Align Probable 2-isopropylmalate synthase; EC 2.3.3.13; Alpha-IPM synthase; Alpha-isopropylmalate synthase (uncharacterized)
to candidate WP_011384529.1 AMB_RS10755 citramalate synthase
Query= curated2:Q8TYB1 (499 letters) >NCBI__GCF_000009985.1:WP_011384529.1 Length = 546 Score = 224 bits (571), Expect = 6e-63 Identities = 179/548 (32%), Positives = 263/548 (47%), Gaps = 52/548 (9%) Query: 1 MPDRVRIFDTTLRDGEQTPGVSLTVEEKVEIARKLDEFGVDTIEAGFPVASEGE---FEA 57 M +RV ++DTTLRDG QT GV + +KV IAR+LD G+D +E G+P A+ + F Sbjct: 1 MAERVYLYDTTLRDGAQTQGVDFSAADKVRIARELDRIGIDYVEGGWPGANPTDDAFFAD 60 Query: 58 VRAIAGEELDAEICGLARCV--KGDIDAAIDADVDC---VHVFIATS-DIHLRYKLEMSR 111 A L A G+ R D D ++A + V + S D + L + Sbjct: 61 PPAFKRSRLTA--FGMTRRAGRSADNDPGLNALLVTPARVATMVGKSWDFQVTVALGIEL 118 Query: 112 EEALERAIEGVEYASDHGVTVEFSAE---DATRTDRDYLLEVYKATVEAGADRVNVPDTV 168 +E L + V++A V F AE D + + Y L KA +AGA V + DT Sbjct: 119 DENLRMVADSVQHARTKVEEVMFDAEHFFDGYKANPAYALAAAKAAYDAGARWVVLCDTN 178 Query: 169 GVMTPPEMYRLTAEVVDAVDVPVS---VHCHNDFGMAVANSLAAVEAGAEQVHVTVNGIG 225 G P EM R+ EV+ +P S VHCHND AVANSLAAVEAG Q+ T+NG+G Sbjct: 179 GGTLPHEMRRIVGEVIKG-GIPGSHLGVHCHNDSDTAVANSLAAVEAGVRQIQGTLNGLG 237 Query: 226 ERAGNASLEQVVMALKALYDIELDVRTE---MLVELSRLVERLTGVVVPPNTPIVGENAF 282 ER GNA+L ++ AL + + E LV +SR ++ + P VG++AF Sbjct: 238 ERCGNANLISIIPALMLKMGFDTGIAPEDLKNLVSVSRALDEVLDRTPNRGQPYVGKSAF 297 Query: 283 AHESGIHSHGVIKKAETYEPIRPEDVGHRRRIVLGKHAGRHAIKKKLEEMGIEVTEEQLD 342 AH+ G+H V K + YE + P VG+ R IV+ AGR + +L E+GI++ + + Sbjct: 298 AHKGGLHVSAVAKDPKCYEHVDPASVGNMRHIVMSDQAGRSNLVARLTEIGIDLDSVKRE 357 Query: 343 EIVRRV------------KELGDKGKRVTED---------DLEAIARDVVGEVPESEAAV 381 +VR V +L D KR+ + E + R +GEVPE A Sbjct: 358 SLVRVVDQMQVTFDAREATDLVDLVKRLEHEGYAYDQAAASFELLVRRALGEVPEYFALE 417 Query: 382 KLEEIAVMTGNKF-----TPTASVRVYLDGEEHEAASTGVGSV---DAAIRALREAIEEL 433 + I N A+V+V + G E+ G G V D A+R + + Sbjct: 418 RYRVIDERRRNAHGEWITLSEATVKVEVGGTEYMEVGEGTGPVHAFDVALRKVLTRVYPA 477 Query: 434 GMDVELKEYRLEAI--TGGTDALAEVTVRLEDEDGNVTTARGAAEDIVMASVKAFVRGVN 491 +ELK+YR+ T GT A VT+ ED+ G+ T G +++ AS++A + Sbjct: 478 LTALELKDYRVRITESTVGTAAKTRVTIESEDDKGHRWTTVGVHTNVLEASLEALQDSIT 537 Query: 492 RLARRRRD 499 + R +D Sbjct: 538 FMLFRFKD 545 Lambda K H 0.315 0.133 0.364 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 632 Number of extensions: 34 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 499 Length of database: 546 Length adjustment: 35 Effective length of query: 464 Effective length of database: 511 Effective search space: 237104 Effective search space used: 237104 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.5 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory