GapMind for catabolism of small carbon sources

 

Protein 7024268 in Shewanella sp. ANA-3

Annotation: FitnessBrowser__ANA3:7024268

Length: 436 amino acids

Source: ANA3 in FitnessBrowser

Candidate for 19 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-isoleucine catabolism fadA hi 3-ketoacyl-CoA thiolase FadI; ACSs; Acetyl-CoA acyltransferase; Acyl-CoA ligase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta; EC 2.3.1.16 (characterized) 72% 99% 634.8 3-keto-acyl-CoA-thiolase 34% 229.2
L-isoleucine catabolism fadA hi fadI: acetyl-CoA C-acyltransferase FadI (EC 2.3.1.16) (TIGR02446) 100% 913.1 3-keto-acyl-CoA-thiolase 34% 229.2
4-hydroxybenzoate catabolism atoB med Acetyl-CoA acetyltransferase; EC 2.3.1.9; Acetoacetyl-CoA thiolase (uncharacterized) 35% 99% 231.9 3-keto-acyl-CoA-thiolase 34% 229.2
L-arginine catabolism atoB med Acetyl-CoA acetyltransferase; EC 2.3.1.9; Acetoacetyl-CoA thiolase (uncharacterized) 35% 99% 231.9 3-keto-acyl-CoA-thiolase 34% 229.2
L-citrulline catabolism atoB med Acetyl-CoA acetyltransferase; EC 2.3.1.9; Acetoacetyl-CoA thiolase (uncharacterized) 35% 99% 231.9 3-keto-acyl-CoA-thiolase 34% 229.2
2-deoxy-D-ribonate catabolism atoB med Acetyl-CoA acetyltransferase; EC 2.3.1.9; Acetoacetyl-CoA thiolase (uncharacterized) 35% 99% 231.9 3-keto-acyl-CoA-thiolase 34% 229.2
2-deoxy-D-ribose catabolism atoB med Acetyl-CoA acetyltransferase; EC 2.3.1.9; Acetoacetyl-CoA thiolase (uncharacterized) 35% 99% 231.9 3-keto-acyl-CoA-thiolase 34% 229.2
L-leucine catabolism atoB med Acetyl-CoA acetyltransferase; EC 2.3.1.9; Acetoacetyl-CoA thiolase (uncharacterized) 35% 99% 231.9 3-keto-acyl-CoA-thiolase 34% 229.2
L-lysine catabolism atoB med Acetyl-CoA acetyltransferase; EC 2.3.1.9; Acetoacetyl-CoA thiolase (uncharacterized) 35% 99% 231.9 3-keto-acyl-CoA-thiolase 34% 229.2
phenylacetate catabolism atoB med Acetyl-CoA acetyltransferase; EC 2.3.1.9; Acetoacetyl-CoA thiolase (uncharacterized) 35% 99% 231.9 3-keto-acyl-CoA-thiolase 34% 229.2
L-phenylalanine catabolism atoB med Acetyl-CoA acetyltransferase; EC 2.3.1.9; Acetoacetyl-CoA thiolase (uncharacterized) 35% 99% 231.9 3-keto-acyl-CoA-thiolase 34% 229.2
L-proline catabolism atoB med Acetyl-CoA acetyltransferase; EC 2.3.1.9; Acetoacetyl-CoA thiolase (uncharacterized) 35% 99% 231.9 3-keto-acyl-CoA-thiolase 34% 229.2
L-tyrosine catabolism atoB med Acetyl-CoA acetyltransferase; EC 2.3.1.9; Acetoacetyl-CoA thiolase (uncharacterized) 35% 99% 231.9 3-keto-acyl-CoA-thiolase 34% 229.2
4-hydroxybenzoate catabolism paaJ2 lo β-ketoadipyl-CoA thiolase (EC 2.3.1.174; EC 2.3.1.223) (characterized) 31% 98% 183.7 3-ketoacyl-CoA thiolase FadI; ACSs; Acetyl-CoA acyltransferase; Acyl-CoA ligase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta; EC 2.3.1.16 72% 634.8
4-hydroxybenzoate catabolism pcaF lo β-ketoadipyl-CoA thiolase (EC 2.3.1.174; EC 2.3.1.223) (characterized) 31% 98% 183.7 3-ketoacyl-CoA thiolase FadI; ACSs; Acetyl-CoA acyltransferase; Acyl-CoA ligase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta; EC 2.3.1.16 72% 634.8
phenylacetate catabolism paaJ1 lo β-ketoadipyl-CoA thiolase (EC 2.3.1.174; EC 2.3.1.223) (characterized) 31% 98% 183.7 3-ketoacyl-CoA thiolase FadI; ACSs; Acetyl-CoA acyltransferase; Acyl-CoA ligase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta; EC 2.3.1.16 72% 634.8
phenylacetate catabolism paaJ2 lo β-ketoadipyl-CoA thiolase (EC 2.3.1.174; EC 2.3.1.223) (characterized) 31% 98% 183.7 3-ketoacyl-CoA thiolase FadI; ACSs; Acetyl-CoA acyltransferase; Acyl-CoA ligase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta; EC 2.3.1.16 72% 634.8
L-phenylalanine catabolism paaJ1 lo β-ketoadipyl-CoA thiolase (EC 2.3.1.174; EC 2.3.1.223) (characterized) 31% 98% 183.7 3-ketoacyl-CoA thiolase FadI; ACSs; Acetyl-CoA acyltransferase; Acyl-CoA ligase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta; EC 2.3.1.16 72% 634.8
L-phenylalanine catabolism paaJ2 lo β-ketoadipyl-CoA thiolase (EC 2.3.1.174; EC 2.3.1.223) (characterized) 31% 98% 183.7 3-ketoacyl-CoA thiolase FadI; ACSs; Acetyl-CoA acyltransferase; Acyl-CoA ligase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta; EC 2.3.1.16 72% 634.8
L-tryptophan catabolism pcaF lo β-ketoadipyl-CoA thiolase (EC 2.3.1.174; EC 2.3.1.223) (characterized) 31% 98% 183.7 3-ketoacyl-CoA thiolase FadI; ACSs; Acetyl-CoA acyltransferase; Acyl-CoA ligase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta; EC 2.3.1.16 72% 634.8

Sequence Analysis Tools

View 7024268 at FitnessBrowser

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MSDRQQVTNAKGERIAIVAGLRTPFAKQATAFHGVSALDMGKMVVNELLARSELDPKLIE
QLVYGQVVQMPAAPNIAREIVLGTGMDVSTDAYSVTRACATSFQSAVNVAESIMTGNIEI
GIAGGADSSSVLPIGVSKKLAHALVDLNKARSFGQKLQIFRRLGIKDLLPVPPAVAEYST
GLSMGQTAEQMAKTYNISRADQDALAHRSHTLASETWASGHLRDEVMVAHVPPYKQFIER
DNNIRENSDLSSYAKLRPAFDKKHGSVTAANSTPLTDGASAIILMSEGRAKALGYQPIGY
IKSYAFTAIDVWQDMLMGPSYATPLALKRAGMELEDLTLIEMHEAFAAQTLANMQMFASK
KFAEEKLGRNRAIGEIDMSKFNVLGGSLAYGHPFAATGTRLITQVCRELKRRGGGTGLAT
ACAAGGLGAAMIVEVE

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory