GapMind for catabolism of small carbon sources

 

Alignments for a candidate for tpi in Shewanella sp. ANA-3

Align triose-phosphate isomerase (EC 5.3.1.1) (characterized)
to candidate 7026209 Shewana3_3351 phosphoglycerate kinase (RefSeq)

Query= BRENDA::P36204
         (654 letters)



>FitnessBrowser__ANA3:7026209
          Length = 391

 Score =  328 bits (840), Expect = 4e-94
 Identities = 180/399 (45%), Positives = 261/399 (65%), Gaps = 12/399 (3%)

Query: 1   MEKMTIRDVDLKGKRVIMRVDFNVPVKDGVVQDDTRIRAALPTIKYALEQGAKVILLSHL 60
           M  + + D+DL+GKRV++R D NVPV +GVV  D R+RA+LPTI+ AL +GA V+++SHL
Sbjct: 1   MAIINMSDLDLQGKRVLIREDLNVPVSNGVVTSDARLRASLPTIELALAKGAAVMVMSHL 60

Query: 61  GRP-KGEPSPEFSLAPVAKRLSELLGKEVKFVPAVVGDEVKKAVEELKEGEVLLLENTRF 119
           GRP +GE + EFS+ PV   L++ L   V+     + D V+ AV     GEV++ EN RF
Sbjct: 61  GRPTEGEYNSEFSMQPVVDYLAKALSCPVRLATDYL-DGVEVAV-----GEVVVFENVRF 114

Query: 120 HPGETKNDPELAKFWASLADIHVNDAFGTAHRAHASNVGIAQFIP-SVAGFLMEKEIKFL 178
           + GE KND  L+K  A+L D++V DAFGTAHRA AS  G+    P + AG L+ +E++ L
Sbjct: 115 NKGEKKNDEALSKKMAALCDVYVMDAFGTAHRAEASTNGVGLHAPIACAGPLLAQELEAL 174

Query: 179 SKVTYNPEKPYVVVLGGAKVSDKIGVITNLMEKADRILIGGAMMFTFLKALGKEVGSSRV 238
            K   NP +P V ++GG+KVS K+ V+ +L    D++++GG +  TF+ A G  VG S  
Sbjct: 175 GKALDNPARPLVAIVGGSKVSTKLTVLESLSGIVDQLVVGGGIANTFIAAAGHNVGKSLY 234

Query: 239 EEDKIDLAKELLEKAKEKGVEIVLPVDAVIAQKIEPGVEKKVVRIDDGIPEGWMGLDIGP 298
           E D ID AK L+  A+ +G +I +P D V+A +  P     +  +++ + +  M  DIGP
Sbjct: 235 EADLIDEAKRLVANAQSRGGDIPVPTDVVVAGEFSPTAAATLKAVNE-VGDSDMIFDIGP 293

Query: 299 ETIELFKQKLSDAKTVVWNGPMGVFEIDDFAEGTKQVALAIAALTEKGAITVVGGGDSAA 358
           ++ E   + +  A T+VWNGP+GVFE D F EGTK++A AIA   +  A ++ GGGD+ A
Sbjct: 294 DSAEALAKIIESAGTIVWNGPVGVFEFDQFGEGTKRIAQAIA---DSKAFSIAGGGDTLA 350

Query: 359 AVNKFGLEDKFSHVSTGGGASLEFLEGKELPGIASIADK 397
           AV+K+ + DK S++STGGGA LEFLEGKELP +A +  +
Sbjct: 351 AVDKYDIADKVSYISTGGGAFLEFLEGKELPAVAMLKQR 389


Lambda     K      H
   0.317    0.137    0.386 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 594
Number of extensions: 41
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 654
Length of database: 391
Length adjustment: 34
Effective length of query: 620
Effective length of database: 357
Effective search space:   221340
Effective search space used:   221340
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory