Alignments for a candidate for hisN in Magnetospirillum magneticum AMB-1

GapMind for Amino acid biosynthesis

Alignments for a candidate for hisN in Magnetospirillum magneticum AMB-1

Align Histidinol-phosphatase [alternative form] (EC 3.1.3.15) (characterized)
to candidate WP_011386518.1 AMB_RS21100 histidinol-phosphatase

Query= reanno::azobra:AZOBR_RS03845
         (260 letters)



>NCBI__GCF_000009985.1:WP_011386518.1
          Length = 258

 Score =  318 bits (816), Expect = 5e-92
 Identities = 158/254 (62%), Positives = 189/254 (74%), Gaps = 1/254 (0%)

Query: 5   CPTPLVTLAERLADASGPVIRQYFRTPVAVDDKADASPVTIADREAERTIRAIIEAERPD 64
           CP+  + LAE+LADA+ PV+R+YFRTPVAVDDKAD+SPVTIADRE E  +RAI+ AE P 
Sbjct: 4   CPSEFIALAEKLADAARPVVRKYFRTPVAVDDKADSSPVTIADREVEAAMRAILAAEAPS 63

Query: 65  DGIYGEEFGTKNLDAEWVWVIDPIDGTKSFITGRPIFGTLIALLHRGRPVLGVIDQPIVR 124
            GI GEE G +N DAE+VWV+DPIDGT +FITG+P FGTLI+L   G P+LG+IDQ    
Sbjct: 64  HGILGEEHGRENCDAEYVWVLDPIDGTAAFITGKPSFGTLISLARNGTPILGLIDQAFTN 123

Query: 125 DRWLGVEGRPTLFNGQPARVRECAGGLAAATLGTTSPDLFPGADQDAFRRVAGAAKVSVY 184
           +RWLG  GRPT  NGQPARVR C   LA A   TT+P+LF  A + A+ R+A   K   Y
Sbjct: 124 ERWLGALGRPTTLNGQPARVRACP-DLAHAYAFTTAPELFCEATRPAWDRIAAGCKRPRY 182

Query: 185 GGDCYSYGLLAAGYYDLVVESGLKLYDFAALVPVVTGAGGLMTDWDGRPLDATSSGRVVA 244
           G DCY+Y LL++G+ DLVVE+GLK YDFAALVPVV GAGG+MTDW G+PL   S G+V A
Sbjct: 183 GCDCYAYALLSSGFCDLVVEAGLKPYDFAALVPVVEGAGGIMTDWSGKPLTIHSDGKVCA 242

Query: 245 AGDARTHRETLAAL 258
           AGD R H E L  L
Sbjct: 243 AGDRRLHAEALGVL 256


Lambda     K      H
   0.320    0.139    0.424 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 305
Number of extensions: 15
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 260
Length of database: 258
Length adjustment: 24
Effective length of query: 236
Effective length of database: 234
Effective search space:    55224
Effective search space used:    55224
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 47 (22.7 bits)

Align candidate WP_011386518.1 AMB_RS21100 (histidinol-phosphatase)
to HMM TIGR02067 (hisN: histidinol-phosphatase (EC 3.1.3.15))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR02067.hmm
# target sequence database:        /tmp/gapView.27740.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR02067  [M=252]
Accession:   TIGR02067
Description: his_9_HisN: histidinol-phosphatase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
    4.4e-93  297.4   0.0      5e-93  297.2   0.0    1.0  1  lcl|NCBI__GCF_000009985.1:WP_011386518.1  AMB_RS21100 histidinol-phosphata


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000009985.1:WP_011386518.1  AMB_RS21100 histidinol-phosphatase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  297.2   0.0     5e-93     5e-93       3     251 ..      10     256 ..       8     257 .. 0.97

  Alignments for each domain:
  == domain 1  score: 297.2 bits;  conditional E-value: 5e-93
                                 TIGR02067   3 alalelaeaageailkyfrasdlkvdkksdktpVteADraaEeaireliaakfPddgilGEEfgeeeed 71 
                                               ala++la+aa+ ++ kyfr+  ++vd k+d++pVt+ADr++E+a+r+++aa+ P++gilGEE+g e+ d
  lcl|NCBI__GCF_000009985.1:WP_011386518.1  10 ALAEKLADAARPVVRKYFRT-PVAVDDKADSSPVTIADREVEAAMRAILAAEAPSHGILGEEHGRENCD 77 
                                               799****************9.77888889999************************************* PP

                                 TIGR02067  72 aeyvWvlDPiDGTksFirGvPvwgtLiaLlekgkpvlGvisqPalgerfvaakgegallnggerelrvs 140
                                               aeyvWvlDPiDGT +Fi+G P +gtLi+L+++g+p+lG+i+q +++er+++a g+ ++lng  +++rv+
  lcl|NCBI__GCF_000009985.1:WP_011386518.1  78 AEYVWVLDPIDGTAAFITGKPSFGTLISLARNGTPILGLIDQAFTNERWLGALGRPTTLNG--QPARVR 144
                                               ************************************************************8..8***** PP

                                 TIGR02067 141 evaklsdAvlvttspkaledeeereafeklrrkarltryggdcyayalvAsGrvdlvveaelspyDiaa 209
                                               ++ +l++A+ +tt p +l+ e++r a++++++ +++ ryg+dcyayal+ sG+ dlvvea+l+pyD+aa
  lcl|NCBI__GCF_000009985.1:WP_011386518.1 145 ACPDLAHAYAFTTAP-ELFCEATRPAWDRIAAGCKRPRYGCDCYAYALLSSGFCDLVVEAGLKPYDFAA 212
                                               ***************.79999************************************************ PP

                                 TIGR02067 210 lipiieeAggvitdwkGkeae..eggeavaaanaalhdevlell 251
                                               l+p++e+Agg++tdw+Gk+++  ++g++ aa++++lh+e+l +l
  lcl|NCBI__GCF_000009985.1:WP_011386518.1 213 LVPVVEGAGGIMTDWSGKPLTihSDGKVCAAGDRRLHAEALGVL 256
                                               *******************999899***************9987 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (252 nodes)
Target sequences:                          1  (258 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.00
# Mc/sec: 6.98
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis