Alignments for a candidate for dgoD in Shewanella sp. ANA-3

GapMind for catabolism of small carbon sources

Alignments for a candidate for dgoD in Shewanella sp. ANA-3

Align L-arabinonate dehydratase; ArDHT; D-fuconate dehydratase; Galactonate dehydratase; L-arabonate dehydratase; EC 4.2.1.25; EC 4.2.1.67; EC 4.2.1.6 (characterized)
to candidate 7023120 Shewana3_0358 dihydroxy-acid dehydratase (RefSeq)

Query= SwissProt::B5ZZ34
         (579 letters)



>FitnessBrowser__ANA3:7023120
          Length = 619

 Score =  241 bits (614), Expect = 8e-68
 Identities = 181/548 (33%), Positives = 265/548 (48%), Gaps = 68/548 (12%)

Query: 43  GRPVIGILNTWSDMTPCNGHLRELAEKVKAGVWEAGGFPLEVPVFSASEN-TFRPTAMMY 101
           G+P+I I N+++   P + HL+++   V   + EAGG   E    +  +        M+Y
Sbjct: 33  GKPIIAIANSFTQFVPGHVHLKDMGSLVADAIEEAGGIAKEFNTIAVDDGIAMGHGGMLY 92

Query: 102 ----RNLAALAVEEAIRGQPMDGCVLLVGCDKTTPSLLMGAASCDLPSIVVTGGPMLNGY 157
               R L A +VE  +     D  V +  CDK TP +LM A   ++P + V+GGPM  G 
Sbjct: 93  SLPSRELIADSVEYMVNAHCADALVCISNCDKITPGMLMAALRLNIPVVFVSGGPMEAG- 151

Query: 158 FRGERVGSGTHLWKFSEMVKAGEMTQAEFLEAEASMSRSS----GTCNTMGTASTMASMA 213
            + +       L     MV A + + ++  E  A + RS+    G+C+ M TA++M  + 
Sbjct: 152 -KTKLSDKLIKLDLVDAMVAAADSSVSD--EDSAKIERSACPTCGSCSGMFTANSMNCLT 208

Query: 214 EALGMALSGNAAIPGVDSRRKVMAQLTGRRIVQMVK-----DDLK--PSEIMTKQAFENA 266
           EALG++L GN ++    + R+ +    GRR++ + K     DD    P  I + +AFENA
Sbjct: 209 EALGLSLPGNGSMLATHADRRELFLEAGRRVMALTKRYYEQDDASALPRNIASFKAFENA 268

Query: 267 IRTNAAIGGSTNAVIHLLAIAGRVGIDLSLDDWDRCGRDVPTIVNLMPS-GKYLMEEFFY 325
           +  + A+GGS+N V+HLLA A    +  ++DD DR  R VP +  + PS  KY ME+   
Sbjct: 269 MALDIAMGGSSNTVLHLLAAAQEADVAFTMDDIDRMSRQVPHLCKVAPSTAKYHMEDVHR 328

Query: 326 AGGLPVVLKRLGEAGLLHKDALTVSG----------------ETVWDEVKDV-------- 361
           AGG+  +L  L  AGLLH D   V+                 +T  D+VK          
Sbjct: 329 AGGVMGILGELDRAGLLHTDVPHVAADAGGNLKSVLAKYDVMQTQDDKVKQFFMAGPAGI 388

Query: 362 ---------VNW-------NEDVILPAEKALTSSGGIVVLRGNLAPKGAVLKPSAASPHL 405
                      W        E  I   E A +  GG+ VL GNLA  G ++K +      
Sbjct: 389 PTTKAFSQDCRWPSLDDDRREGCIRSREFAFSQEGGLAVLSGNLADNGCIVKTAGVDESN 448

Query: 406 LVHKGRAVVFEDIDDYKAKINDDNLDIDENCIMVMKNCGPKGYPGMAEVGNMGLPPKVLK 465
           L   G A V+E  DD  A I     ++    ++V++  GPKG PGM E   M  P   LK
Sbjct: 449 LTFIGSARVYESQDDAVAGILGG--EVVAGDVVVIRYEGPKGGPGMQE---MLYPTSYLK 503

Query: 466 KGILD--MVRISDARMSGTAYGTVVLHTSPEAAVGGPLAVVKNGDMIELDVPNRRLHLDI 523
              L      I+D R SG   G  + H SPEAA GG +A+++NGD IE+D+P R + L +
Sbjct: 504 SRGLGKACALITDGRFSGGTSGLSIGHVSPEAAAGGTIALIENGDRIEIDIPKRSIKLAV 563

Query: 524 SDEELARR 531
           SD ELA R
Sbjct: 564 SDAELAAR 571


Lambda     K      H
   0.318    0.135    0.408 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 910
Number of extensions: 43
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 579
Length of database: 619
Length adjustment: 37
Effective length of query: 542
Effective length of database: 582
Effective search space:   315444
Effective search space used:   315444
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 17 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources