Alignments for a candidate for ilvC in Magnetospirillum magneticum AMB-1

GapMind for Amino acid biosynthesis

Alignments for a candidate for ilvC in Magnetospirillum magneticum AMB-1

Align acetohydroxyacid isomeroreductase (EC 1.1.1.86) (characterized)
to candidate WP_011385869.1 AMB_RS17750 ketol-acid reductoisomerase

Query= metacyc::MONOMER-18814
         (338 letters)



>NCBI__GCF_000009985.1:WP_011385869.1
          Length = 339

 Score =  460 bits (1183), Expect = e-134
 Identities = 227/339 (66%), Positives = 273/339 (80%), Gaps = 1/339 (0%)

Query: 1   MKVFYDKDADLSLIKGKNVTIIGYGSQGHAHALNLKDSGV-NVTVGLRKSGASWNKAANA 59
           M+V+YD+DAD++LIKGK V ++GYGSQGHAHALNL+DSGV +V V LR   A+  KA   
Sbjct: 1   MRVYYDRDADVNLIKGKKVVVVGYGSQGHAHALNLRDSGVKDVAVALRAGSATAKKAEGE 60

Query: 60  GLQVKEVAEAVKGADVVMILLPDEQIADVYKNEVHDNIKEGAALAFAHGFNVHYGAVIPR 119
           GL+V   A+A K  DVVMIL PDE  AD+Y N++  N+K+GA+L FAHG N+H+  +  R
Sbjct: 61  GLKVMTPADAAKWGDVVMILTPDELQADLYYNDLAANMKQGASLVFAHGLNIHFKLIEAR 120

Query: 120 ADLDVIMIAPKAPGHTVRATYTQGGGVPHLIAVHQNKSGAARDIALSYATANGGGRAGII 179
           ADLDV M+APK PGHTVR  Y +GGGVP L+AV QN SG A +IALSYA+A GGGR+GII
Sbjct: 121 ADLDVFMVAPKGPGHTVRGEYVKGGGVPCLVAVAQNASGNALEIALSYASAIGGGRSGII 180

Query: 180 ETNFREETETDLFGEQAVLCGGTVELIKAGFETLVEAGYAPEMAYFECLHELKLIVDLIY 239
           ET+FREE ETDLFGEQ VLCGG  +LI+ GFETLVEAGYAPEMAYFECLHE+KLIVDLIY
Sbjct: 181 ETSFREECETDLFGEQVVLCGGLTKLIQYGFETLVEAGYAPEMAYFECLHEVKLIVDLIY 240

Query: 240 EGGIANMNYSISNNAEYGEYVTGPRVVTEETKKAMKQCLTDIQTGEYAKSFLLENKAGAP 299
           EGGIANM YSISN AEYG+YVTGPR++T+ETK  MK+ L DIQ G + + ++LE KAG P
Sbjct: 241 EGGIANMRYSISNTAEYGDYVTGPRIITDETKAEMKRVLEDIQAGRFVRDWMLECKAGQP 300

Query: 300 TLISRRRLTAEHQIEEVGAKLRAMMPWIAKNKMVDQSKN 338
           +  + RR+ AEH IE VG KLRAMMPWIAKNK+VD++KN
Sbjct: 301 SFKATRRIQAEHGIEVVGEKLRAMMPWIAKNKLVDKAKN 339


Lambda     K      H
   0.316    0.133    0.379 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 428
Number of extensions: 16
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 338
Length of database: 339
Length adjustment: 28
Effective length of query: 310
Effective length of database: 311
Effective search space:    96410
Effective search space used:    96410
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 49 (23.5 bits)

Align candidate WP_011385869.1 AMB_RS17750 (ketol-acid reductoisomerase)
to HMM TIGR00465 (ilvC: ketol-acid reductoisomerase (EC 1.1.1.86))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00465.hmm
# target sequence database:        /tmp/gapView.29863.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00465  [M=314]
Accession:   TIGR00465
Description: ilvC: ketol-acid reductoisomerase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   2.3e-135  436.5   0.5   2.7e-135  436.3   0.5    1.0  1  lcl|NCBI__GCF_000009985.1:WP_011385869.1  AMB_RS17750 ketol-acid reductois


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000009985.1:WP_011385869.1  AMB_RS17750 ketol-acid reductoisomerase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  436.3   0.5  2.7e-135  2.7e-135       1     312 [.      14     327 ..      14     329 .. 0.99

  Alignments for each domain:
  == domain 1  score: 436.3 bits;  conditional E-value: 2.7e-135
                                 TIGR00465   1 lkgkkvaiiGyGsqGeaqalnlrdsgl.nvivglrkeaaswkkAeedGfkvltveeaikkadlimiLlp 68 
                                               +kgkkv+++GyGsqG+a+alnlrdsg+ +v v+lr ++a+ kkAe +G+kv+t ++a+k +d++miL+p
  lcl|NCBI__GCF_000009985.1:WP_011385869.1  14 IKGKKVVVVGYGSQGHAHALNLRDSGVkDVAVALRAGSATAKKAEGEGLKVMTPADAAKWGDVVMILTP 82 
                                               689************************67**************************************** PP

                                 TIGR00465  69 DevqkevyeaeikpllkegkallfsHGfnivfkqivipkdvdvvlvAPKgpGalvReeykegrGvpsli 137
                                               De+q + y ++++ ++k+g++l+f+HG ni+fk i++++d+dv++vAPKgpG++vR ey +g Gvp l+
  lcl|NCBI__GCF_000009985.1:WP_011385869.1  83 DELQADLYYNDLAANMKQGASLVFAHGLNIHFKLIEARADLDVFMVAPKGPGHTVRGEYVKGGGVPCLV 151
                                               ********************************************************************* PP

                                 TIGR00465 138 AveqdvtgeakeiAlayAkaiGgaragvlettFkeEvesDLfGEqavLcGglealikaafdtLveaGyq 206
                                               Av q+++g+a eiAl+yA aiGg+r g++et+F+eE+e+DLfGEq+vLcGgl++li+ +f+tLveaGy+
  lcl|NCBI__GCF_000009985.1:WP_011385869.1 152 AVAQNASGNALEIALSYASAIGGGRSGIIETSFREECETDLFGEQVVLCGGLTKLIQYGFETLVEAGYA 220
                                               ********************************************************************* PP

                                 TIGR00465 207 pelAyfeivhelklivdllkekGlelmrdavsntAklgalelr.eilkeelkkemqkilkeiqnGefak 274
                                               pe+Ayfe++he+klivdl++e+G+++mr ++sntA++g++ ++ +i+++e+k+em+ +l++iq G+f +
  lcl|NCBI__GCF_000009985.1:WP_011385869.1 221 PEMAYFECLHEVKLIVDLIYEGGIANMRYSISNTAEYGDYVTGpRIITDETKAEMKRVLEDIQAGRFVR 289
                                               *******************************************9************************* PP

                                 TIGR00465 275 ewalekeagkpafeearkkekeqeiekvGkelralvka 312
                                               +w+le +ag+p+f+++r+ + e+ ie vG++lra++++
  lcl|NCBI__GCF_000009985.1:WP_011385869.1 290 DWMLECKAGQPSFKATRRIQAEHGIEVVGEKLRAMMPW 327
                                               ************************************97 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (314 nodes)
Target sequences:                          1  (339 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.00
# Mc/sec: 11.30
//
[ok]

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis