Aligments for a candidate for metH in Desulfovibrio vulgaris Hildenborough

GapMind for Amino acid biosynthesis

Aligments for a candidate for metH in Desulfovibrio vulgaris Hildenborough

Align 5-methyltetrahydrofolate--homocysteine methyltransferase; EC 2.1.1.13 (characterized, see rationale)
to candidate 207036 DVU1585 vitamin B12-dependent methionine synthase family protein

Query= uniprot:Q72BP9_DESVH
         (804 letters)



>lcl|MicrobesOnline__882:207036 DVU1585 vitamin B12-dependent
           methionine synthase family protein
          Length = 804

 Score = 1555 bits (4027), Expect = 0.0
 Identities = 804/804 (100%), Positives = 804/804 (100%)

Query: 1   MPDFRQALRAGRRLLFDGGLGTMLQARGLPAGVSPEQFCLDRPDVLRGIHADYVRAGADI 60
           MPDFRQALRAGRRLLFDGGLGTMLQARGLPAGVSPEQFCLDRPDVLRGIHADYVRAGADI
Sbjct: 1   MPDFRQALRAGRRLLFDGGLGTMLQARGLPAGVSPEQFCLDRPDVLRGIHADYVRAGADI 60

Query: 61  LTTNTFGGSRFKLGDGFDVVDFNRRMAAIAREAADASGRQAFVAGSIGPTGHFVKPLGEV 120
           LTTNTFGGSRFKLGDGFDVVDFNRRMAAIAREAADASGRQAFVAGSIGPTGHFVKPLGEV
Sbjct: 61  LTTNTFGGSRFKLGDGFDVVDFNRRMAAIAREAADASGRQAFVAGSIGPTGHFVKPLGEV 120

Query: 121 EPAALVAAFREQVRGLVAGGADLLMIETQFDLAEARAAVVAARAECSLPIAVSMTFENGV 180
           EPAALVAAFREQVRGLVAGGADLLMIETQFDLAEARAAVVAARAECSLPIAVSMTFENGV
Sbjct: 121 EPAALVAAFREQVRGLVAGGADLLMIETQFDLAEARAAVVAARAECSLPIAVSMTFENGV 180

Query: 181 SLTGSTPEVFVATMLNLGVDLLGTNCSAGPDQMHDVVASLLASASVPVLVEPNAGLPELI 240
           SLTGSTPEVFVATMLNLGVDLLGTNCSAGPDQMHDVVASLLASASVPVLVEPNAGLPELI
Sbjct: 181 SLTGSTPEVFVATMLNLGVDLLGTNCSAGPDQMHDVVASLLASASVPVLVEPNAGLPELI 240

Query: 241 DGKTVFRLPPAPFAEKTAAFAAMGARVLGGCCGTTPDHIAALRQAVADIPATLPVDSGVG 300
           DGKTVFRLPPAPFAEKTAAFAAMGARVLGGCCGTTPDHIAALRQAVADIPATLPVDSGVG
Sbjct: 241 DGKTVFRLPPAPFAEKTAAFAAMGARVLGGCCGTTPDHIAALRQAVADIPATLPVDSGVG 300

Query: 301 IVLTTRSHLVRVGGDAPVRIIGERINPTGKKQLIAELQAGDFSLALRFSDEQVEAGAPIL 360
           IVLTTRSHLVRVGGDAPVRIIGERINPTGKKQLIAELQAGDFSLALRFSDEQVEAGAPIL
Sbjct: 301 IVLTTRSHLVRVGGDAPVRIIGERINPTGKKQLIAELQAGDFSLALRFSDEQVEAGAPIL 360

Query: 361 DVNVGAPMVDEEVLLPDLVQRLITRHGVPLSIDSSNAAAIERALPYCPGSTLVNSISGEP 420
           DVNVGAPMVDEEVLLPDLVQRLITRHGVPLSIDSSNAAAIERALPYCPGSTLVNSISGEP
Sbjct: 361 DVNVGAPMVDEEVLLPDLVQRLITRHGVPLSIDSSNAAAIERALPYCPGSTLVNSISGEP 420

Query: 421 GRMERLGPLCRDHGAPFILLPLKGRKLPVAATERIAIIEELLVQAEGLGIPRRLVMVDVL 480
           GRMERLGPLCRDHGAPFILLPLKGRKLPVAATERIAIIEELLVQAEGLGIPRRLVMVDVL
Sbjct: 421 GRMERLGPLCRDHGAPFILLPLKGRKLPVAATERIAIIEELLVQAEGLGIPRRLVMVDVL 480

Query: 481 ALAVSSKAEAARQCLETIRWCTANGFATTIGLSNISFGLPARELLNGTFLAMAAGAGLSS 540
           ALAVSSKAEAARQCLETIRWCTANGFATTIGLSNISFGLPARELLNGTFLAMAAGAGLSS
Sbjct: 481 ALAVSSKAEAARQCLETIRWCTANGFATTIGLSNISFGLPARELLNGTFLAMAAGAGLSS 540

Query: 541 CIAHPGNGRIRETVACADVLLARDANAERFIDAYAAWTPATQGGPVASGPGLASQPPATT 600
           CIAHPGNGRIRETVACADVLLARDANAERFIDAYAAWTPATQGGPVASGPGLASQPPATT
Sbjct: 541 CIAHPGNGRIRETVACADVLLARDANAERFIDAYAAWTPATQGGPVASGPGLASQPPATT 600

Query: 601 LEEAVVKGDRDGVTAIVESELAAGADPFDLVQTKLIPAINEVGVKYERREYFLPQLIRSA 660
           LEEAVVKGDRDGVTAIVESELAAGADPFDLVQTKLIPAINEVGVKYERREYFLPQLIRSA
Sbjct: 601 LEEAVVKGDRDGVTAIVESELAAGADPFDLVQTKLIPAINEVGVKYERREYFLPQLIRSA 660

Query: 661 ETMQTAFRRLQPLLEEMRGAEVRPVIIMATVEGDIHDIGKNIVSLMLGNHGFEVVDLGKD 720
           ETMQTAFRRLQPLLEEMRGAEVRPVIIMATVEGDIHDIGKNIVSLMLGNHGFEVVDLGKD
Sbjct: 661 ETMQTAFRRLQPLLEEMRGAEVRPVIIMATVEGDIHDIGKNIVSLMLGNHGFEVVDLGKD 720

Query: 721 VKAETIVDAAETHGARIIGLSALMTTTMVRMEDTVKLVRARGLDVKVIVGGAVVTKAFAD 780
           VKAETIVDAAETHGARIIGLSALMTTTMVRMEDTVKLVRARGLDVKVIVGGAVVTKAFAD
Sbjct: 721 VKAETIVDAAETHGARIIGLSALMTTTMVRMEDTVKLVRARGLDVKVIVGGAVVTKAFAD 780

Query: 781 AIGADGYSADAVEAVRLAKSLLAG 804
           AIGADGYSADAVEAVRLAKSLLAG
Sbjct: 781 AIGADGYSADAVEAVRLAKSLLAG 804


Lambda     K      H
   0.320    0.137    0.392 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 2161
Number of extensions: 86
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 804
Length of database: 804
Length adjustment: 41
Effective length of query: 763
Effective length of database: 763
Effective search space:   582169
Effective search space used:   582169
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 56 (26.2 bits)

This GapMind analysis is from Aug 03 2021. The underlying query database was built on Aug 03 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, the paper from 2022 on GapMind for carbon sources, or view the source code, or see changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis