Alignments for a candidate for H281DRAFT_01113 in Shewanella sp. ANA-3

GapMind for catabolism of small carbon sources

Alignments for a candidate for H281DRAFT_01113 in Shewanella sp. ANA-3

Align deoxynucleoside transporter, ATPase component (characterized)
to candidate 7024900 Shewana3_2074 ABC transporter related (RefSeq)

Query= reanno::Burk376:H281DRAFT_01113
         (515 letters)



>FitnessBrowser__ANA3:7024900
          Length = 499

 Score =  306 bits (785), Expect = 9e-88
 Identities = 179/504 (35%), Positives = 290/504 (57%), Gaps = 21/504 (4%)

Query: 16  LEVVGVHKRFTGVHALRGVSLSFQRGQIYHLLGENGCGKSTLIKIISGAQPPDEGQLVIE 75
           LE+  + K + GV AL  VSL    G+++ LLGENG GKSTL+K+++GAQ  D G ++  
Sbjct: 5   LELKQISKHYPGVKALEDVSLRLFAGEVHALLGENGAGKSTLVKVMTGAQSKDMGDILFL 64

Query: 76  GVPHARLSALEALAAGIETVYQDLSLLPNMSVAENVALTSELATHEGRLARTFDRRVLAA 135
           G P    + ++A  AGI TVYQ+++L+PN++VA+N+ L      +E R       + + A
Sbjct: 65  GEPQHFNTPMDAQKAGISTVYQEVNLVPNLTVAQNLFLG-----YEPRRLGLIHFKKMYA 119

Query: 136 TAARALEAVGLPGNSEFQSTLIEQLPLATRQLVAIARAIASEAKFVIMDEPTTSLTQKEV 195
            A   L    L  +    S  +    +A +QL+AIAR +A  AK +++DEPT SL  KEV
Sbjct: 120 DARAVLTQFKLDIDV---SAPLSDYSIAVQQLIAIARGVAMSAKVLVLDEPTASLDAKEV 176

Query: 196 DNLIAVLANLRAQGVTVLFVSHKLDECYAIGGEVIVLRDGQKMAQGPIAEFTKAQISELM 255
             L  +L  L+A+GV ++F++H LD+ Y I   + VLR+GQ + +   AE  + ++ E M
Sbjct: 177 QVLFGILNQLKAKGVAIVFITHFLDQVYQISDRITVLRNGQFIGEYLTAELPQPKLIEAM 236

Query: 256 TGRHLSN------ERYRESAHAQDIVLDVRGFTRAGQFSDVSFKLHGGEILGVTGLLDSG 309
            GR L        E+ R    A+ ++L +   +  G    ++  +  G+ +G+ GLL SG
Sbjct: 237 LGRSLQEQLVDKQEKERTVTRAEAVLLSLEDVSVKGSIQSMNLTVPKGQAVGLAGLLGSG 296

Query: 310 RNELARALAGVAPAQSGDVLLDGQQIALRTPSDAKRHRIGYVPEDRLNEGLFLDKPIRDN 369
           R+E+  A+ G+    SG + L GQ++ L  P DA    I   PEDR  +G+     IR+N
Sbjct: 297 RSEVCNAVFGLDLVDSGSIHLAGQKLNLSQPVDAISAGIALCPEDRKIDGIIGPLSIREN 356

Query: 370 VITAMISSLRDRFG---QIDRTRAQALAEQTVKELQIATPGVDKPVQSLSGGNQQRVLIG 426
           +I A    L+ R G    +  TR Q +A+  + +LQIATP  DKP++ LSGGNQQ+V++ 
Sbjct: 357 IILA----LQARIGWWRYLSNTRQQEIAQFFIDKLQIATPDADKPIEQLSGGNQQKVILA 412

Query: 427 RWLAIDPRVLILHGPTVGVDVGSKDIIYRIMQRLSQRGIGIILISDDLPELLQNCDRILM 486
           RWLAI+P +L+L  PT G+D+G+   I ++++ L   G+ +++ S +L EL+   +++++
Sbjct: 413 RWLAIEPILLVLDEPTRGIDIGAHAEIVKLIRTLCDEGMSLLVASSELDELVAFSNKVVV 472

Query: 487 MKKGHVSAEYRADELSEADLYHAL 510
           ++  +   E    EL+   +  A+
Sbjct: 473 LRDRYAVRELSGAELTSQHVMQAI 496



 Score = 89.0 bits (219), Expect = 4e-22
 Identities = 61/233 (26%), Positives = 105/233 (45%), Gaps = 21/233 (9%)

Query: 289 DVSFKLHGGEILGVTGLLDSGRNELARALAGVAPAQSGDVLLDGQQIALRTPSDAKR--- 345
           DVS +L  GE+  + G   +G++ L + + G      GD+L  G+     TP DA++   
Sbjct: 22  DVSLRLFAGEVHALLGENGAGKSTLVKVMTGAQSKDMGDILFLGEPQHFNTPMDAQKAGI 81

Query: 346 ----HRIGYVPEDRLNEGLFLDKPIRDNVITAMISSLRDRFGQIDRTRAQALAEQTVKEL 401
                 +  VP   + + LFL    R             R G I   +  A A   + + 
Sbjct: 82  STVYQEVNLVPNLTVAQNLFLGYEPR-------------RLGLIHFKKMYADARAVLTQF 128

Query: 402 QIATPGVDKPVQSLSGGNQQRVLIGRWLAIDPRVLILHGPTVGVDVGSKDIIYRIMQRLS 461
           ++    V  P+   S   QQ + I R +A+  +VL+L  PT  +D     +++ I+ +L 
Sbjct: 129 KLDID-VSAPLSDYSIAVQQLIAIARGVAMSAKVLVLDEPTASLDAKEVQVLFGILNQLK 187

Query: 462 QRGIGIILISDDLPELLQNCDRILMMKKGHVSAEYRADELSEADLYHALLSEA 514
            +G+ I+ I+  L ++ Q  DRI +++ G    EY   EL +  L  A+L  +
Sbjct: 188 AKGVAIVFITHFLDQVYQISDRITVLRNGQFIGEYLTAELPQPKLIEAMLGRS 240


Lambda     K      H
   0.319    0.135    0.376 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 591
Number of extensions: 36
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 515
Length of database: 499
Length adjustment: 34
Effective length of query: 481
Effective length of database: 465
Effective search space:   223665
Effective search space used:   223665
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources