Align Argininosuccinate synthase; Citrulline--aspartate ligase; EC 6.3.4.5 (characterized)
to candidate WP_011383538.1 AMB_RS05720 argininosuccinate synthase
Query= SwissProt::P59846 (400 letters) >NCBI__GCF_000009985.1:WP_011383538.1 Length = 404 Score = 503 bits (1296), Expect = e-147 Identities = 248/392 (63%), Positives = 305/392 (77%), Gaps = 4/392 (1%) Query: 2 KIVLAYSGGLDTSIILKWLKETYRAEVIAFTADIGQGEEVEEAREKALRTGASKAIAL-- 59 K+VLAYSGGLDTSIIL+WL++ Y+ EV+ FTAD+GQGEE+E AR KA G + Sbjct: 7 KVVLAYSGGLDTSIILRWLQDQYQCEVVTFTADLGQGEELEPARAKARLMGIKEENIFID 66 Query: 60 DLKEEFVRDFVFPMMRAGAVYEGYYLLGTSIARPLIAKHLVRIAEEEGAEAIAHGATGKG 119 DL+EEFVRDFVFPM RA A+YEG YLLGTSIARPLI+K + IA GA+A++HGATGKG Sbjct: 67 DLREEFVRDFVFPMFRANALYEGVYLLGTSIARPLISKRQIEIANMVGADAVSHGATGKG 126 Query: 120 NDQVRFELTAYALKPDIKVIAPWREWSFQGRKEMIAYAEAHGIPVPVTQ--EKPYSMDAN 177 NDQVRFE+ YALKPDIKVIAPWR W R +++ +AE H IP+ + E PYS DAN Sbjct: 127 NDQVRFEMGYYALKPDIKVIAPWRLWDLTSRTKLLDFAEKHQIPIAKDKRGEAPYSTDAN 186 Query: 178 LLHISYEGGVLEDPWAEPPKGMFRMTQDPEEAPDAPEYVEVEFFEGDPVAVNGERLSPAA 237 LLHISYEG LEDPW EP + M+ + PE APD P Y+E+EF +GD VA++G RLSPAA Sbjct: 187 LLHISYEGKALEDPWVEPFEDMYTRSVAPENAPDKPTYIEIEFEKGDAVAIDGVRLSPAA 246 Query: 238 LLQRLNEIGGRHGVGRVDIVENRFVGMKSRGVYETPGGTILYHARRAVESLTLDREVLHQ 297 LL +LNE+GG +G+GR+D+VENRFVGMKSRGVYETPGG++L A RA+ESLTLDR H Sbjct: 247 LLTKLNELGGANGIGRLDLVENRFVGMKSRGVYETPGGSVLIVAHRAMESLTLDRGESHL 306 Query: 298 RDMLSPKYAELVYYGFWYAPEREALQAYFDHVARSVTGVARLKLYKGNVYVVGRKAPKSL 357 +D L P+YAEL+Y GFW+APER A+Q D V+ +V+G RLKL+KG VVGRK+PKSL Sbjct: 307 KDELMPRYAELIYNGFWFAPERIAIQTMIDKVSENVSGTVRLKLFKGVASVVGRKSPKSL 366 Query: 358 YRQDLVSFDEAGGYDQKDAEGFIKIQALRLRV 389 YR D V+F+E YDQ+DA+GFIK+ ALR+R+ Sbjct: 367 YRMDYVTFEEDSVYDQRDAQGFIKLNALRMRL 398 Lambda K H 0.319 0.137 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 493 Number of extensions: 15 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 400 Length of database: 404 Length adjustment: 31 Effective length of query: 369 Effective length of database: 373 Effective search space: 137637 Effective search space used: 137637 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 50 (23.9 bits)
Align candidate WP_011383538.1 AMB_RS05720 (argininosuccinate synthase)
to HMM TIGR00032 (argG: argininosuccinate synthase (EC 6.3.4.5))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/TIGR00032.hmm # target sequence database: /tmp/gapView.15703.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR00032 [M=394] Accession: TIGR00032 Description: argG: argininosuccinate synthase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1.5e-144 468.1 0.0 1.8e-144 467.8 0.0 1.0 1 lcl|NCBI__GCF_000009985.1:WP_011383538.1 AMB_RS05720 argininosuccinate sy Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000009985.1:WP_011383538.1 AMB_RS05720 argininosuccinate synthase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 467.8 0.0 1.8e-144 1.8e-144 1 390 [. 7 400 .. 7 403 .. 0.96 Alignments for each domain: == domain 1 score: 467.8 bits; conditional E-value: 1.8e-144 TIGR00032 1 kvvlaysGGlDtsvalklleek.gaeviavtvdvGqpeedldaieekalklGaeka.yvi.Dareefvk 66 kvvlaysGGlDts++l++l+++ +ev+++t+d+Gq+ e+l+ ++ ka +G ++ + i D reefv+ lcl|NCBI__GCF_000009985.1:WP_011383538.1 7 KVVLAYSGGLDTSIILRWLQDQyQCEVVTFTADLGQG-EELEPARAKARLMGIKEEnIFIdDLREEFVR 74 8*********************999***********9.99********999976541344379****** PP TIGR00032 67 dylfaaiqanavyegkYllstaLaRpliakklvevakkegaeavahGctgKGnDqvRFelsilllnpdl 135 d++f+ +ana+yeg+Yll+t++aRpli+k+++e+a+ ga+av+hG+tgKGnDqvRFe+ ++l+pd+ lcl|NCBI__GCF_000009985.1:WP_011383538.1 75 DFVFPMFRANALYEGVYLLGTSIARPLISKRQIEIANMVGADAVSHGATGKGNDQVRFEMGYYALKPDI 143 ********************************************************************* PP TIGR00032 136 kviaPvreleli.ReeeieyaaekGievpvek..ekaysiDenllgrsiEageLEdpsteppediyelv 201 kviaP+r ++l+ R++++++a+++ i+++ +k e +ys+D nll++s+E++ LEdp++ep ed+y+ lcl|NCBI__GCF_000009985.1:WP_011383538.1 144 KVIAPWRLWDLTsRTKLLDFAEKHQIPIAKDKrgEAPYSTDANLLHISYEGKALEDPWVEPFEDMYTRS 212 ***********99***************99875579********************************9 PP TIGR00032 202 kdpiektedepevveieFekGvPvalngeslepvelilkaneiagkhGvGriDivEdRiiglKsReiYE 270 + p++++ d+p ++eieFekG va++g++l+p l+ k+ne++g++G+Gr+D vE+R +g+KsR++YE lcl|NCBI__GCF_000009985.1:WP_011383538.1 213 VAPENAP-DKPTYIEIEFEKGDAVAIDGVRLSPAALLTKLNELGGANGIGRLDLVENRFVGMKSRGVYE 280 9787777.************************************************************* PP TIGR00032 271 apalllLikAhkdLetlvltkdvlefkeiveekyseliYkGlwfdplaealdalikktqervtGtvrvk 339 +p+ +Li Ah+++e+l+l++ + ++k+ +y+eliY+G+wf p++ a++++i+k+ e+v+Gtvr+k lcl|NCBI__GCF_000009985.1:WP_011383538.1 281 TPGGSVLIVAHRAMESLTLDRGESHLKDELMPRYAELIYNGFWFAPERIAIQTMIDKVSENVSGTVRLK 349 ********************************************************************* PP TIGR00032 340 lfkGnaivigrkseyslYdeelvsfekdkefdqkdaiGfikirglqikvyr 390 lfkG a v+grks++slY + v+fe+d+ +dq+da+Gfik+++l+++ + lcl|NCBI__GCF_000009985.1:WP_011383538.1 350 LFKGVASVVGRKSPKSLYRMDYVTFEEDSVYDQRDAQGFIKLNALRMRLAK 400 *********************************************998765 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (394 nodes) Target sequences: 1 (404 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.02u 0.01s 00:00:00.03 Elapsed: 00:00:00.01 # Mc/sec: 8.29 // [ok]
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory