GapMind for Amino acid biosynthesis

 

Alignments for a candidate for prpE in Desulfovibrio vulgaris Hildenborough

Align Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 (characterized)
to candidate 208482 DVU2969 acetoacetyl-CoA synthase

Query= SwissProt::P27550
         (652 letters)



>MicrobesOnline__882:208482
          Length = 661

 Score =  746 bits (1927), Expect = 0.0
 Identities = 367/623 (58%), Positives = 462/623 (74%), Gaps = 8/623 (1%)

Query: 25  EAMYQQSINVPDTFWGEQG-KILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLD 83
           EA+ +++   P+ FWGE+  +++DW KP+  V +         I+W++ G LN+A NCLD
Sbjct: 35  EALVRRAAEDPEGFWGERAAQLIDWFKPWDTVLDADM--NEPRIEWFKGGRLNVAHNCLD 92

Query: 84  RHLQENG-DRTAIIWEGDDASQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMV 142
           RH+  N  ++ AIIW+G+     + ++Y+ L+ +V RFA  L ++G+ KGD V++YMPM+
Sbjct: 93  RHVAGNRRNKAAIIWQGEPEEDVRVLTYQMLYDEVRRFAAVLRKMGVHKGDRVSLYMPMI 152

Query: 143 PEAAVAMLACARIGAVHSVIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNV 202
           PE AVAMLACARIGAVHS++F GFS  ++  RI D  +++V+T+D  +RAGR IPLK NV
Sbjct: 153 PELAVAMLACARIGAVHSIVFAGFSAVSLQNRIHDCEAKVVVTADAVLRAGRRIPLKVNV 212

Query: 203 DDALKNPNVTSVEHVVVLKRTGGKIDWQEGRDLWWHDLVEQAS--DQHQAEEMNAEDPLF 260
           D+A++     SVE VVV+ R   ++  +EGRDLWWH+++   +       EEM+AED LF
Sbjct: 213 DEAVRQ--CPSVEKVVVVNRGSLEVTMEEGRDLWWHEVMADRTLDVDRPCEEMDAEDMLF 270

Query: 261 ILYTSGSTGKPKGVLHTTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGP 320
           ILYTSGSTGKPKGV+HTTGGYL YAA T ++VFD    D+YWCTAD+GW+TGHSY++YGP
Sbjct: 271 ILYTSGSTGKPKGVVHTTGGYLTYAAHTTQWVFDVQDDDVYWCTADIGWITGHSYIVYGP 330

Query: 321 LACGATTLMFEGVPNWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSL 380
           LA GAT+LMFEGVP+WP+P R  ++V+K +VNI YTAPT +RALM EG    E  D SSL
Sbjct: 331 LALGATSLMFEGVPSWPSPDRFWRIVEKFRVNIFYTAPTVVRALMREGTDWTERHDLSSL 390

Query: 381 RILGSVGEPINPEAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRP 440
           R+LGSVGEPINPEAW WY   IG  + P+VDTWWQTETGG MI+ LP AT LK GSAT+P
Sbjct: 391 RVLGSVGEPINPEAWMWYHTHIGKGRLPIVDTWWQTETGGIMISGLPYATTLKPGSATQP 450

Query: 441 FFGVQPALVDNEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGD 500
             GV  A+V  +G+P      G LVI   WPG  R +FG  ER+  TYF  F  MY SGD
Sbjct: 451 LPGVDAAIVRPDGSPAGPNEGGHLVIRKPWPGMLRGIFGSPERYRSTYFERFPGMYESGD 510

Query: 501 GARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIY 560
           GAR D DGY+WI GR+DDV+NVSGHR+GTAE+ESALVAHP +AEAAVVG+PH +KG+AIY
Sbjct: 511 GARTDTDGYFWIMGRLDDVINVSGHRMGTAEVESALVAHPSVAEAAVVGMPHAVKGEAIY 570

Query: 561 AYVTLNHGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAA 620
           AYVTL    E + EL AE+R WVRKEIGP+ATPDVL + + LPKTRSGKIMRRILRKIAA
Sbjct: 571 AYVTLGADAEETEELRAELRAWVRKEIGPIATPDVLQFAEGLPKTRSGKIMRRILRKIAA 630

Query: 621 GDTSNLGDTSTLADPGVVEKLLE 643
           G TS  GDTSTLADPGVV  L+E
Sbjct: 631 GATSEFGDTSTLADPGVVSDLIE 653


Lambda     K      H
   0.317    0.135    0.421 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1389
Number of extensions: 64
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 652
Length of database: 661
Length adjustment: 38
Effective length of query: 614
Effective length of database: 623
Effective search space:   382522
Effective search space used:   382522
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory