Alignments for a candidate for prpE in Desulfovibrio vulgaris Hildenborough

GapMind for Amino acid biosynthesis

Alignments for a candidate for prpE in Desulfovibrio vulgaris Hildenborough

Align Acyl-coenzyme A synthetase ACSM3, mitochondrial; Acyl-CoA synthetase medium-chain family member 3; Butyrate--CoA ligase 3; Butyryl-coenzyme A synthetase 3; Middle-chain acyl-CoA synthetase 3; Propionate--CoA ligase; Protein SA homolog; EC 6.2.1.2; EC 6.2.1.17 (characterized)
to candidate 207735 DVU2250 AMP-binding protein

Query= SwissProt::Q3UNX5
         (580 letters)



>MicrobesOnline__882:207735
          Length = 551

 Score =  446 bits (1147), Expect = e-129
 Identities = 236/552 (42%), Positives = 341/552 (61%), Gaps = 20/552 (3%)

Query: 34  FSNYESMKQDFKIEIPEYFNFAKDVLDQWTNMEKAGKRLSNPAFWWIDGNGEELRWSFEE 93
           F++Y+   + F ++ PE FNFA DVLD   N  +   RL   A   +D  G    ++F  
Sbjct: 6   FASYDEFLRVFSLDAPETFNFAFDVLDAMAN--ETPDRL---AIAHVDDAGVRRDYTFAW 60

Query: 94  LGLLSRKFANILTEACSLQRGDRVMVILPKIPEWWLANVACLRTGTVLIPGTTQLTQKDI 153
           L   S + AN L +A  +++G RVM++L +  E+W++ +A  R G V IP   QLT KDI
Sbjct: 61  LADASARLANAL-KARGVRKGHRVMLVLHRRIEFWVSMLALHRLGAVAIPAPAQLTPKDI 119

Query: 154 LYRLQSSKAKCIITDDTLAPAVDAVAAKCENLHSKLIVSQHSR--EGWGNLKEMMKYASD 211
           ++R++ ++ + +I DD +   ++A    C  L S L+    +   +GW + + +   AS 
Sbjct: 120 VFRVERAQVRAVIVDDGITDRIEAARPDCPTL-SVLVQCGGTPLPDGWCDYEALCADASP 178

Query: 212 SHTCVDTKHDEM------MAIYFTSGTTGPPKMIGHTHSSFGLGLSVNGRFWLDLIASDV 265
           S     T  DE+      + I+F+SGTTG PKM+ H H+ + LG  V G +W DL   D+
Sbjct: 179 SFPR-PTAPDELACGEDPLLIFFSSGTTGMPKMVEHVHT-YPLGHLVTGMYWHDLRPGDL 236

Query: 266 MWNTSDTGWAKSAWSSVFSPWTQGACVFAH-YLPRFESTSILQTLSKFPITVFCSAPTAY 324
               +DTGW K+ W   +  W  GA VF + +  +F+  ++L  ++K  +T FC+ PT Y
Sbjct: 237 HLTVADTGWGKAVWGKFYGQWMAGAAVFVYDFRGKFDPEALLDVVAKNGVTTFCAPPTVY 296

Query: 325 RMLVQNDMSSYKFNSLKHCVSAGEPINPEVMEQWRKKTGLDIYEGYGQTETVLICGNFKG 384
           R LV+ D+S Y  + L+HC +AGE +N  V   W+  TGL IYEGYGQTET L       
Sbjct: 297 RFLVRADLSRYDLSKLRHCTTAGELLNESVFHGWKAATGLSIYEGYGQTETTLQIATLSC 356

Query: 385 MKIKPGSMGKPSPAFDVKILDENGATLPPGQEGDIALQVLPERPFGLFTHYVDNPSKTAS 444
           M+ KPGS+G+P P + + +LD  G   P G+EG+I +++    P GLF  YV++  KTAS
Sbjct: 357 MEAKPGSIGRPMPGWGITLLDHEGKECPTGEEGEICIRISDGLPVGLFRGYVEDAEKTAS 416

Query: 445 TLRGSFYITGDRGYMDEDGYFWFVARSDDIILSSGYRIGPFEVESALIEHPSIAESAVVS 504
            +   +Y TGD+ +MDEDGY WF+ R DD+I SSGYRIGPFEVESAL+ HP++ E+AV  
Sbjct: 417 VMFDGYYHTGDKAWMDEDGYLWFLGRVDDLIKSSGYRIGPFEVESALVAHPAVVEAAVTG 476

Query: 505 SPDPIRGEVVKAFIVLNPDYKSHDQEQLKKEIQEHVKKTTAPYKYPRKVEFIEELPKTVS 564
            PDP+RG+ VKA IVL   Y++   E L KE+Q+HVKK TAPYKYPR VE++ ELPKT+S
Sbjct: 477 VPDPLRGQAVKATIVLAAGYEA--GEVLTKELQDHVKKVTAPYKYPRIVEYVAELPKTIS 534

Query: 565 GKVKRNELRKKE 576
           GK+KR E+R+++
Sbjct: 535 GKIKRAEIRQRD 546


Lambda     K      H
   0.319    0.134    0.416 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 864
Number of extensions: 35
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 580
Length of database: 551
Length adjustment: 36
Effective length of query: 544
Effective length of database: 515
Effective search space:   280160
Effective search space used:   280160
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis