    5.2e-66  208.1   0.0    1.0  1  lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_1718  Anthranilate synthase, amidotran


Domain annotation for each sequence (and alignments):
>> lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_1718  Anthranilate synthase, amidotransferase component (EC 4.1.3.27)
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  208.1   0.0   5.2e-66   5.2e-66       2     191 ..       3     189 ..       2     190 .. 0.96

  Alignments for each domain:
  == domain 1  score: 208.1 bits;  conditional E-value: 5.2e-66
                                      TIGR00566   2 vllidnydsftynlvqlleelgaevvvkrnd.sltlqeieallpllsivisPGPctPdeaaiss 64 
                                                    v+lid ydsf + + q+le+lg e +v+r d ++ +q iea++p++  v++PGP++P++ +   
  lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_1718   3 VFLIDAYDSFVFIISQYLEQLGLETHVERHDvPDLIQRIEAFSPDF-CVLGPGPGHPADVGYI- 64 
                                                    89***************************9945889**********.9**********99976. PP

                                      TIGR00566  65 leliehlaGklPilGvClGhqalaqafGadvvraekvkhGkvseiehngaavfaglfnPdalka 128
                                                     e+i+h+ G+lP+lGvClGhqa+++afGa+v ra +v+hGkvs ie +g++v+   +   a++a
  lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_1718  65 -EVIRHFQGRLPLLGVCLGHQAIGLAFGAQVCRAPHVMHGKVSTIENDGKGVYDHTQAQ-AIRA 126
                                                    .***************************************************9887665.9*** PP

                                      TIGR00566 129 tryhslvveaetldtllevtaleeeeieimairhrdlpleGvqfhPesilselGkellanflk 191
                                                    tryhsl++  + l+++le+t+ +  +  +m++rhr+lp+eGvqfhPesil+e+G  l + f++
  lcl|FitnessBrowser__pseudo6_N2E2:Pf6N2E2_1718 127 TRYHSLMISEQPLPDCLEITSRSTDDGYVMGVRHRHLPVEGVQFHPESILTENGLDLFRSFIR 189
                                                    ***********************************************************9996 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (192 nodes)
Target sequences:                          1  (194 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00
# Mc/sec: 6.22
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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Candidates (tab-delimited)
Steps (tab-delimited)
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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis