Align CbtF, component of Cellobiose and cellooligosaccharide porter (characterized)
to candidate 7025948 Shewana3_3096 spermidine/putrescine ABC transporter ATPase subunit (RefSeq)
Query= TCDB::Q97VF4 (324 letters) >FitnessBrowser__ANA3:7025948 Length = 378 Score = 124 bits (312), Expect = 3e-33 Identities = 72/211 (34%), Positives = 127/211 (60%), Gaps = 20/211 (9%) Query: 3 LMELKGVSVIFEDKVGLFKKRKFYALKDVSLSMNQGDLLIVLGESGAGKTTLGRVIVGLQ 62 L++++ VS +F+D A+ DVSL++N+G++ +LG SG+GK+TL R++ G + Sbjct: 20 LLKIERVSKLFDD---------VRAVDDVSLTINKGEIFALLGGSGSGKSTLLRMLAGFE 70 Query: 63 KPTSGEVVYDGYNIWKNKRKIFKKYRKDVQLIPQDPYSTLPFNKTVEEILVAPILRWEKI 122 +PTSG + DG +I Y + + ++ Q Y+ P + I L+ +K+ Sbjct: 71 RPTSGRIFLDGEDITD-----LPPYERPINMMFQS-YALFPHMTVAQNIAFG--LKQDKL 122 Query: 123 NKDELRKRLINLLELVKLTPAEEFLGKYPHQLSGGQKQRLSIARSLSVNPRIIVADEPVT 182 K E+ +R+ +L+LV + E++ + PHQLSGGQ+QR+++ARSL+ P++++ DEP+ Sbjct: 123 PKAEIEQRVQEMLKLVHM---EQYGKRKPHQLSGGQRQRVALARSLAKRPKLLLLDEPMG 179 Query: 183 MVDASLRIGILNTLAEIKNRLNLTMVFITHD 213 +D LR + + EI R+ +T V +THD Sbjct: 180 ALDKKLRTQMQLEVVEILERVGVTCVMVTHD 210 Lambda K H 0.321 0.141 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 253 Number of extensions: 12 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 324 Length of database: 378 Length adjustment: 29 Effective length of query: 295 Effective length of database: 349 Effective search space: 102955 Effective search space used: 102955 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 49 (23.5 bits)
This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory