Align The SnatA carrier. Transports glycine, L-alanine, L-serine, L-threonine and a variety of neutral L-amino acids (characterized)
to candidate 7024611 Shewana3_1789 hypothetical protein (RefSeq)
Query= TCDB::Q8J305 (216 letters) >FitnessBrowser__ANA3:7024611 Length = 210 Score = 148 bits (374), Expect = 6e-41 Identities = 82/213 (38%), Positives = 131/213 (61%), Gaps = 8/213 (3%) Query: 2 LEVVEFLKYLILLYGGLFAITNPVGAVPVFLSVTHDLSWRERREIASKTAISVVATLVVF 61 +E+ ++K+ + GL AI NP+G +PVF+S+T + ER +VV L+V Sbjct: 1 MELTLYVKFFL----GLVAIINPIGLLPVFVSLTSHQTEAERNHTGKVANFAVVVILLVT 56 Query: 62 ALLGQWIFKFFGSSTDAFAIAGGILLFRMALDMLSGKLSSVKISNEETEEFSEEVVTLEE 121 + GQ I F S AF IAGG L+ +A+ ML GKL VK + EE E S +E Sbjct: 57 IVAGQHILNMFSISLSAFRIAGGTLIAIIAMSMLQGKLGEVKRNQEEDRESS----AMES 112 Query: 122 VAIIPLAIPLISGPGAITTVMLYMAKSTTNLQRLAVILTIILIGITVWFVLCSANRIKAR 181 VA++PLA+PL++GPGAI++V+++ A+ + +A+ +T+I+ G+ + + A+ I Sbjct: 113 VAVVPLALPLMAGPGAISSVIVFAAEHNSFSNFVAMFITVIIFGLVSFALFRMASIIYKI 172 Query: 182 LGRVGIKVMTRMMGLILTSMAVQMIINGIKGAF 214 LG+ GI V+TR+MGL++ S+ +++I GIKG F Sbjct: 173 LGKTGINVITRLMGLLMLSIGIEVIAAGIKGLF 205 Lambda K H 0.327 0.141 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 115 Number of extensions: 5 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 216 Length of database: 210 Length adjustment: 22 Effective length of query: 194 Effective length of database: 188 Effective search space: 36472 Effective search space used: 36472 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 45 (21.9 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory