GapMind for Amino acid biosynthesis

 

Aligments for a candidate for dapF in Desulfovibrio vulgaris Hildenborough

Align Diaminopimelate epimerase; DAP epimerase; EC 5.1.1.7; PLP-independent amino acid racemase (uncharacterized)
to candidate 207333 DVU1867 diaminopimelate epimerase

Query= curated2:O67693
         (279 letters)



>lcl|MicrobesOnline__882:207333 DVU1867 diaminopimelate epimerase
          Length = 282

 Score =  206 bits (523), Expect = 6e-58
 Identities = 120/277 (43%), Positives = 155/277 (55%), Gaps = 11/277 (3%)

Query: 3   FWKLQGSGNDFVVIDDRDEKLESFLKERGVSKEDFVRKVCAFHTGVGADGLILIKN-PDN 61
           F KL G GNDFV ID+R  KL         +  D+ R +C    GVGADGL+ +   P  
Sbjct: 10  FHKLHGCGNDFVFIDNRHLKLSV------EAMPDWARSICRRAFGVGADGLVFLDTAPQG 63

Query: 62  PENDFKWEFFNSDGSVAEMCGNGSRCAVRFAYERGIVGNKVRFETLAGVIKAEVYENGRK 121
            E D+ W F+N+DGS AEMCGN SRCA   A + G  G +  F T AG++ A        
Sbjct: 64  HEADYIWHFYNADGSRAEMCGNASRCAAVLAVDLGFAGPRHAFGTDAGIVHAVADVEAGY 123

Query: 122 VKVQLTPPSK-PEEKTLTVDGEEVIGVFINTGVPHFVVPVEDVEKVNVIKLGRAIRFHEE 180
            KV+LT P       TL ++G      F+NTGVPH VV  + V+ +++ +LG A+R+H  
Sbjct: 124 AKVELTRPRDLAAGTTLELEGTPFTVHFVNTGVPHAVVFSDSVDGLDLRRLGAALRYHPH 183

Query: 181 FQPKGTNVNFVQPVSEDTIKVRTYERGVESETLACGTGATACAIVSYLKGLVKKKPVNVL 240
           F P GTN NF   +   TI +RTYERGVE ET ACGTGA A A +++  GL     V V 
Sbjct: 184 FSPAGTNANFASIIDRRTIHLRTYERGVEDETYACGTGAAATAFIAHTLGLTDAS-VGVR 242

Query: 241 TRSGEVLTIDFSEDLKEVFLTGSVYKVFEGRLSEEVL 277
           T  GEVL ID  +    +FL+G   +VF G +  E L
Sbjct: 243 TSGGEVLGIDIEDG--SIFLSGKAVRVFSGEMHPEGL 277


Lambda     K      H
   0.317    0.138    0.399 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 207
Number of extensions: 8
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 279
Length of database: 282
Length adjustment: 26
Effective length of query: 253
Effective length of database: 256
Effective search space:    64768
Effective search space used:    64768
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 47 (22.7 bits)

Align candidate 207333 DVU1867 (diaminopimelate epimerase)
to HMM TIGR00652 (dapF: diaminopimelate epimerase (EC 5.1.1.7))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00652.hmm
# target sequence database:        /tmp/gapView.6230.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00652  [M=270]
Accession:   TIGR00652
Description: DapF: diaminopimelate epimerase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                       Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                       -----------
    6.4e-76  241.3   0.0    7.3e-76  241.1   0.0    1.0  1  lcl|MicrobesOnline__882:207333  DVU1867 diaminopimelate epimeras


Domain annotation for each sequence (and alignments):
>> lcl|MicrobesOnline__882:207333  DVU1867 diaminopimelate epimerase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  241.1   0.0   7.3e-76   7.3e-76       3     268 ..      10     272 ..       8     274 .. 0.93

  Alignments for each domain:
  == domain 1  score: 241.1 bits;  conditional E-value: 7.3e-76
                       TIGR00652   3 FlkmhGlgNdFvlvdevdeelvkee.aelvrkvcdrhtgvgaDgvllvep..sseeadvklrifNsDGSeaemCGNgiR 78 
                                     F k+hG gNdFv +d+++ +l  e  ++ +r++c+r +gvgaDg++++ +  + +ead+ ++++N+DGS+aemCGN++R
  lcl|MicrobesOnline__882:207333  10 FHKLHGCGNDFVFIDNRHLKLSVEAmPDWARSICRRAFGVGADGLVFLDTapQGHEADYIWHFYNADGSRAEMCGNASR 88 
                                     99****************77666666**********************9988899************************ PP

                       TIGR00652  79 cfakfvyekglkekkelsvetlaglikveveeenkkvkvdmgepkfkkeeipltvekeeekeellalevlvvdvGnPHl 157
                                     c+a  +++ g +   ++ + t+ag++++    e    kv++++p+  ++ ++l+ e++    ++ +     v++G+PH+
  lcl|MicrobesOnline__882:207333  89 CAAVLAVDLGFAG-PRHAFGTDAGIVHAVADVEAGYAKVELTRPRDLAAGTTLELEGTPF--TVHF-----VNTGVPHA 159
                                     ************7.9***********9999888889********9999999999866666..4444.....99****** PP

                       TIGR00652 158 vvfvedvekldleelgklleaheef.pegvNvefvevkkedeiklrvyERG.ageTlaCGtGavAsavvalklgktkkk 234
                                     vvf ++v++ldl +lg++l++h++f p+g+N +f+ + ++ +i+lr+yERG ++eT aCGtGa+A+a +a++lg+t+ +
  lcl|MicrobesOnline__882:207333 160 VVFSDSVDGLDLRRLGAALRYHPHFsPAGTNANFASIIDRRTIHLRTYERGvEDETYACGTGAAATAFIAHTLGLTDAS 238
                                     ******************************************************************************* PP

                       TIGR00652 235 vtvhlegge.LeievkedgkvyltGpavlvlegel 268
                                     v v ++gge L i+++ dg+++l G+av+v++ge+
  lcl|MicrobesOnline__882:207333 239 VGVRTSGGEvLGIDIE-DGSIFLSGKAVRVFSGEM 272
                                     ********62567765.557*************97 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (270 nodes)
Target sequences:                          1  (282 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 11.07
//
[ok]

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory